The Effect of Cement and wrapping on the Decomposition rate of the Rabbit Carcasses

Taphonomic study was fi rst time conducted in Kuala Lumpur, capital city of Malaysia. In this research model, male Oryctolagus cunicullus weighed 1.8-2.6 kg each were killed by Dolethal intravenously at the ear region. In Phase 1 study, eight subjects were wrapped in a sack and cemented within a container to be compared with the other four controls at each location labelled A (ground) and B (manmade freshwater pond). Phase 2 involved duplicating set of 15 cemented samples comparable to 1 control made up to total of 32 subjects which were all put into a compact polytank containing freshwater to simulate a case study. Taphonomic changes were observed and scored using TBS system incorporating of fresh stage, early decomposition stage, advanced decomposition stage and skeletonisation stage. The ambient temperature of surroundings with 28.81C±4.21°C and 29.21°C±4.57°C (mean±S.D.) while relative humidity of air with 74.49 %±14.61% and 79.15%±16.32% (mean±S.D.) were recorded for Phase 1 and Phase 2 study respectively. Time taken for ground control exposed and wrapped carcasses to reach fi rst sign of skeletonised stage were four days and fi ve days respectively. Whilst freshwater controls reached initial skeletonised stage within one week for exposed carcass and two weeks for wrapped carcass. Within the control and cemented sample carcasses, TBS scores increased from initial stages of decomposition and become plateau after advanced decomposition. The cementing factor have superseded the wrapping factor due to its stronger physical barrier effect to slow down the decomposition more than half compared to controls based on Multiple way ANOVA test. Phase 2 study has demonstrated more accurately on the decomposition rate of the cemented samples. It showed that wrapping and cementing factors have delayed the decomposition process of the rabbit carcasses about 4 times to reach the initial stage of skeletonisation compared to the control carcasses. There was absence of insect activity within the cemented samples, hence the microorganism activity would be the only contributor to the decaying process within the cemented samples at slower rate comparing to the exposed or wrapped controls. Research Article The Effect of Cement and wrapping on the Decomposition rate of the Rabbit Carcasses Lai Poh Soon1*, Khoo Lay See1, Nazni Wasi Ahmad2, Azlina Zulkapli3, Nurul Kharmila Abdullah4, Mohd Shah Mahmood5 and Ahmad Hafi zam Hasmi5 1Forensic Scientifi c Offi cer of Institute of Forensic Medicine Malaysia, Kuala Lumpur Hospital, Malaysia 2Forensic Entomologist, Unit of Entomology, Institute of Medical Research, Malaysia 3Veterinary Offi cer, Unit of Animal Care, Institute of Medical Research, Malaysia 4Senior Lecturer and Forensic Pathologist, University of Science Islamic Malaysia, Kuala Lumpur Hospital, Malaysia 5Forensic Pathologist, Institute of Forensic Medicine Malaysia, Kuala Lumpur Hospital, Malaysia *Address for Correspondence: Dr. Lai Poh Soon, Forensic Scientifi c Offi cer of Institute of Forensic Medicine Malaysia, Kuala Lumpur Hospital, Malaysia; Tel: +603 2690 6694, Fax: +603 2694 1422; Email: roysonn1@hotmail.com Submitted: 08 August 2017 Approved: 15 August 2017 Published: 16 August 2017 Copyright: 2017 Soon LP, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

[1]  G. Hamilton,et al.  Death eaters respond to the dark mark of decomposition day and night: observations of initial insect activity on piglet carcasses , 2016, International Journal of Legal Medicine.

[2]  J. Brooks,et al.  Postmortem Changes in Animal Carcasses and Estimation of the Postmortem Interval , 2016, Veterinary pathology.

[3]  S. Tafuri,et al.  Bodies in sequestered and non-sequestered aquatic environments: a comparative taphonomic study using decompositional scoring system. , 2014, Science & justice : journal of the Forensic Science Society.

[4]  Moray Newlands,et al.  Suitability of cement combinations for carbonation resistance of structural concrete , 2014 .

[5]  M. Steyn,et al.  The effect of body size on the rate of decomposition in a temperate region of South Africa. , 2013, Forensic science international.

