Effect of solvent (CO2/ethanol/H2O) on the fractionated enhanced solvent extraction of anthocyanins from elderberry pomace

Abstract The management of agro-industrial residues is an important issue for environmental reasons and the reuse of byproducts represents a good alternative, especially if it is conjugated with green technologies and the production of valuable products. Portuguese elderberry pomace is rich in anthocyanins with therapeutic properties that confer to this byproduct potential to be applied in the food and pharmaceutical industries. Fractionated high pressure extractions from elderberry pomace were performed using supercritical CO 2 extraction, followed by enhanced solvent extraction (ESE) with diverse CO 2 /ethanol/H 2 O solvent mixtures (0–90%, 0.5–100%, 0–95%, v/v/v), at 313 K and 21 MPa, in order to obtain anthocyanin-rich fractions. The ESE solvent mixtures had a substantial effect on extracts yield and composition. The maximum extraction yield (24.2%), total phenolic compounds (15.8% gallic acid equivalents), total flavonoids (8.9% epicatechin equivalents), total anthocyanins (15.0% cyanidin-3-glucoside equivalents) and antioxidant activity (IC 50 of 21 μg) achieved highlight the great potential of elderberry pomace for valuable applications.

[1]  A. Evidente,et al.  Isolation, spectroscopy and selective phytotoxic effects of polyphenols from vegetable waste waters , 1992 .

[2]  P. Mauri,et al.  Separation of flavonol-2-O-glycosides from Calendula officinalis and Sambucus nigra by high-performance liquid and micellar electrokinetic capillary chromatography. , 1992, Journal of chromatography.

[3]  Jungmin Lee,et al.  Correlation of two anthocyanin quantification methods: HPLC and spectrophotometric methods , 2008 .

[4]  L. Cheung,et al.  Antioxidant activity and total phenolics of edible mushroom extracts , 2003 .

[5]  C. Rice-Evans,et al.  Structure-antioxidant activity relationships of flavonoids and phenolic acids. , 1996, Free radical biology & medicine.

[6]  N. Seeram.  Berry fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease. , 2008, Journal of agricultural and food chemistry.

[7]  Kazuki Saito,et al.  LC/PDA/ESI-MS Profiling and Radical Scavenging Activity of Anthocyanins in Various Berries , 2004, Journal of biomedicine & biotechnology.

[8]  I. Zizović,et al.  Swelling of plant material in supercritical carbon dioxide , 2010 .

[9]  Ronald E. Wrolstad,et al.  Current Protocols in Food Analytical Chemistry , 2000 .

[10]  F. J. Francis,et al.  SOLVENT EXTRACTION OF ANTHOCYANINS FROM WINE POMACE , 1980 .

[11]  M. Pinelo,et al.  Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. , 2005, Journal of agricultural and food chemistry.

[12]  M. S. Blois,et al.  Antioxidant Determinations by the Use of a Stable Free Radical , 1958, Nature.

[13]  Mengcheng Tang,et al.  The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals , 1999 .

[14]  N. Turker,et al.  Effects of pH and temperature of extraction medium on effective diffusion coefficient of anthocynanin pigments of black carrot (Daucus carota var. L.) , 2006 .

[15]  Curtis L. Weller,et al.  Recent advances in extraction of nutraceuticals from plants , 2006 .

[16]  Eric J. Beckman,et al.  Enzyme Activity in Supercritical Fluids , 1995 .

[17]  O. Catchpole,et al.  Measurement and modelling of the ternary phase equilibria for high pressure carbon dioxide-ethanol-water mixtures , 2007 .

[18]  P. Butz,et al.  Extraction of anthocyanins from grape skins assisted by high hydrostatic pressure , 2009 .

[19]  Jungmin Lee,et al.  Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. , 2005, Journal of AOAC International.

[20]  Herminia Domínguez,et al.  Supercritical CO2 extraction and purification of compounds with antioxidant activity. , 2006, Journal of agricultural and food chemistry.

[21]  C. Wai,et al.  pH-Defining Equilibrium between Water and Supercritical CO2. Influence on SFE of Organics and Metal Chelates , 1995 .

[22]  Florian C. Stintzing,et al.  Functional properties of anthocyanins and betalains in plants, food, and in human nutrition , 2004 .

[23]  J. Schultz,et al.  LIMITATIONS OF FOLIN ASSAYS OF FOLIAR PHENOLICS IN ECOLOGICAL STUDIES , 2001, Journal of Chemical Ecology.

[24]  C. Mantell,et al.  Kinetics and Mathematical Modeling of Anthocyanin Extraction with Carbon Dioxide and Methanol at High Pressure , 2003 .

[25]  R. Fink,et al.  Elderberry flavonoids bind to and prevent H1N1 infection in vitro. , 2009, Phytochemistry.

[26]  Ross R. Weikel,et al.  Reversible in situ acid formation for β-pinene hydrolysis using CO2 expanded liquid and hot water , 2004 .

[27]  D. Stewart,et al.  Anthocyanin-flavanol condensation products from black currant (Ribes nigrum L.). , 2005, Journal of agricultural and food chemistry.

[28]  Jungmin Lee,et al.  Anthocyanins and other polyphenolics in American elderberry (Sambucus canadensis) and European elderberry (S. nigra) cultivars. , 2007, Journal of the science of food and agriculture.

[29]  C. Eckert,et al.  In Situ Formation of Alkylcarbonic Acids with CO2 , 2001 .

[30]  Ramona Lichtenthäler,et al.  Total oxidant scavenging capacities of common European fruit and vegetable juices. , 2005, Journal of agricultural and food chemistry.

[31]  J. Flink,et al.  Anthocyanin colourants from elderberry (Sambucus nigra L.). 1. Process considerations for production of the liquid extract , 2007 .

[32]  A. Lugasi,et al.  Antioxidant properties of commercial alcoholic and nonalcoholic beverages. , 2003, Die Nahrung.

[33]  Ž. Knez,et al.  Extraction of phenolic compounds from elder berry and different grape marc varieties using organic solvents and/or supercritical carbon dioxide , 2009 .

[34]  V. L. Singleton,et al.  Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents , 1965, American Journal of Enology and Viticulture.

[35]  M. Castellari,et al.  Effects of high pressure processing on polyphenoloxidase enzyme activity of grape musts , 1997 .

[36]  I. J. Seabra,et al.  Fractioned High Pressure Extraction of Anthocyanins from Elderberry (Sambucus nigra L.) Pomace , 2010 .

[37]  R. Carle,et al.  Color and antioxidant properties of cyanidin-based anthocyanin pigments. , 2002, Journal of agricultural and food chemistry.