Effect of different freezing and storage condition on the physical properties of protein coagulum (Firm Tofu)

Abstract This article attempted to apply supercooling freezing under atmospheric pressure to minimize the freezing damage on firm (momen) tofu, which is a representative of agglomeration type protein coagulum. Especially, we focused on the influence of different freezing rate after the releasing from supercooling state. Storage test up to one month with different storage temperature was also carried out. The quality parameters such as drip loss, texture, and microstructure were analyzed and compared with conventional methods (slow freezing and rapid freezing) after the storage under −80 °C and -20 °C up to one month. As the results, significant advantages were confirmed on the sample prepared by supercooling freezing even after the one-month storage. Further, higher freezing rate after the releasing from supercooling states successfully reduced the drip loss and kept good texture, with less microstructural damage. The possible mechanism on which supercooling freezing affects ice crystals formation and quality degradation are thoroughly discussed.

[1]  T. Ono,et al.  Incorporation of soy milk lipid into protein coagulum by addition of calcium chloride. , 1999, Journal of agricultural and food chemistry.

[2]  Manabu Watanabe,et al.  Effects of freezing on microstructure and rehydration properties of freeze-dried soybean curd , 2016 .

[3]  Koji Iwamura,et al.  A Study on Supercooled Storage of Leaf Lettuces Produced in Plant Factory , 2017 .

[4]  Young-Boong Kim,et al.  Changes in Ultrastructure and Sensory Characteristics on Electro-magnetic and Air Blast Freezing of Beef during Frozen Storage , 2015, Korean journal for food science of animal resources.

[5]  A. Myerson.  Handbook of Industrial Crystallization , 2002 .

[6]  Tokuji Watanabe,et al.  Denaturation of Soybean Protein by Freezing Part I , 1971 .

[7]  Juhee Ahn,et al.  Effect of isothiocyanates from horseradish (Armoracia rusticana) on the quality and shelf life of tofu , 2010 .

[8]  N. Ogawa,et al.  Structural and Textural Quality of Kinu‐Tofu Frozen‐then‐Thawed at High‐Pressure , 2006 .

[9]  L. Otero,et al.  High-pressure-shift freezing: Main factors implied in the phase transition time , 2006 .

[10]  Manabu Watanabe,et al.  Effect of the Breaking Temperature of Supercooling on Ice Characteristics and Drip Loss of Foods in Supercooled Freezing Method , 2014 .

[11]  Manabu Watanabe,et al.  The effect of supercooling on ice structure in tuna meat observed by using X-ray computed tomography , 2015 .

[12]  A. Delgado,et al.  Microstructural changes in strawberry after freezing and thawing processes , 2005 .

[13]  J. Lucey,et al.  Formation and physical properties of yogurt. , 2010 .

[14]  H. R. Ashraf,et al.  Microbiological survey of tofu sold in a rural Illinois county. , 1999, Journal of food protection.

[15]  S. Kaur,et al.  PROTEIN ISOLATES : PRODUCTION, FUNCTIONAL PROPERTIES AND APPLICATION - , 2014 .

[16]  Jin-Woong Jeong,et al.  Temperature Changes during Freezing and Effect of Physicochemical Properties after Thawing on Meat by Air Blast and Magnetic Resonance Quick Freezing , 2013 .

[17]  Christian James,et al.  The freezing and supercooling of garlic (Allium sativum L.) , 2009 .

[18]  B. Simpson,et al.  Effect of freezing conditions and storage on ice crystal and drip volume in turbot (Scophthalmus maximus) , 2000 .

[19]  Da-Wen Sun,et al.  Heat and mass transfer models for predicting freezing processes – a review , 2001 .

[20]  Bing Li,et al.  Novel methods for rapid freezing and thawing of foods - a review , 2002 .

[21]  Y. Hui,et al.  Handbook of Frozen Foods , 2007 .

[22]  Johanna Söderberg.  Functional properties of legume proteins compared to egg proteins and their potential as egg replacers in vegan food , 2013 .

[23]  V. Obatolu.  Effect of different coagulants on yield and quality of tofu from soymilk , 2008 .

[24]  S. Charoenrein,et al.  Effect of freezing rates and freeze-thaw cycles on the texture, microstructure and pectic substances of mango. , 2016 .

[25]  Judith Evans,et al.  The use of supercooling for fresh foods: A review , 2015 .

[26]  J. Meullenet,et al.  Changes in broiler breast fillet tenderness, water-holding capacity, and color attributes during long-term frozen storage. , 2008, Journal of food science.

[27]  Da-Wen Sun,et al.  Innovative applications of power ultrasound during food freezing processes—a review , 2006 .

[28]  P. Fox,et al.  Fundamentals of Cheese Science , 2000 .

[29]  M. A. Pal,et al.  Paneer production: A review , 2011, Journal of food science and technology.

[30]  T. S. Nordtvedt,et al.  Changes in water holding capacity and drip loss of Atlantic salmon (Salmo salar) muscle during superchilled storage , 2014 .

[31]  A. Mustafa,et al.  Effects of freezing on composition and fatty acid profiles of sheep milk and cheese , 2006 .

[32]  W. J. Dyer,et al.  Temperature and Deteriorative Changes in Postrigor Cod Muscle Stored up to 14 Days in the Superchill Range, −1 to −4 C , 1975 .

[33]  H. Ramaswamy,et al.  Effect of high-pressure versus conventional thawing on color, drip loss and texture of Atlantic salmon frozen by different methods , 2004 .

[34]  M. Havet,et al.  Effect of static electric field on ice crystal size reduction during freezing of pork meat , 2013 .

[35]  L. Otero,et al.  Preservation of Microstructure in Peach and Mango during High-pressure-shift Freezing , 2000 .

[36]  Songming Zhu,et al.  Pressure Shift Freezing of Pork Muscle: Effect on Color, Drip Loss, Texture, and Protein Stability , 2004, Biotechnology progress.

[37]  Yangzi Xu,et al.  Effect of freeze-thaw treatment on the structure and texture of soft and firm tofu , 2016 .