[6]  S. Forbes,et al.  Human Versus Animal: Contrasting Decomposition Dynamics of Mammalian Analogues in Experimental Taphonomy , 2013, Journal of forensic sciences.

[7]  E. Cunha,et al.  Can we infer post mortem interval on the basis of decomposition rate? A case from a Portuguese cemetery. , 2013, Forensic science international.

[8]  S. Andreola,et al.  Burial of Piglet Carcasses in Cement: A Study of Macroscopic and Microscopic Alterations on an Animal Model , 2013, The American journal of forensic medicine and pathology.

[9]  E. Panacek,et al.  Comparison of Protocols for Measuring and Calculating Postmortem Submersion Intervals for Human Analogs in Fresh Water , 2013, Journal of forensic sciences.

[10]  J. Denton,et al.  Effects of hydrated lime and quicklime on the decay of buried human remains using pig cadavers as human body analogues. , 2012, Forensic science international.

[11]  Sasha C. Voss,et al.  Decomposition and insect succession of clothed and unclothed carcasses in Western Australia. , 2011, Forensic science international.

[12]  R. Byard,et al.  Factors and processes causing accelerated decomposition in human cadavers - An overview. , 2011, Journal of forensic and legal medicine.

[13]  S. Matuszewski,et al.  Insect succession and carrion decomposition in selected forests of Central Europe. Part 1: Pattern and rate of decomposition. , 2010, Forensic science international.

[14]  C. Moffatt,et al.  Debugging Decomposition Data—Comparative Taphonomic Studies and the Influence of Insects and Carcass Size on Decomposition Rate , 2010, Journal of forensic sciences.

[15]  G. Anderson,et al.  The Influence of Clothing and Wrapping on Carcass Decomposition and Arthropod Succession During the Warmer Seasons in Central South Africa * , 2009, Journal of forensic sciences.

[16]  Carly Marie Shattuck An Analysis of Decomposition Rates on Outdoor Surface Variations in Central Texas , 2009 .

[17]  James R. Miller,et al.  Examination of Nocturnal Blow Fly (Diptera: Calliphoridae) Oviposition on Pig Carcasses in Mid-Michigan , 2009, Journal of medical entomology.

[18]  A. Abu Hassan,et al.  A preliminary study on the decomposition and dipteran associated with exposed carcasses in an oil palm plantation in Bandar Baharu, Kedah, Malaysia. , 2009, Tropical biomedicine.

[19]  J. R. Pujol-Luz,et al.  Coprophanaeus lancifer (Linnaeus, 1767) (Coleoptera, Scarabaeidae) activity moves a man-size pig carcass: relevant data for forensic taphonomy. , 2008, Forensic Science International.

[20]  David O. Carter,et al.  The biochemical alteration of soil beneath a decomposing carcass. , 2008, Forensic science international.

[21]  A. Mohamad,et al.  Insect succession on a decomposing piglet carcass placed in a man-made freshwater pond in Malaysia. , 2008, Tropical biomedicine.

[22]  J. Jeffery,et al.  A preliminary study of insect succession on a pig carcass in a palm oil plantation in Malaysia. , 2007, Tropical biomedicine.

[23]  Heather A. Thew EFFECTS OF LIME ON THE DECOMPOSITION RATE OF BURIED REMAINS , 2006 .

[24]  K. Pueschel,et al.  Information is Everything - A Case Report Demonstrating the Necessity of Entomological Knowledge at the Crime Scene , 2004 .

[25]  G. Di Vella,et al.  Factors affecting decomposition and Diptera colonization. , 2001, Forensic science international.

[26]  M. Sorg,et al.  Advances in Forensic Taphonomy : Method, Theory, and Archaeological Perspectives , 2001 .

[27]  L. Sponsel Fundamentals of human ecology , 1999 .

[28]  R W Mann,et al.  Time since death and decomposition of the human body: variables and observations in case and experimental field studies. , 1990, Journal of forensic sciences.

[29]  R. E. Williams,et al.  Use of aquatic insects in determining submersion interval. , 1989, Journal of forensic sciences.

[30]  D. Gifford Taphonomy and Paleoecology: A Critical Review of Archaeology's Sister Disciplines , 1981 .