Automated System for Cell Manipulation and Rotation

Cell manipulation is an important step for microsurgical operations to extract/inject material from/within the cell. Cell manipulation may include translation and/or rotating cell to a desired position and orientation in preparing it for the next step of microsurgery. Extraction of cellular components is an essential aspect of cell surgery. The extraction must be carried out without adverse effects on the cell. The cellular material must be extracted from the desired location in the cell according to the cell type. Hence, the cell first must be manipulated and rotated precisely. The success of manual cell rotation approaches currently depends on the operator skill, which may vary over the time and from one operator to another one. Additionally, low efficiency, low repeatability and low controllability negatively impact on the process. In this article, an automatic cell rotation and manipulation system is presented, in which we propose the use of conventional tools and techniques that are in use in clinical labs now. The system will replace the manual manipulation procedure, to increase the microsurgical operations efficiency without the introduction of costly new tools and time. The proposed rotation system is efficient, simple and cost-effective. The system uses visual feedback control to rotate and visually track the cell orientation. The system can rotate the cell in the in-plane and out of plane directions. The simulation results show excellent potential for the system since it can reorient the cell with an error margin of less than 5°. The mouse embryo at the blastocyst stage is used for the system validation.

[1]  U. Zimmermann,et al.  Rotation of a single cell in a discontinuous rotating electric field , 1983 .

[2]  H. Rubinsztein-Dunlop,et al.  Optical alignment and spinning of laser-trapped microscopic particles , 1998, Nature.

[3]  Yongqiang Qiu,et al.  Letters: optically transparent piezoelectric transducer for ultrasonic particle manipulation , 2014, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control.

[4]  I-Kao Chiang,et al.  On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves , 2012, Proceedings of the National Academy of Sciences.

[5]  Jovitha Jerome,et al.  Algebraic approach for selecting the weighting matrices of linear quadratic regulator , 2014, 2014 International Conference on Green Computing Communication and Electrical Engineering (ICGCCEE).

[6]  J. Voldman,et al.  Dielectrophoretic registration of living cells to a microelectrode array. , 2004, Biosensors & bioelectronics.

[7]  D. Vezenov,et al.  Dielectrophoretic tweezers as a platform for molecular force spectroscopy in a highly parallel format , 2011, 2012 38th Annual Northeast Bioengineering Conference (NEBEC).

[8]  D. Grier.  A revolution in optical manipulation , 2003, Nature.

[9]  B Wagner,et al.  Levitation, holding, and rotation of cells within traps made by high-frequency fields. , 1992, Biochimica et biophysica acta.

[10]  J. Hierrezuelo,et al.  Sliding and rolling: the physics of a rolling ball , 1995 .

[11]  Win Tun Latt,et al.  Visual Servoed Three-Dimensional Cell Rotation System , 2015, IEEE Transactions on Biomedical Engineering.

[12]  Chen Feng,et al.  A fully automated robotic system for three-dimensional cell rotation , 2016, 2016 IEEE International Conference on Robotics and Automation (ICRA).

[13]  Kishan Dholakia,et al.  Optoelectronic tweezers , 2005, Nature materials.

[14]  S. Amir Ghoreishi,et al.  Optimal Weighting Matrices Design for LQR Controller Based on Genetic Algorithm and PSO , 2012 .

[15]  Y. Matsumoto.  High Aspect Ratio Sloping and Curved Structures Fabricated by Proximity and UV-LED Backside Exposure , 2010 .

[16]  Cees Dekker,et al.  Recent advances in magnetic tweezers. , 2012, Annual review of biophysics.

[17]  W. Sawyer,et al.  Macroscopic Friction Coefficient Measurements on Living Endothelial Cells , 2007 .

[18]  Peter C. Y. Chen,et al.  Force Sensing and Control in Micromanipulation , 2006, IEEE Transactions on Systems, Man, and Cybernetics, Part C (Applications and Reviews).

[19]  G. Batchelor,et al.  An Introduction to Fluid Dynamics , 1968 .

[20]  James K. Mills,et al.  Development of a cell orientation control system for mouse embryo using electro-rotation , 2014, 2014 IEEE International Conference on Mechatronics and Automation.

[21]  Satyandra K. Gupta,et al.  Optical Tweezers: Autonomous Robots for the Manipulation of Biological Cells , 2014, IEEE Robotics & Automation Magazine.

[22]  V. Shastri,et al.  Mechanically tailored agarose hydrogels through molecular alloying with β-sheet polysaccharides. , 2015, Macromolecular rapid communications.

[23]  Yu Sun,et al.  Three-Dimensional Rotation of Mouse Embryos , 2012, IEEE Transactions on Biomedical Engineering.

[24]  L. Oddershede,et al.  Optical Tweezers Cause Physiological Damage to Escherichia coli and Listeria Bacteria , 2008, Applied and Environmental Microbiology.