论文信息 - Individual identification in Romanian sheep breeds using microsatellite markers.

Individual identification in Romanian sheep breeds using microsatellite markers.

Genetic diversity, inbreeding level and parentage analysis can be important tools in the conservation of wild and endangered species which exhibit higher heterozygosity levels. Individual heterozygosity is more closely related to the degree of inbreeding. The aim of this study was to analyze the genetic characteristics of sheep breeds in Romania through the use of microsatellite molecular markers. Consequently, we have selected ten microsatellite loci that were amplified by multiplex PCR reaction in order to estimate the differences between individuals due to their high allelic variation. We have obtained a correct amplification for all microsatellites.

[1] M. Bruford,et al. Genetic diversity and subdivision of 57 European and Middle-Eastern sheep breeds. , 2007, Animal genetics.

[2] M. Mukesh,et al. Characterizing Nali and Chokla sheep differentiation with microsatellite markers , 2006 .

[3] F. Goyache,et al. Genetic relationships and admixture among sheep breeds from Northern Spain assessed using microsatellites. , 2004, Journal of animal science.

[4] A. Aguirre,et al. Tracking diversity and differentiation in six sheep breeds from the North Iberian Peninsula through DNA variation , 2004 .

[5] J. Arranz,et al. Differentiation among Spanish sheep breeds using microsatellites , 2001, Genetics Selection Evolution.

[6] P. Mathur,et al. Genetic distinctness of African, Asian and South American local chickens. , 2000, Animal genetics.

[7] J. Arranz,et al. Genetic relationships among Spanish sheep using microsatellites. , 1998, Animal genetics.

[8] D. Pollock,et al. Launching microsatellites: a review of mutation processes and methods of phylogenetic interference. , 1997, The Journal of heredity.

[9] S. Kalinowski,et al. Genetic variation of major histocompatibility complex and microsatellite loci: a comparison in bighorn sheep. , 1997, Genetics.