Coupling a DNA-ligand ensemble with Ag cluster formation for the label-free and ratiometric detection of intracellular biothiols.

We have demonstrated the synthesis of a smart nanoprobe by coupling a DNA-ligand ensemble with Ag cluster formation that allows the label-free and ratiometric detection of intracellular biothiols with high sensitivity and selectivity as well as discrimination of normal cells and cancer cells.

[1]  A. Ajayaghosh,et al.  Self-assembled near-infrared dye nanoparticles as a selective protein sensor by activation of a dormant fluorophore. , 2014, Journal of the American Chemical Society.

[2]  Yu-Fen Huang,et al.  Analysis of adenosine triphosphate and glutathione through gold nanoparticles assisted laser desorption/ionization mass spectrometry. , 2007, Analytical chemistry.

[3]  Jin‐Ming Lin,et al.  Determination of cysteine, homocysteine, cystine, and homocystine in biological fluids by HPLC using fluorosurfactant-capped gold nanoparticles as postcolumn colorimetric reagents. , 2014, Journal of separation science.

[4]  Hideaki Mizuno,et al.  Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters , 2010, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[5]  D. Townsend,et al.  S-glutathionylation: from molecular mechanisms to health outcomes. , 2011, Antioxidants & redox signaling.

[6]  S. Nair,et al.  Luminescent quantum clusters of gold in bulk by albumin-induced core etching of nanoparticles: metal ion sensing, metal-enhanced luminescence, and biolabeling. , 2010, Chemistry.

[7]  R. Dickson,et al.  Tailoring silver nanodots for intracellular staining , 2011, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[8]  Yaodong Zhang,et al.  A label-free DNA-templated silver nanocluster probe for fluorescence on-off detection of endonuclease activity and inhibition. , 2014, Biosensors & bioelectronics.

[9]  Sudha Seshadri,et al.  Plasma Homocysteine as a Risk Factor for Dementia and Alzheimer's Disease , 2002 .

[10]  J. Ho,et al.  Facile preparation of high-quantum-yield gold nanoclusters: application to probing mercuric ions and biothiols. , 2014, ACS applied materials & interfaces.

[11]  Run Zhang,et al.  A lanthanide complex-based ratiometric luminescence probe for time-gated luminescence detection of intracellular thiols. , 2013, Analytical chemistry.

[12]  X. Qu,et al.  One-step DNA-programmed growth of CpG conjugated silver nanoclusters: a potential platform for simultaneous enhanced immune response and cell imaging. , 2013, Chemical communications.

[13]  Xiaodong Xia,et al.  Hairpin DNA probe with 5'-TCC/CCC-3' overhangs for the creation of silver nanoclusters and miRNA assay. , 2014, Biosensors & bioelectronics.

[14]  Chang Su Lim,et al.  Ratiometric detection of mitochondrial thiols with a two-photon fluorescent probe. , 2011, Journal of the American Chemical Society.

[15]  J. Zen,et al.  Electrocatalytic oxidation and sensitive detection of cysteine on a lead ruthenate pyrochlore modified electrode , 2001 .

[16]  N. Murthy,et al.  Fluorescent coumarin thiols measure biological redox couples. , 2012, Organic letters.

[17]  Jason J. Han,et al.  A DNA--silver nanocluster probe that fluoresces upon hybridization. , 2010, Nano letters.

[18]  Weihong Tan,et al.  Ag nanocluster-based label-free catalytic and molecular beacons for amplified biosensing. , 2015, Chemical communications.

[19]  I. Willner,et al.  Aptasensors based on supramolecular structures of nucleic acid-stabilized Ag nanoclusters. , 2015, Chemical communications.

[20]  Y V Tcherkas,et al.  Simultaneous determination of several amino acids, including homocysteine, cysteine and glutamic acid, in human plasma by isocratic reversed-phase high-performance liquid chromatography with fluorimetric detection. , 2001, Journal of chromatography. A.

[21]  Liyan Zheng,et al.  Sensitive detection of mercury and copper ions by fluorescent DNA/Ag nanoclusters in guanine-rich DNA hybridization. , 2015, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[22]  S. Shahrokhian,et al.  Lead phthalocyanine as a selective carrier for preparation of a cysteine-selective electrode. , 2001, Analytical chemistry.

[23]  D. Leong,et al.  Glutathione-protected silver nanoclusters as cysteine-selective fluorometric and colorimetric probe. , 2013, Analytical chemistry.

[24]  D. Townsend,et al.  Sulfur containing amino acids and human disease. , 2004, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[25]  Jin‐Ming Lin,et al.  High-performance liquid chromatography assay of cysteine and homocysteine using fluorosurfactant-functionalized gold nanoparticles as postcolumn resonance light scattering reagents. , 2013, Journal of Chromatography A.

