Diversity of Cationic Antimicrobial Peptides in Black Cumin (Nigella sativa L.) Seeds

Black cumin (Nigella sativa L.) is known to possess a wide variety of antimicrobial peptides belonging to different structural families. Three novel antimicrobial peptides have been isolated from black cumin seeds. Two of them were attributed as members of the non-specific lipid transfer proteins family, and one as a defensin. We have made an attempt of using the proteomic approach for novel antimicrobial peptides search in N. sativa seeds as well. The use of a well-established approach that includes extraction and fractionation stages remains relevant even in the case of novel peptides search because of the lacking N. sativa genome data. Novel peptides demonstrate a spectrum of antimicrobial activity against plant pathogenic organisms that may cause economically important crop diseases. These results obtained allow considering these molecules as candidates to be applied in “next-generation” biopesticides development for agricultural use.

[1]  Tina Austerlitz,et al.  The thionin family of antimicrobial peptides , 2021, PloS one.

[2]  M. Berezovski,et al.  Characterization and differentiation of quinoa seed proteomes by label-free mass spectrometry-based shotgun proteomics. , 2021, Food chemistry.

[3]  Xingyong Yang,et al.  Plant antimicrobial peptides: structures, functions, and applications , 2021, Botanical studies.

[4]  R. Van Ree,et al.  The diagnosis and management of allergic reactions in patients sensitized to non‐specific lipid transfer proteins , 2021, Allergy.

[5]  E. Rogozhin,et al.  Nigellothionins from Black Cumin (Nigella sativa L.) Seeds Demonstrate Strong Antifungal and Cytotoxic Activity , 2021, Antibiotics.

[6]  Chenchen Wang,et al.  A nonspecific lipid transfer protein, StLTP10, mediates resistance to Phytophthora infestans in potato , 2020, Molecular plant pathology.

[7]  E. Rogozhin,et al.  Isolation of antimicrobial peptides from different plant sources: Does a general extraction method exist? , 2020, Plant methods.

[8]  E. Rogozhin,et al.  Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease , 2020, Plants.

[9]  D. Vlachakis,et al.  Proteome analysis of leaf, stem and callus in Viscum album and identification of lectins and viscotoxins with bioactive properties , 2020, Plant Cell, Tissue and Organ Culture (PCTOC).

[10]  D. Craik,et al.  Cyclotides: disulfide-rich peptide toxins in plants. , 2019, Toxicon : official journal of the International Society on Toxinology.

[11]  G. Labesse,et al.  Comprehensive classification of the plant non-specific lipid transfer protein superfamily towards its sequence–structure–function analysis , 2019, PeerJ.

[12]  Marilyn A. Anderson,et al.  The evolution, function and mechanisms of action for plant defensins. , 2019, Seminars in cell & developmental biology.

[13]  T. Ovchinnikova,et al.  Peptides of the Innate Immune System of Plants. Part II. Biosynthesis, Biological Functions, and Possible Practical Applications , 2019, Russian Journal of Bioorganic Chemistry.

[14]  T. Ovchinnikova,et al.  Peptides of the Innate Immune System of Plants. Part I. Structure, Biological Activity, and Mechanisms of Action , 2018, Russian Journal of Bioorganic Chemistry.

[15]  S. Sekaran,et al.  Antimicrobial peptides from different plant sources: Isolation, characterisation, and purification. , 2018, Phytochemistry.

[16]  Chengsheng Meng,et al.  Systematic Analysis of Cotton Non-specific Lipid Transfer Protein Family Revealed a Special Group That Is Involved in Fiber Elongation , 2018, Front. Plant Sci..

[17]  A. A. Souza,et al.  Lipid transfer protein isolated from noni seeds displays antibacterial activity in vitro and improves survival in lethal sepsis induced by CLP in mice. , 2018, Biochimie.

[18]  S. Zavriev,et al.  Novel Thionins from Black Seed (Nigella sativa L.) Demonstrate Antimicrobial Activity , 2017, International Journal of Peptide Research and Therapeutics.

[19]  I. M. Vasconcelos,et al.  First isolation and antinociceptive activity of a lipid transfer protein from noni (Morinda citrifolia) seeds. , 2016, International journal of biological macromolecules.

[20]  Eugene I. Rumynskiy,et al.  A novel lipid transfer protein from the pea Pisum sativum: isolation, recombinant expression, solution structure, antifungal activity, lipid binding, and allergenic properties , 2016, BMC Plant Biology.

[21]  T. Ovchinnikova,et al.  Lipid Transfer Proteins As Components of the Plant Innate Immune System: Structure, Functions, and Applications , 2016, Acta naturae.

[22]  James P. Tam,et al.  Antimicrobial Peptides from Plants , 2015, Pharmaceuticals.

[23]  E. Grishin,et al.  A novel antifungal peptide from leaves of the weed Stellaria media L. , 2015, Biochimie.

[24]  E. Grishin,et al.  Defense peptides from barnyard grass (Echinochloa crusgalli L.) seeds , 2012, Peptides.

