Confirmation from choline acetylase analyses of a massive cholinergic innervation to the rat hippocampus

1. Unilateral lesions were made in the fimbria of adult rats with a stereotaxically‐applied, radio‐frequency current. After 3‐23 days survival, fresh, unfixed, transverse sections were cut of the forebrain. Half the sections were stained histochemically to show the distribution of acetylcholinesterase (AChE) activity. From the other sections, small, anatomically defined areas were dissected out and assayed for choline acetylase (ChAc) activity (acetyl‐CoA: choline‐O‐acetyltransferase EC2.3.1.6).

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