Multivalent peptide and protein dendrimers using native chemical ligation.

A wide variety of well-defined multivalent peptides and proteins can be made by conjugating synthetic peptides and recombinantly expressed proteins to cysteine-functionalized dendrimers using native chemical ligation (see picture). This modular approach provides access to dendrimers that are attractive both for understanding fundamental issues of multivalency in biological interactions as well as for biomedical applications.

[1]  P. Heegaard,et al.  Dendrimers in drug research. , 2004, Chemical Society reviews.

[2]  Julio A Camarero,et al.  Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for "protein chip" fabrication. , 2004, Journal of the American Chemical Society.

[3]  S. Yao,et al.  Expanded utility of the native chemical ligation reaction. , 2004, Chemistry.

[4]  A. Heck,et al.  Native protein mass spectrometry: from intact oligomers to functional machineries. , 2004, Current opinion in chemical biology.

[5]  Albert J R Heck,et al.  Investigation of intact protein complexes by mass spectrometry. , 2004, Mass spectrometry reviews.

[6]  A. Beck‐Sickinger,et al.  Expressed protein ligation. Method and applications. , 2004, European journal of biochemistry.

[7]  Min Zhou,et al.  Helical supramolecules and fibers utilizing leucine zipper-displaying dendrimers. , 2004, Journal of the American Chemical Society.

[8]  Xiaoqiang Shen,et al.  Catalytic asymmetric acyl halide-aldehyde cyclocondensation reactions of substituted ketenes. , 2004, Journal of the American Chemical Society.

[9]  Yi Hu,et al.  Versatile protein biotinylation strategies for potential high-throughput proteomics. , 2004, Journal of the American Chemical Society.

[10]  R. Kluger,et al.  Hemoglobin dendrimers: functional protein clusters. , 2003, Journal of the American Chemical Society.

[11]  H. Ploegh,et al.  Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. , 2002, Chemistry & biology.

[12]  J Fraser Stoddart,et al.  Design and synthesis of glycodendrimers. , 2002, Journal of biotechnology.

[13]  J. Tam,et al.  Peptide dendrimers: applications and synthesis. , 2002, Journal of biotechnology.

[14]  R. Liskamp,et al.  Synthesis of Lactose Dendrimers and Multivalency Effects in Binding to the Cholera Toxin B Subunit , 2001 .

[15]  L. Kiessling,et al.  Synthetic multivalent ligands in the exploration of cell-surface interactions. , 2000, Current opinion in chemical biology.

[16]  Chi‐Huey Wong,et al.  Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes , 2000 .

[17]  W. Hol,et al.  High-Affinity Pentavalent Ligands of Escherichia coli Heat-Labile Enterotoxin by Modular Structure-Based Design , 2000 .

[18]  R. Read,et al.  Shiga-like toxins are neutralized by tailored multivalent carbohydrate ligands , 2000, Nature.

[19]  J. Mitchell,et al.  A direct method for the formation of peptide and carbohydrate dendrimers. , 1999, Bioorganic & medicinal chemistry letters.

[20]  J. Griffin,et al.  Protein synthesis by native chemical ligation: expanded scope by using straightforward methodology. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[21]  E. W. Meijer,et al.  About Dendrimers: Structure, Physical Properties, and Applications. , 1999, Chemical reviews.

[22]  J. Baker,et al.  Inhibition of viral adhesion and infection by sialic-acid-conjugated dendritic polymers. , 1999, Bioconjugate chemistry.

[23]  George M Whitesides,et al.  Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors. , 1998, Angewandte Chemie.

[24]  G. M. Whitesides,et al.  Polyvalente Wechselwirkungen in biologischen Systemen: Auswirkungen auf das Design und die Verwendung multivalenter Liganden und Inhibitoren , 1998 .

[25]  T. Muir,et al.  Expressed protein ligation: a general method for protein engineering. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[26]  G M Whitesides,et al.  A trivalent system from vancomycin.D-ala-D-Ala with higher affinity than avidin.biotin. , 1998, Science.

[27]  J. Tam,et al.  Synthesis of Peptide Dendrimer , 1994 .

[28]  H. Mihara,et al.  Construction of α-helical peptide dendrimers conjugated with multi- metalloporphyrins: photoinduced electron transfer on dendrimer architecture , 2000 .