论文信息 - Crystallization properties and structure of Panulirus interruptus haemocyanin.

Crystallization properties and structure of Panulirus interruptus haemocyanin.

Electron-microscopic studies revealed that two types of subunits of Panulirus interruptus haemocyanin crystallize in different ways. Homohexamers of subunit a give close-packed two-dimensional crystals whereas homohexamers of subunit c form open two-dimensional arrays. We applied computer-image analysis to these arrays and studied the differences in crystallization properties by combining the electron-microscopic data with amino acid sequence information and the X-ray diffraction model of subunit a.

[1] P. Jekel,et al. Panulirus interruptus hemocyanin. The amino acid sequence of subunit b and anomalous behaviour of subunits a and b on polyacrylamide gel electrophoresis in the presence of SDS. , 1988, European journal of biochemistry.

[2] R. Henderson,et al. Images of purple membrane at 2.8 A resolution obtained by cryo-electron microscopy. , 1988, Journal of molecular biology.

[3] J. Beintema,et al. Panulirus interruptus hemocyanin. The elucidation of the complete amino acid sequence of subunit a. , 1987, European journal of biochemistry.

[4] W. Hol,et al. Structure determination of Panulirus interruptus haemocyanin at 3.2 A resolution. Successful phase extension by sixfold density averaging. , 1986, Journal of molecular biology.

[5] Jones Ta,et al. Diffraction methods for biological macromolecules. Interactive computer graphics: FRODO. , 1985, Methods in enzymology.

[6] W. Hol,et al. 3.2 Å structure of the copper-containing, oxygen-carrying protein Panulirus interruptus haemocyanin , 1984, Nature.

[7] Marin van Heel,et al. IMAGIC - A FAST, FLEXIBLE AND FRIENDLY IMAGE-ANALYSIS SOFTWARE SYSTEM , 1981 .

[8] W. Keegstra,et al. A Simple Way of Making a 2-D Array , 1980 .