Dye-Free Porcine Model of Experimental Branch Retinal Vein Occlusion: A Suitable Approach for Retinal Proteomics

Branch retinal vein occlusion induces complex biological processes in the retina that are generated by a multitude of interacting proteins. These proteins and their posttranslational modifications can effectively be studied using modern proteomic techniques. However, no method for studying large-scale protein changes following branch retinal vein occlusion has been available until now. Obtainment of retinal tissue exposed to branch retinal vein occlusion is only available through experimental animal models. Traditional models of experimental branch retinal vein occlusion require the use of Rose Bengal dye combined with argon laser photocoagulation. The use of Rose Bengal dye is problematic in proteomic studies as the dye can induce multiple protein modifications when irradiated. This paper presents a novel technique for proteomic analysis of porcine retinal tissue with branch retinal vein occlusion combining a dye-free experimental model with label-free liquid chromatography mass spectrometry based proteomics.

[1]  I. Menon,et al.  A study on the sequence of phototoxic effects of rose bengal using retinal pigment epithelial cells in vitro. , 1989, Experimental eye research.

[2]  B. Honoré,et al.  Analytical platforms in vitreoretinal proteomics. , 2014, Bioanalysis.

[3]  Francisco Tirado,et al.  GeneCodis: interpreting gene lists through enrichment analysis and integration of diverse biological information , 2009, Nucleic Acids Res..

[4]  M. Davies,et al.  Photo-oxidation-induced inactivation of the selenium-containing protective enzymes thioredoxin reductase and glutathione peroxidase. , 2012, Free radical biology & medicine.

[5]  R D Sperduto,et al.  Arteriovenous crossing patterns in branch retinal vein occlusion. The Eye Disease Case-Control Study Group. , 1993, Ophthalmology.

[6]  S. Olsson,et al.  Ultrasound enhanced thrombolysis in experimental retinal vein occlusion in the rabbit , 1998, The British journal of ophthalmology.

[7]  D. Charteris,et al.  Animal models of retinal detachment and reattachment: identifying cellular events that may affect visual recovery , 2002, Eye.

[8]  M. Larsen,et al.  Comorbidity in patients with branch retinal vein occlusion: case-control study , 2012, BMJ : British Medical Journal.

[9]  M. Harith,et al.  Photosensitized Effects of Rose Bengal on Structure and Function of Lens Protein “Alpha‐Crystallin” , 2009, Photochemistry and photobiology.

[10]  C. Pournaras,et al.  Decreased nitric oxide production accounts for secondary arteriolar constriction after retinal branch vein occlusion. , 1997, Investigative ophthalmology & visual science.

[11]  Dao-Yi Yu,et al.  Intravitreal tenecteplase (metalyse) for acute management of retinal vein occlusions. , 2013, Investigative ophthalmology & visual science.

[12]  M. Gillies,et al.  Proteome changes induced by laser in diabetic retinopathy , 2015, Clinical & experimental ophthalmology.

[13]  Cathy Frey,et al.  Investigative Ophthalmology and Visual Science , 2010 .

[14]  T. Mimura,et al.  Association of inflammatory factors with macular edema in branch retinal vein occlusion. , 2013, JAMA ophthalmology.

[15]  Alberto D. Pascual-Montano,et al.  GeneCodis3: a non-redundant and modular enrichment analysis tool for functional genomics , 2012, Nucleic Acids Res..

[16]  C. Nordgaard,et al.  Human retinal pigment epithelium proteome changes in early diabetes , 2008, Diabetologia.

[17]  A. D. de Almeida,et al.  Pig proteomics: a review of a species in the crossroad between biomedical and food sciences. , 2012, Journal of proteomics.

[18]  M. Kim,et al.  In Vitro Study for Staining and Toxicity of Rose Bengal on Cultured Bovine Corneal Endothelial Cells , 1996, Cornea.

[19]  B. Honoré,et al.  Proteomic analysis of human vitreous associated with idiopathic epiretinal membrane , 2013, Acta ophthalmologica.

[20]  S. Tseng,et al.  Interaction Between Rose Bengal and Different Protein Components , 1995, Cornea.

[21]  W. Bubb,et al.  Singlet Oxygen–mediated Protein Oxidation: Evidence for the Formation of Reactive Side Chain Peroxides on Tyrosine Residues ¶ , 2002, Photochemistry and photobiology.

[22]  A. Tonnel,et al.  Endothelial cells , 1991 .

[23]  Dao-Yi Yu,et al.  Effect of triamcinolone acetonide on vascular endothelial growth factor and occludin levels in branch retinal vein occlusion. , 2009, American journal of ophthalmology.

[24]  M. Davies.  Reactive species formed on proteins exposed to singlet oxygen , 2004, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[25]  P. Mitchell,et al.  Natural history of branch retinal vein occlusion: an evidence-based systematic review. , 2010, Ophthalmology.

[26]  B. Honoré,et al.  Proteomic analysis as a means to approach limbal stem cell biology in a search for stem cell markers , 2014, Proteomics. Clinical applications.

[27]  J. Carazo,et al.  GENECODIS: a web-based tool for finding significant concurrent annotations in gene lists , 2007, Genome Biology.

[28]  Matus Rehak,et al.  Branch Retinal Vein Occlusion: Pathogenesis, Visual Prognosis, and Treatment Modalities , 2008, Current eye research.

[29]  Henrik Vorum,et al.  Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry , 2009, Biological Procedures Online.

[30]  M. la Cour,et al.  Electrophysiological consequences of experimental branch retinal vein occlusion in pigs and the effect of dorzolamide. , 2011, Investigative ophthalmology & visual science.