Sensitivity and detection limit of concentration and adsorption measurements by laser-induced surface-plasmon resonance.

The shot noise limitation as well as other factors that influence the sensitivity of measurements with a surface plasmon resonance (SPR) sensor are considered. It is demonstrated that minute changes in the refractive index of a medium close to the surface of a metal film can be detected owing to a shift in the resonance angle. In particular, changes in the adsorption layer of only a fraction of a biomolecular monolayer could be measured. Data for SPR are presented with adjacent media of air, water, as well as aqueous solutions of ethanol and sodium chloride at different concentrations. The immobilization of the protein bovine serum albumin to a specially prepared surface was monitored with the SPR technique. Specific responses to changes in the concentration and thickness of the adsorption layer were determined. The angular resolution of the present apparatus is approximately 1 millidegree, corresponding to a detection limit for an adsorbed protein layer of 15 pg/mm(2), which is still 2 to 3 orders of magnitude larger than the shot-noise limit, and therefore a further improvement in sensitivity is possible.

[1]  Kolomenskii,et al.  Nonlinear excitation of capillary waves by the Marangoni motion induced with a modulated laser beam. , 1995, Physical review. B, Condensed matter.

[2]  P. Gershon,et al.  Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detector. , 1995, Journal of immunological methods.

[3]  S Kawata,et al.  Surface-plasmon sensor for absorption-sensitivity enhancement. , 1994, Applied optics.

[4]  G. Dougherty,et al.  A compact optoelectronic instrument with a disposable sensor based on surface plasmon resonance , 1993 .

[5]  F. F. Mul.  Monte Carlo simulations of laser Doppler blood-flow measurements in tissue: reply to comment. , 1992 .

[6]  H. E. de Bruijn,et al.  Choice of metal and wavelength for surface-plasmon resonance sensors: some considerations. , 1992, Applied optics.

[7]  R. Karlsson,et al.  Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology. , 1991, BioTechniques.

[8]  S. Loefas,et al.  Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. , 1991, Analytical biochemistry.

[9]  B. Persson,et al.  Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins , 1991 .

[10]  S. Shiokawa,et al.  Interactions of surface plasmons with surface acoustic waves and the study of the properties of Ag films , 1991 .

[11]  Jan Greve,et al.  Vibrating mirror surface plasmon resonance immunosensor , 1991 .

[12]  D. Reinhoudt,et al.  Optimization of a chemooptical surface plasmon resonance based sensor. , 1990, Applied optics.

[13]  S. Kawata,et al.  Optical chemical sensor based on surface plasmon measurement. , 1988, Applied optics.

[14]  Jan Greve,et al.  Surface plasmon resonance immunosensors: sensitivity considerations , 1988 .

[15]  D. Sarid,et al.  Determination of loss coefficients of long-range surface plasmons. , 1986, Applied optics.

[16]  E. Arakawa,et al.  Photoacoustic observation of nonradiative decay of surface plasmons in silver , 1981 .

[17]  E. Kretschmann.  Die Bestimmung optischer Konstanten von Metallen durch Anregung von Oberflächenplasmaschwingungen , 1971 .