Three ways to make a vesicle

Cargo molecules have to be included in carrier vesicles of different forms and sizes to be transported between organelles. During this process, a limited set of proteins, including the coat proteins COPI, COPII and clathrin, carries out a programmed set of sequential interactions that lead to the budding of vesicles. A general model to explain the formation of coated vesicles is starting to emerge but the picture is more complex than we had imagined.

[1]  P. Evans,et al.  A Structural Explanation for the Binding of Multiple Ligands by the α-Adaptin Appendage Domain , 1999, Cell.

[2]  E. Ungewickell,et al.  Identification of the universal cofactor (auxilin 2) in clathrin coat dissociation. , 2000, European journal of cell biology.

[3]  J. Hinshaw,et al.  Dynamin Undergoes a GTP-Dependent Conformational Change Causing Vesiculation , 1998, Cell.

[4]  K. Porter,et al.  YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L , 1964, The Journal of cell biology.

[5]  R. Fletterick,et al.  Clathrin self-assembly is mediated by a tandemly repeated superhelix , 1999, Nature.

[6]  H. Riezman,et al.  The absence of Emp24p, a component of ER‐derived COPII‐coated vesicles, causes a defect in transport of selected proteins to the Golgi. , 1995, The EMBO journal.

[7]  G. Payne,et al.  Adaptor complex-independent clathrin function in yeast. , 1999, Molecular biology of the cell.

[8]  R. G. Anderson.  The caveolae membrane system. , 1998, Annual review of biochemistry.

[9]  C. Barlowe.  Traffic COPs of the Early Secretory Pathway , 2000, Traffic.

[10]  R. Schekman,et al.  SEC12 encodes a guanine-nucleotide-exchange factor essential for transport vesicle budding from the ER , 1993, Nature.

[11]  T. Graham,et al.  An arf1Delta synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport in Saccharomyces cerevisiae. , 1998, Genetics.

[12]  N Grigorieff,et al.  Clathrin coats at 21 Å resolution: a cellular assembly designed to recycle multiple membrane receptors , 1998, The EMBO journal.

[13]  J. Bonifacino,et al.  Interaction of tyrosine-based sorting signals with clathrin-associated proteins. , 1995, Science.

[14]  T. Kirchhausen.  Adaptors for clathrin-mediated traffic. , 1999, Annual review of cell and developmental biology.

[15]  S. Emr,et al.  Yeast epsins contain an essential N‐terminal ENTH domain, bind clathrin and are required for endocytosis , 1999, The EMBO journal.

[16]  M. Caron,et al.  The Interaction of β-Arrestin with the AP-2 Adaptor Is Required for the Clustering of β2-Adrenergic Receptor into Clathrin-coated Pits* , 2000, The Journal of Biological Chemistry.

[17]  J. Shim,et al.  Molecular genetic analysis of apm-2 and aps-2, genes encoding the medium and small chains of the AP-2 clathrin-associated protein complex in the nematode Caenorhabditis elegans. , 2000, Molecules and cells.

[18]  K. von Figura,et al.  μ1A‐adaptin‐deficient mice: lethality, loss of AP‐1 binding and rerouting of mannose 6‐phosphate receptors , 2000, The EMBO journal.

[19]  H. Riezman,et al.  Clathrin functions in the absence of heterotetrameric adaptors and AP180‐related proteins in yeast , 1999, The EMBO journal.

[20]  M. Overduin,et al.  Structure and Asn-Pro-Phe binding pocket of the Eps15 homology domain. , 1998, Science.

[21]  B. Glick,et al.  Dynamics of transitional endoplasmic reticulum sites in vertebrate cells. , 2000, Molecular biology of the cell.

[22]  J. Zimmerberg.  Are The Curves in all the Right Places? , 2000, Traffic.

[23]  C. Kaiser.  Thinking about p24 proteins and how transport vesicles select their cargo. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[24]  P. Evans,et al.  A structural explanation for the recognition of tyrosine-based endocytotic signals. , 1998, Science.

[25]  H. Jäckle,et al.  Role of Drosophila α-Adaptin in Presynaptic Vesicle Recycling , 1997, Cell.

[26]  K. von Figura,et al.  Early Embryonic Death of Mice Deficient in γ-Adaptin* , 1999, The Journal of Biological Chemistry.

[27]  M. Robinson,et al.  Cytosolic and Membrane-associated Proteins Involved in the Recruitment of AP-1 Adaptors onto the Trans-Golgi Network* , 1996, The Journal of Biological Chemistry.

[28]  J. Bonifacino,et al.  Association of the AP-3 adaptor complex with clathrin. , 1998, Science.

[29]  J. Rothman,et al.  Protein Sorting by Transport Vesicles , 1996, Science.

[30]  J. Rothman,et al.  An enzyme that removes clathrin coats: purification of an uncoating ATPase , 1984, The Journal of cell biology.

[31]  P. De Camilli,et al.  Synaptojanin 1: localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15 , 1997, FEBS letters.

[32]  E. Eisenberg,et al.  Role of auxilin in uncoating clathrin-coated vesicles , 1995, Nature.