[26]  Z. Li,et al.  A label-free method for detecting biothiols based on poly(thymine)-templated copper nanoparticles. , 2015, Biosensors & bioelectronics.

[27]  A. Puga,et al.  Regulation of gene expression by reactive oxygen. , 1999, Annual review of pharmacology and toxicology.

[28]  Lin Yuan,et al.  Construction of a FRET-based ratiometric fluorescent thiol probe. , 2011, Chemical communications.

[29]  Z. A. Wood,et al.  Structure, mechanism and regulation of peroxiredoxins. , 2003, Trends in biochemical sciences.

[30]  I. Willner,et al.  Multiplexed analysis of genes using nucleic acid-stabilized silver-nanocluster quantum dots. , 2014, ACS nano.

[31]  Yajun Wang,et al.  Carbon‐Dot‐Based Nanosensors for the Detection of Intracellular Redox State , 2015, Advanced materials.

[32]  W. Tan,et al.  A novel AgNP/DNA/TPdye conjugate-based two-photon nanoprobe for GSH imaging in cell apoptosis of cancer tissue. , 2015, Chemical communications.

[33]  Xin Meng,et al.  Role of oxidative stress and intracellular glutathione in the sensitivity to apoptosis induced by proteasome inhibitor in thyroid cancer cells , 2009, BMC Cancer.

[34]  S. Oja,et al.  Mechanisms of L-Cysteine Neurotoxicity , 2000, Neurochemical Research.

[35]  J. Chao,et al.  Coordination-mediated programmable assembly of unmodified oligonucleotides on plasmonic silver nanoparticles. , 2015, ACS applied materials & interfaces.

[36]  Q. Dong,et al.  Assay of glutathione in individual mouse peritoneal macrophages by capillary zone electrophoresis with electrochemical detection. , 2000, Analytical biochemistry.

[37]  I. Warner,et al.  Visual detection of cysteine and homocysteine. , 2004, Journal of the American Chemical Society.

[38]  Weiwei Guo,et al.  Highly sequence-dependent formation of fluorescent silver nanoclusters in hybridized DNA duplexes for single nucleotide mutation identification. , 2010, Journal of the American Chemical Society.

[39]  X. Qu,et al.  Highly sensitive and selective detection of thiol-containing biomolecules using DNA-templated silver deposition. , 2011, Biosensors & bioelectronics.

[40]  R. Dickson,et al.  Generation of luminescent noble metal nanodots in cell matrices , 2012, Photochemical & Photobiological Sciences.

[41]  H. Ju,et al.  DNA-regulated silver nanoclusters for label-free ratiometric fluorescence detection of DNA. , 2014, Chemical communications.

[42]  S. Threlfell,et al.  Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine. , 2001, The Analyst.

[43]  Xiaogang Qu,et al.  DNA-templated silver nanoparticles as a platform for highly sensitive and selective fluorescence turn-on detection of dopamine. , 2011, Small.

[44]  Li Shang,et al.  Intracellular thermometry by using fluorescent gold nanoclusters. , 2013, Angewandte Chemie.

[45]  Xiaogang Qu,et al.  Modulating DNA-templated silver nanoclusters for fluorescence turn-on detection of thiol compounds. , 2011, Chemical communications.

[46]  Hsin-Chih Yeh,et al.  A fluorescence light-up Ag nanocluster probe that discriminates single-nucleotide variants by emission color. , 2012, Journal of the American Chemical Society.

[47]  Chen-Ho Tung,et al.  BODIPY-based ratiometric fluorescent sensor for highly selective detection of glutathione over cysteine and homocysteine. , 2012, Journal of the American Chemical Society.

[48]  D. Jacobsen.  Homocysteine and vitamins in cardiovascular disease. , 1998, Clinical chemistry.

[49]  M. Bruns,et al.  Ultrasmall fluorescent silver nanoclusters: Protein adsorption and its effects on cellular responses , 2012, Nano Research.

[50]  I. M. Robertson,et al.  Silver cluster formation, dynamics, and chemistry in metal-organic frameworks. , 2009, Nano letters.

[51]  E. Wang,et al.  Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids. , 2011, Biosensors & bioelectronics.

[52]  Shaomin Ji,et al.  Colorimetric and ratiometric fluorescent chemosensor based on diketopyrrolopyrrole for selective detection of thiols: an experimental and theoretical study. , 2011, The Journal of organic chemistry.

[53]  Xiaoling Zhang,et al.  A colorimetric and ratiometric fluorescent probe for thiols and its bioimaging applications. , 2010, Chemical communications.

[54]  R. Dickson,et al.  Shuttle-based fluorogenic silver-cluster biolabels. , 2009, Angewandte Chemie.

[55]  Zhihong Liu,et al.  Construction of an upconversion nanoprobe with few-atom silver nanoclusters as the energy acceptor. , 2015, Angewandte Chemie.