[25]  R. Van Ree,et al.  Lentil (Lens culinaris) Lipid Transfer Protein Len c 3: A Novel Legume Allergen , 2011, International Archives of Allergy and Immunology.

[26]  N. Cedergreen,et al.  Biomedicine in the environment: Cyclotides constitute potent natural toxins in plants and soil bacteria , 2011, Environmental toxicology and chemistry.

[27]  E. Grishin,et al.  Isolation, molecular cloning and antimicrobial activity of novel defensins from common chickweed (Stellaria media L.) seeds. , 2011, Biochimie.

[28]  M. Mann,et al.  Andromeda: a peptide search engine integrated into the MaxQuant environment. , 2011, Journal of proteome research.

[29]  E. Grishin,et al.  Novel antifungal defensins from Nigella sativa L. seeds. , 2011, Plant physiology and biochemistry : PPB.

[30]  E. Grishin,et al.  A novel antifungal hevein‐type peptide from Triticum kiharae seeds with a unique 10‐cysteine motif , 2009, The FEBS journal.

[31]  E. Grishin,et al.  Isolation of the lipid-transporting protein Ns-LTP1 from seeds of the garden fennel flower (Nigella sativa) , 2009, Russian Journal of Bioorganic Chemistry.

[32]  B. Hwang,et al.  Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco , 2009, Plant Cell Reports.

[33]  A. Slusarenko,et al.  Effects of garlic (Allium sativum) juice containing allicin on Phytophthora infestans and downy mildew of cucumber caused by Pseudoperonospora cubensis , 2008, European Journal of Plant Pathology.

[34]  M. Gautier,et al.  Genome-wide analysis of the rice and arabidopsis non-specific lipid transfer protein (nsLtp) gene families and identification of wheat nsLtp genes by EST data mining , 2008, BMC Genomics.

[35]  J. Rose,et al.  The biochemistry and biology of extracellular plant lipid‐transfer proteins (LTPs) , 2008, Protein science : a publication of the Protein Society.

[36]  E. Grishin,et al.  Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes. , 2007, Biochimie.

[37]  Xingyong Yang,et al.  Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds , 2006, Peptides.

[38]  M. Gautier,et al.  Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds. , 2005, Biochimica et biophysica acta.

[39]  K. Silverstein,et al.  Genome Organization of More Than 300 Defensin-Like Genes in Arabidopsis1[w] , 2005, Plant Physiology.

[40]  M. Regente,et al.  The cytotoxic properties of a plant lipid transfer protein involve membrane permeabilization of target cells , 2005, Letters in applied microbiology.

[41]  Eva Kondorosi,et al.  A Novel Family in Medicago truncatula Consisting of More Than 300 Nodule-Specific Genes Coding for Small, Secreted Polypeptides with Conserved Cysteine Motifs1,212 , 2003, Plant Physiology.

[42]  R. Dixon,et al.  A putative lipid transfer protein involved in systemic resistance signalling in Arabidopsis , 2002, Nature.

[43]  R. Thoma,et al.  Purification and characterization of three antifungal proteins from cheeseweed (Malva parviflora). , 2001, Biochemical and biophysical research communications.

[44]  Sang Yeol Lee,et al.  Characterization and cDNA cloning of two glycine- and histidine-rich antimicrobial peptides from the roots of shepherd's purse, Capsella bursa-pastoris , 2000, Plant Molecular Biology.

[45]  M. Mena,et al.  Differential expression of pathogen-responsive genes encoding two types of glycine-rich proteins in barley , 1997, Plant Molecular Biology.

[46]  W. Broekaert,et al.  Mutational Analysis of a Plant Defensin from Radish (Raphanus sativus L.) Reveals Two Adjacent Sites Important for Antifungal Activity* , 1997, The Journal of Biological Chemistry.

[47]  A. Molina,et al.  Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens , 1993, FEBS letters.

[48]  Franky R. G. Terras,et al.  In Vitro Antifungal Activity of a Radish (Raphanus sativus L.) Seed Protein Homologous to Nonspecific Lipid Transfer Proteins. , 1992, Plant physiology.

[49]  Franky R. G. Terras,et al.  Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. , 1992, The Journal of biological chemistry.

[50]  W. Gray,et al.  A toxic thionin from Pyrularia pubera: purification, properties, and amino acid sequence. , 1985, Archives of biochemistry and biophysics.

[51]  Marilyn A. Anderson,et al.  Plant defensins: Common fold, multiple functions , 2013 .

[52]  Mahabir P. Gupta,et al.  Cyclotide proteins and precursors from the genus Gloeospermum: filling a blank spot in the cyclotide map of Violaceae. , 2010, Phytochemistry.

[53]  L. Bohlin,et al.  Small, novel proteins from the mistletoe Phoradendron tomentosum exhibit highly selective cytotoxicity to human breast cancer cells , 2003, Cellular and Molecular Life Sciences CMLS.