[33]  P. De Camilli,et al.  Epsin 1 Undergoes Nucleocytosolic Shuttling and Its Eps15 Interactor Nh2-Terminal Homology (Enth) Domain, Structurally Similar to Armadillo and Heat Repeats, Interacts with the Transcription Factor Promyelocytic Leukemia Zn2+ Finger Protein (Plzf) , 2000, The Journal of cell biology.

[34]  S. Harrison,et al.  Peptide-in-groove interactions link target proteins to the beta-propeller of clathrin. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[35]  J. Goldberg.  Structural and Functional Analysis of the ARF1–ARFGAP Complex Reveals a Role for Coatomer in GTP Hydrolysis , 1999, Cell.

[36]  E. Eisenberg,et al.  Role of Cyclin G-associated Kinase in Uncoating Clathrin-coated Vesicles from Non-neuronal Cells* , 2000, The Journal of Biological Chemistry.

[37]  T. Blundell,et al.  Crystal structure of the pleckstrin homology domain from dynamin , 1994, Nature Structural Biology.

[38]  The EH1 domain of Eps15 is structurally classified as a member of the S100 subclass of EF-hand-containing proteins. , 1999, Biochemistry.

[39]  M. Caron,et al.  Role of β-Arrestin in Mediating Agonist-Promoted G Protein-Coupled Receptor Internalization , 1996, Science.

[40]  J. Lippincott-Schwartz,et al.  Kinetic Analysis of Secretory Protein Traffic and Characterization of Golgi to Plasma Membrane Transport Intermediates in Living Cells , 1998, The Journal of cell biology.

[41]  A. Dautry‐Varsat,et al.  The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP-2 , 1995, The Journal of cell biology.

[42]  L. Brodin,et al.  Endophilin/SH3p4 Is Required for the Transition from Early to Late Stages in Clathrin-Mediated Synaptic Vesicle Endocytosis , 1999, Neuron.

[43]  E. W. Jones,et al.  Clathrin requirement for normal growth of yeast. , 1987, Science.

[44]  C. L. Ball,et al.  Targeting and mistargeting of plasma membrane adaptors in vitro , 1993, The Journal of cell biology.

[45]  J. Shorter,et al.  Membrane traffic: Do cones mark sites of fission? , 2000, Current Biology.

[46]  R. Schekman,et al.  A Primer on Vesicle Budding , 1999, Cell.

[47]  T. Kirchhausen.  Coated pits and coated vesicles — sorting it all out , 1993 .

[48]  J. Rothman,et al.  The binding of AP-1 clathrin adaptor particles to Golgi membranes requires ADP-ribosylation factor, a small GTP-binding protein , 1993, Cell.

[49]  J. Benovic,et al.  Arrestin/Clathrin Interaction , 1997, The Journal of Biological Chemistry.

[50]  Francesca Santini,et al.  Spatial control of coated-pit dynamics in living cells , 1999, Nature Cell Biology.

[51]  Jie Zhang,et al.  The β2-adrenergic receptor/βarrestin complex recruits the clathrin adaptor AP-2 during endocytosis , 1999 .

[52]  R. Schekman,et al.  Coat Proteins and Vesicle Budding , 1996, Science.

[53]  R. Schekman,et al.  COPII: A membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum , 1994, Cell.

[54]  David Y. Thomas,et al.  gp25L/emp24/p24 Protein Family Members of the cis-Golgi Network Bind Both COP I and II Coatomer , 1998, The Journal of cell biology.

[55]  P. De Camilli,et al.  AP-2 recruitment to synaptotagmin stimulated by tyrosine-based endocytic motifs. , 1999, Science.

[56]  G. Payne,et al.  A role for clathrin in the sorting of vacuolar proteins in the Golgi complex of yeast. , 1992, The EMBO journal.

[57]  P R Evans,et al.  The structure and function of the β2‐adaptin appendage domain , 2000, The EMBO journal.

[58]  H. McMahon,et al.  Crystal structure of the amphiphysin‐2 SH3 domain and its role in the prevention of dynamin ring formation , 1998, The EMBO journal.

[59]  D. Gallwitz,et al.  Evidence for Overlapping and Distinct Functions in Protein Transport of Coat Protein Sec24p Family Members* , 2000, The Journal of Biological Chemistry.

[60]  S. Harrison,et al.  Functional organization of clathrin in coats: combining electron cryomicroscopy and X-ray crystallography. , 1999, Molecular cell.

[61]  P. Finan,et al.  Synaptojanin Is the Major Constitutively Active Phosphatidylinositol-3,4,5-trisphosphate 5-Phosphatase in Rodent Brain* , 1997, The Journal of Biological Chemistry.

[62]  S. Hunt,et al.  Amphiphysin heterodimers: potential role in clathrin-mediated endocytosis. , 1997, Molecular biology of the cell.

[63]  F. Wieland,et al.  Nonclathrin coat protein gamma, a subunit of coatomer, binds to the cytoplasmic dilysine motif of membrane proteins of the early secretory pathway. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[64]  T. Kanaseki,et al.  A Morphological Study of the Coated Vesicle Isolated from the Nerve Endings of the Guinea Pig Brain, with Special Reference to the Mechanism of Membrane Movements , 1969 .

