Video tracking algorithm of long-term experiment using stand-alone recording system.

Many medical and behavioral applications require the ability to monitor and quantify the behavior of small animals. In general these animals are confined in small cages. Often these situations involve very large numbers of cages. Modern research facilities commonly monitor simultaneously thousands of animals over long periods of time. However, conventional systems require one personal computer per monitoring platform, which is too complex, expensive, and increases power consumption for large laboratory applications. This paper presents a simplified video tracking algorithm for long-term recording using a stand-alone system. The feature of the presented tracking algorithm revealed that computation speed is very fast data storage requirements are small, and hardware requirements are minimal. The stand-alone system automatically performs tracking and saving acquired data to a secure digital card. The proposed system is designed for video collected at a 640 x 480 pixel with 16 bit color resolution. The tracking result is updated every 30 frames/s. Only the locomotive data are stored. Therefore, the data storage requirements could be minimized. In addition, detection via the designed algorithm uses the Cb and Cr values of a colored marker affixed to the target to define the tracked position and allows multiobject tracking against complex backgrounds. Preliminary experiment showed that such tracking information stored by the portable and stand-alone system could provide comprehensive information on the animal's activity.

[1]  Charles F. Babbs,et al.  A novel open field activity detector to determine spatial and temporal movement of laboratory animals after injury and disease , 2006, Journal of Neuroscience Methods.

[2]  Hans-Peter Lipp,et al.  Extended analysis of path data from mutant mice using the public domain software Wintrack , 2001, Physiology & Behavior.

[3]  A. J Spink,et al.  The EthoVision video tracking system—A tool for behavioral phenotyping of transgenic mice , 2001, Physiology & Behavior.

[4]  M. Grossmann,et al.  A simple computer based system to analyze Morris water maze trials on-line , 1996, Journal of Neuroscience Methods.

[5]  L P Noldus,et al.  EthoVision: A versatile video tracking system for automation of behavioral experiments , 2001, Behavior research methods, instruments, & computers : a journal of the Psychonomic Society, Inc.

[6]  T. Ono,et al.  A combined electrophysiological and video data acquisition system using a single computer , 1999, Journal of Neuroscience Methods.

[7]  Thomas A. Cleland,et al.  Inexpensive ethography using digital video , 2003, Journal of Neuroscience Methods.

[8]  Lucas P. J. J. Noldus,et al.  Computerised video tracking, movement analysis and behaviour recognition in insects , 2002 .

[9]  N S Zefirov,et al.  Versatile computerized system for tracking and analysis of water maze tests , 2001, Behavior research methods, instruments, & computers : a journal of the Psychonomic Society, Inc.

[10]  Leonie de Visser,et al.  Automated home cage observations as a tool to measure the effects of wheel running on cage floor locomotion , 2005, Behavioural Brain Research.

[11]  Paulo Aguiar,et al.  OpenControl: A free opensource software for video tracking and automated control of behavioral mazes , 2007, Journal of Neuroscience Methods.

[12]  Ming-Shing Young,et al.  Integrated digital image and accelerometer measurements of rat locomotor and vibratory behaviour , 2007, Journal of Neuroscience Methods.

[13]  F. K. Lam,et al.  A novel system for simultaneous monitoring of locomotor and sound activities in animals , 2000, Journal of Neuroscience Methods.