The complete mitochondrial genomes of two weevils, Eucryptorrhynchus chinensis and E. brandti: conserved genome arrangement in Curculionidae and deficiency of tRNA-Ile gene

Abstract The weevils Eucryptorrhynchus chinensis and Eucryptorrhynchus brandti (Coleoptera: Curculionidae), are two of the most important pests of the tree-of-heaven, Ailanthus altissima, which is found throughout China. In this study, the complete mitogenomes of the two weevils have been sequenced using Illumina HiSeqTM 2000. The mitogenomes of E. chinensis and E. brandti are 15,628bp and 15,597bp long with A+T contents of 77.7% and 76.6%, respectively. Both species have typical circular mitochondrial genomes that encode 36 genes. Except the deficiency of tRNA-Ile, the gene composition and order of E. chinensis and E. brandti are identical to the inferred ancestral gene arrangement of insects. In both mitochondrial genomes, the start codons for COI and ND1 are AAT and TTG, respectively. A5bp motif (TACTA) is detected in intergenic region between the tRNA-Ser (UCN) and ND1 genes. The ATP8/ATP6 and ND4L/ND4 gene pairs appear to overlap four or seven nucleotides (ATAA/ATGATAA) in different reading frames. The complete sequences of AT-rich region have two regions including tandem repeats. The study identifies useful genetic markers for studying the population genetics, molecular identification and phylogeographics of Eucryptorrhynchus weevils. The features of the mitochondrial genomes are expected to be valuable in

[1]  A. Vogler,et al.  Why barcode? High-throughput multiplex sequencing of mitochondrial genomes for molecular systematics , 2010, Nucleic acids research.

[2]  D. Wolstenholme,et al.  Animal mitochondrial DNA: structure and evolution. , 1992, International review of cytology.

[3]  Y. Ohmiya,et al.  Mitochondrial genomes of two luminous beetles, Rhagophthalmus lufengensis and R. ohbai (Arthropoda, Insecta, Coleoptera). , 2007, Gene.

[4]  D. Söll,et al.  Mitochondrial tRNA import – the challenge to understand has just begun , 2009, Biological chemistry.

[5]  Y. Han,et al.  Complete mitochondrial genome of a carabid beetle, Damaster mirabilissimus mirabilissim (Coleoptera: Carabidae) , 2012 .

[6]  A. Vogler,et al.  Mitogenome sequences stabilize the phylogenetics of weevils (Curculionoidea) and establish the monophyly of larval ectophagy. , 2013, Molecular phylogenetics and evolution.

[7]  S. Eddy,et al.  tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. , 1997, Nucleic acids research.

[8]  Julio Montoya,et al.  tRNA punctuation model of RNA processing in human mitochondria , 1981, Nature.

[9]  A. Lawrence,et al.  Evolution and Classification of Beetles , 1982 .

[10]  Michael C Whitlock,et al.  The incomplete natural history of mitochondria , 2004, Molecular ecology.

[11]  S. Cameron.  How to sequence and annotate insect mitochondrial genomes for systematic and comparative genomics research , 2014 .

[12]  S. Seo,et al.  Complete mitochondrial genome sequence of the yellow-spotted long-horned beetle Psacothea hilaris (Coleoptera: Cerambycidae) and phylogenetic analysis among coleopteran insects , 2009, Molecules and cells.

[13]  C. Simon,et al.  Incorporating molecular evolution into phylogenetic analysis, and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA , 2006 .

[14]  B. Jin,et al.  The mitochondrial genome of the firefly, Pyrocoelia rufa: complete DNA sequence, genome organization, and phylogenetic analysis with other insects. , 2004, Molecular phylogenetics and evolution.

[15]  J. Boore,et al.  Beyond linear sequence comparisons: the use of genome-level characters for phylogenetic reconstruction , 2004, Philosophical Transactions of the Royal Society B: Biological Sciences.

[16]  S. Cameron.  Insect mitochondrial genomics: implications for evolution and phylogeny. , 2014, Annual review of entomology.

[17]  J. Taanman,et al.  The mitochondrial genome: structure, transcription, translation and replication. , 1999, Biochimica et biophysica acta.

[18]  Robert S. Anderson,et al.  Weevils, weevils, weevils everywhere* , 2007 .

[19]  B. Lang,et al.  Mitochondrial genomes: anything goes. , 2003, Trends in genetics : TIG.

[20]  W. Kim,et al.  One-step PCR amplification of complete arthropod mitochondrial genomes. , 2001, Molecular phylogenetics and evolution.

[21]  G. Hewitt,et al.  Evolution and structural conservation of the control region of insect mitochondrial DNA , 1995, Journal of Molecular Evolution.

[22]  A. Vogler,et al.  Bulk De Novo Mitogenome Assembly from Pooled Total DNA Elucidates the Phylogeny of Weevils (Coleoptera: Curculionoidea) , 2014, Molecular biology and evolution.

[23]  C. Gissi,et al.  Evolution of the mitochondrial genome of Metazoa as exemplified by comparison of congeneric species , 2008, Heredity.

[24]  W. Brown,et al.  EVOLUTION OF ANIMAL MITOCHONDRIAL DNA: RELEVANCE FOR POPULATION BIOLOGY AND SYSTEMATICS , 1987 .

[25]  M. Nei,et al.  MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. , 2011, Molecular biology and evolution.

[26]  G. Hojunson.  When phylogenetic assumptions are violated : base compositional heterogeneity and among-site rate variation in beetle mitochondrial phylogenomics , 2010 .

[27]  G. Benson,et al.  Tandem repeats finder: a program to analyze DNA sequences. , 1999, Nucleic acids research.

[28]  T. Hunt,et al.  A Comprehensive Phylogeny of Beetles Reveals the Evolutionary Origins of a Superradiation , 2007, Science.

[29]  G. Hewitt,et al.  Insect mitochondrial control region: A review of its structure, evolution and usefulness in evolutionary studies , 1997 .

[30]  Xiaoji Wang,et al.  The mitochondrial genome of Dastarcus helophoroides (Coleoptera: Bothrideridae) and related phylogenetic analyses. , 2015, Gene.

[31]  P. Stadler,et al.  MITOS: improved de novo metazoan mitochondrial genome annotation. , 2013, Molecular phylogenetics and evolution.

[32]  Hao Zheng,et al.  Assessing potential biological control of the invasive plant, tree-of-heaven, Ailanthus altissima , 2006 .

[33]  J. Boore.  Animal mitochondrial genomes. , 1999, Nucleic acids research.

[34]  Hao Zheng,et al.  Biological control of invasive plants through collaboration between China and the United States of America: a perspective , 2006, Biological Invasions.

[35]  C. Cheng,et al.  Complete mitochondrial genome sequence of Cheirotonus jansoni (Coleoptera: Scarabaeidae). , 2014, Genetics and molecular research : GMR.