Conformation and cooperative order-disorder transition in aqueous solutions of β-1,3-d-glucan with different degree of branching varied by the Smith degradation.

β-1,3-d-glucan with different degrees of branching were obtained by selectively and gradually removing side chains from schizophyllan, a water-soluble triple helical polysaccharide, using the Smith degradation. Size exclusion chromatography combined with a multi-angle light scattering detection was performed in aqueous 0.1 M NaCl. The degree of branching decreased after the Smith degradation, while the molar mass distributions were almost unchanged. The molecular conformation of the Smith-degraded β-1,3-d-glucan was analyzed on the basis of the molar mass dependency of the radius gyration, and found to be comparable to the original triple helix of schizophyllan. Differential scanning calorimetry in deuterium oxide-hexadeuterodimethylsulfoxide mixtures was performed to investigate the effects of the degree of branching on the cooperative order-disorder transition. Removal of side chains affects both the transition temperature and transition enthalpy. The ordered structure is formed by the residual side chains in the triplex unit, so that the linear cooperative system of the triplex is maintained after the Smith degradation.

[1]  K. Yoshiba,et al.  Cooperative Order-Disorder Transition of Carboxylated Schizophyllan in Water-Dimethylsulfoxide Mixtures. , 2018, The journal of physical chemistry. B.

[2]  K. Yoshiba,et al.  Effects of carboxylation of the side chains on the order-disorder transition in aqueous solution of schizophyllan, a triple helical polysaccharide. , 2017, Carbohydrate polymers.

[3]  K. Yoshiba,et al.  Conformation of carboxylated schizophyllan in aqueous solution. , 2015, Carbohydrate polymers.

[4]  Michael E. Danielson,et al.  Modification of the degree of branching of a beta‐(1,3)‐glucan affects aggregation behavior and activity in an oxidative burst assay , 2015, Biopolymers.

[5]  C. Coban,et al.  Nonagonistic Dectin-1 ligand transforms CpG into a multitask nanoparticulate TLR9 agonist , 2014, Proceedings of the National Academy of Sciences.

[6]  T. Satoh,et al.  Structural Insights into Recognition of Triple-helical β-Glucans by an Insect Fungal Receptor , 2011, The Journal of Biological Chemistry.

[7]  Lina Zhang,et al.  Multiple conformation transitions of triple helical lentinan in DMSO/water by microcalorimetry. , 2009, The journal of physical chemistry. B.

[8]  C. Mazzuca,et al.  Theoretical and experimental study on a self-assembling polysaccharide forming nanochannels: static and dynamic effects induced by a soft confinement. , 2008, The journal of physical chemistry. B.

[9]  K. Uezu,et al.  Molecular dynamics studies of side chain effect on the beta-1,3-D-glucan triple helix in aqueous solution. , 2008, Biomacromolecules.

[10]  S. McIlroy,et al.  Biochemistry and molecular biology of exocellular fungal β‐(1,3)‐ and β‐(1,6)‐glucanases , 2007 .

[11]  T. Kunitake,et al.  Removal of the side‐chain glucose groups from schizophyllan improves the thermal stability of the polycytidylic acid complexes under the physiological conditions , 2004, Biopolymers.

[12]  A. Teŕamoto,et al.  Static water structure detected by heat capacity measurements on aqueous solutions of a triple-helical polysaccharide schizophyllan. , 2003, Biomacromolecules.

[13]  K. Yoshiba,et al.  Chiral Interactions in Polymer Liquid Crystals Reflecting Polymer Conformations: Triple-Helical Polysaccharide Schizophyllan and Poly(γ-benzyll-glutamate) , 2003 .

[14]  A. Teŕamoto,et al.  Ordering in aqueous polysaccharide solutions. II. Optical rotation and heat capacity of aqueous solutions of a triple-helical polysaccharide schizophyllan. , 2002, Biopolymers.

[15]  A. Teŕamoto,et al.  Ordering in aqueous polysaccharide solutions. I. Dielectric relaxation in aqueous solutions of a triple-helical polysaccharide schizophyllan. , 2002, Biopolymers.

[16]  B. Christensen,et al.  Gelation of periodate oxidised scleroglucan (scleraldehyde) , 2001 .

[17]  A. Teŕamoto.  Cooperative conformational transitions in linear macromolecules undergoing chiral perturbations , 2001 .

[18]  S. Shinkai,et al.  Molecular Recognition of Adenine, Cytosine, and Uracil in a Single-Stranded RNA by a Natural Polysaccharide: Schizophyllan , 2000 .

[19]  A. Teŕamoto,et al.  Dielectric study of the cooperative order–disorder transition in aqueous solutions of schizophyllan, a triple‐helical polysaccharide , 1995, Biopolymers.

[20]  A. Elgsaeter,et al.  An antitumor, branched (1-->3)-beta-D-glucan from a water extract of fruiting bodies of Cryptoporus volvatus. , 1994, Carbohydrate research.

[21]  T. Matsuo,et al.  Solvent effects on the cooperative order–disorder transition of aqueous solutions of schizophyllan, a triple‐helical polysaccharide , 1990, Biopolymers.

[22]  C. Hara,et al.  A differential scanning calorimetric study of the conformat ional transitions of several kinds of (1→6) branched (1→3)-β-d-glucans in a mixture of water and dimethylsulfoxide , 1990 .

[23]  S. Kitamura,et al.  A differential scanning calorimetric study of the conformational transitions of schizophyllan in mixtures of water and dimethylsulfoxide , 1989, Biopolymers.

[24]  A. Teŕamoto,et al.  Isotope effect on the order-disorder transition in aqueous schizophyllan , 1987 .

[25]  S. Takeuchi,et al.  Clinical evaluation of schizophyllan combined with irradiation in patients with cervical cancer: A randomized controlled study , 1986, Cancer.

[26]  A. Teŕamoto,et al.  Ordered structure in aqueous polysaccharide. 5. Cooperative order-disorder transition in aqueous schizophyllan , 1986 .

[27]  T. Norisuye.  Triple‐stranded helical structure of schizophyllan and its antitumor activity in aqueous solution , 1985 .

[28]  A. Teŕamoto,et al.  A Thermal Transition in a Cholesteric Liquid Crystal of Aqueous Schizophyllan , 1984 .

[29]  A. Teŕamoto,et al.  Triphase equilibrium in aqueous solutions of the rodlike polysaccharide schizophyllan , 1984 .

[30]  H. Fujita,et al.  Triple helix of Schizophyllum commune polysaccharide in dilute solution. 4. Light scattering and viscosity in dilute aqueous sodium hydroxide , 1981 .

[31]  H. Fujita,et al.  Triple helix of aschizophyllum commune polysaccharide in aqueous solution , 1980 .

[32]  K. Kimura,et al.  Polysaccharide Produced by Schizophyllum commune Part II , 1970 .