[65]  S. Schmid,et al.  Impairment of dynamin's GAP domain stimulates receptor-mediated endocytosis , 1999, Nature.

[66]  G. Sprague,,et al.  Clathrin facilitates the internalization of seven transmembrane segment receptors for mating pheromones in yeast , 1993, The Journal of cell biology.

[67]  J. Bonifacino,et al.  Sequence requirements for the recognition of tyrosine‐based endocytic signals by clathrin AP‐2 complexes. , 1996, The EMBO journal.

[68]  T. O'Halloran,et al.  Spatially regulated recruitment of clathrin to the plasma membrane during capping and cell translocation. , 2000, Molecular biology of the cell.

[69]  J. Benovic,et al.  Arrestin/Clathrin Interaction , 1997, The Journal of Biological Chemistry.

[70]  S. Harrison,et al.  Configuration of clathrin trimers: evidence from electron microscopy. , 1986, Journal of ultrastructure and molecular structure research.

[71]  R. Crowther,et al.  Assembly and packing of clathrin into coats , 1981, The Journal of cell biology.

[72]  T. Kirchhausen.  Cell biology: Boa constrictor or rattlesnake? , 1999, Nature.

[73]  R. Schekman,et al.  Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs. , 1998, Science.

[74]  T. Kirchhausen,et al.  Eps15 Is a Component of Clathrin-coated Pits and Vesicles and Is Located at the Rim of Coated Pits* , 1996, The Journal of Biological Chemistry.

[75]  B. Pearse,et al.  Clathrin: anatomy of a coat protein. , 1999, Trends in cell biology.

[76]  J. Goldberg.  Decoding of Sorting Signals by Coatomer through a GTPase Switch in the COPI Coat Complex , 2000, Cell.

[77]  F. Maxfield,et al.  Membrane transport in the endocytic pathway. , 1995, Current opinion in cell biology.

[78]  R Pepperkok,et al.  COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites. , 2000, Journal of cell science.

[79]  L. Traub,et al.  ADP-ribosylation factor 1 transiently activates high-affinity adaptor protein complex AP-1 binding sites on Golgi membranes. , 1998, Molecular biology of the cell.

[80]  R. Cerione,et al.  Endocytosis: Is dynamin a ‘blue collar’ or ‘white collar’ worker? , 1999, Current Biology.

[81]  J. Benovic,et al.  β-Arrestin acts as a clathrin adaptor in endocytosis of the β2-adrenergic receptor , 1996, Nature.

[82]  R. Schekman,et al.  A test of clathrin function in protein secretion and cell growth. , 1985, Science.

[83]  A. Podtelejnikov,et al.  Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid , 1999, Nature.

[84]  F. Wieland,et al.  Mechanisms of vesicle formation: insights from the COP system. , 1999, Current opinion in cell biology.

[85]  J. Rothman,et al.  Megavesicles Implicated in the Rapid Transport of Intracisternal Aggregates across the Golgi Stack , 2000, Cell.

[86]  J. Heuser.  Three-dimensional visualization of coated vesicle formation in fibroblasts , 1980, The Journal of cell biology.

[87]  W. Balch,et al.  AP-2-containing clathrin coats assemble on mature lysosomes , 1996, The Journal of cell biology.

[88]  J C Olivo,et al.  Dual-color visualization of trans-Golgi network to plasma membrane traffic along microtubules in living cells. , 1999, Journal of cell science.

[89]  S. Harrison,et al.  Atomic Structure of Clathrin A β Propeller Terminal Domain Joins an α Zigzag Linker , 1998, Cell.

[90]  B. Pearse.  Coated vesicles from pig brain: purification and biochemical characterization. , 1975, Journal of molecular biology.

[91]  P. Chavrier,et al.  ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[92]  Pier Paolo Di Fiore,et al.  Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis , 1998, Nature.

[93]  D. Fremont,et al.  Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[94]  M. Roth,et al.  Phosphatidic acid formation by phospholipase D is required for transport from the endoplasmic reticulum to the Golgi complex , 1997, Current Biology.

[95]  Roman S. Polishchuk,et al.  Correlative Light-Electron Microscopy Reveals the Tubular-Saccular Ultrastructure of Carriers Operating between Golgi Apparatus and Plasma Membrane , 2000, The Journal of cell biology.

[96]  J. Bonifacino,et al.  The Medium Subunits of Adaptor Complexes Recognize Distinct but Overlapping Sets of Tyrosine-based Sorting Signals* , 1998, The Journal of Biological Chemistry.

[97]  I. Mellman,et al.  Sorting out adaptors. , 1998, Biochimica et biophysica acta.

[98]  J. Lippincott-Schwartz,et al.  Golgi membrane dynamics. , 1998, Molecular biology of the cell.

[99]  R. Kelly,et al.  Selective Inhibition of Adaptor Complex‐Mediated Vesiculation , 2000, Traffic.

[100]  P. Sternberg,et al.  unc-101, a gene required for many aspects of Caenorhabditis elegans development and behavior, encodes a clathrin-associated protein. , 1994, Genes & Development.