A review. Possible sources of ethanol ante- and post-mortem: its relationship to the biochemistry and microbiology of decomposition.

1 . Introduction . . . . . . . . . . 2 . Methods of ethanol determination . . . . . . A . Chemical methods . . . . . . . . B . Enzymic method . . . . . . . . C . Gas chromatographic methods 3 . Levels of ‘Endogenous’ ethanol . . . . . . . 4 . Evidence of post-mortem production of ethanol . . . . A . Ethanolformationindead animals . . . . . B . Ethanol formationin hurnancorpses C . Ethanol formationinisolated tissues . . . . . D . Conclusions . . . . . . . . . 5 . Substrates available for ethanol production A . Biochemical changes occurring in muscle prior to rigor mortis B . Glucose and glycogen levels in tissues . . . . . C . Other substrates . . . . . . . . . (i) Ribose . . . . . . . . . (ii) Amino acids . . . . . . . . (iii) Lactate . . . . . . . . . D . Processes during putrefaction (glycerol production) . . E . Conclusions . . . . . . . . . 6 . ‘Post-mortem’ microbiology . . . . . . . A . Numbers . . . . . . . . . . B . Effect of mode of death . . . . . . . C . Microflora of lymph nodes . . . . . . . D . ‘Bone taint’ . . . . . . . . . E . Types of organisms isolated from blood and other tissues . F . Organisms that cause decomposition (putrefaction) . . C . Mode of spread of bacteria in human corpses H . Conclusions . . . . . . . . .

[1]  B. Hood,et al.  Nitrogen, lipid, glycogen and deoxyribonucleic acid content of human liver. The effect of brief starvation and intravenous administration of glucose. , 2009, Acta medica Scandinavica.

[2]  H. M. Stevens,et al.  Identification tests for bases formed during the putrefaction of visceral material. , 2009, Acta pharmacologica et toxicologica.

[3]  H. Theorell,et al.  Development of ethanol in blood samples and human organs during forensic chemical practice. , 2009, Acta pharmacologica et toxicologica.

[4]  W. Bonte,et al.  Postmortem dating of putrefied material through ptomaine estimation. , 1977, Journal of forensic sciences.

[5]  J. Simonsen,et al.  Early Formation of Adipocere in Temperate Climate , 1977, Medicine, science, and the law.

[6]  K. Tomita On putrefactions and floatations of dead bodies under water (supplement). , 1976, Hiroshima journal of medical sciences.

[7]  N. Waterman,et al.  Demonstration in vitro of postmortem bacterial transmigration. , 1976, American journal of clinical pathology.

[8]  R. Pankhurst,et al.  A new key to the yeasts. , 1976 .

[9]  C. Gill,et al.  Effect of delayed evisceration on the microbial quality of meat , 1976, Applied and environmental microbiology.

[10]  G. Lees,et al.  Formation of acetaldehyde from threonine by lactic acid bacteria , 1976, Journal of Dairy Research.

[11]  U. Haglund,et al.  Mucosal lesions in the human small intestine in shock. , 1975, Gut.

[12]  P. Holt,et al.  Endogenous ethanol production and hepatic disease following jejunoileal bypass for morbid obesity. , 1975, The American journal of clinical nutrition.

[13]  K. Tomita On putrefactions and floatations of dead bodies under water. , 1975, Hiroshima journal of medical sciences.

[14]  M. D. Smith,et al.  Differential amperometric determination of alcohol in blood or urine using alcohol dehydrogenase. , 1975, Analytical chemistry.

[15]  R. E. Buchanan,et al.  Bergey's Manual of Determinative Bacteriology. , 1975 .

[16]  W. B. Stavinoha,et al.  THE LEVELS OF LABILE INTERMEDIARY METABOLITES IN MOUSE BRAIN FOLLOWING RAPID TISSUE FIXATION WITH MICROWAVE IRRADIATION 1 , 1975, Journal of neurochemistry.

[17]  H. O. Bagadi,et al.  A study of the route of dissemination of orally administered spores of Clostridium novyi type B in guinea-pigs and sheep. , 1974, Research in veterinary science.

[18]  W. Franks,et al.  Postmortem myocardial tissue lactate and potassium levels in fatal aircraft accidents. , 1974, Aerospace medicine.

[19]  Watson Wj,et al.  Evaluation of tissue postmortem lactates in accident investigation using an animal model. , 1974 .

[20]  R. W. White In vivo and in vitro ethanolic fermentation of milk replacers by the naturally occurring yeast Torulopsis glabrata , 1974, The Journal of Agricultural Science.

[21]  J. Gearien,et al.  Determination of ethanol in fresh and putrefied post mortem tissues. , 1973, Journal of chromatography.

[22]  L. Nilsson Liver glycogen content in man in the postabsorptive state. , 1973, Scandinavian journal of clinical and laboratory investigation.

[23]  W. Moore,et al.  Enterotoxigenic intestinal bacteria in tropical sprue. , 1973, Annals of internal medicine.

[24]  D. Lakatua,et al.  The effect of microbial contamination of the blood sample on the determination of ethanol levels in serum. , 1973, American journal of clinical pathology.

[25]  K. W. Smalldon,et al.  The stability of ethanol in stored blood: Part II. The mechanism of ethanol oxidation , 1973 .

[26]  G. Mead,et al.  Microbial changes leading to the spoilage of hung pheasants, with special reference to the clostridia. , 1973, The Journal of applied bacteriology.

[27]  R. W. White,et al.  Ethanol production from glucose by Torulopsis glabrata occurring naturally in the stomachs of newborn animals. , 1972, The Journal of applied bacteriology.

[28]  R. L. Leffert,et al.  Putrefactive ethanol sources in postmortem tissues of conventional and germ-free mice. , 1972, Archives of pathology.

[29]  Maria Gumińska,et al.  Studies on the formation of ethanol and of pyruvate as its precursor from some di- and tricarbonic compounds in putrefying blood in vitro. , 1972, Forensic science.

[30]  N. Jain,et al.  Analysis of alcohol. I. A review of chemical and infrared methods. , 1972, Journal of chromatographic science.

[31]  E. Dawes,et al.  Pyruvic acid metabolism and ethanol formation in Erwinia amylovora. , 1971, Journal of general microbiology.

[32]  G. Niwayama Postmortem blood microbiology using sterile autopsy technique. , 1971, The Tohoku journal of experimental medicine.

[33]  R. E. Ritts,et al.  Microbiological examination of postmortem tissues. , 1971, Archives of pathology.

[34]  L. Carlson,et al.  Concentration of Triglycerides, Phospholipids and Glycogen in Skeletal Muscle and of Free Fatty Acids and β‐Hydroxybutyric Acid in Blood in Man in Response to Exercise , 1971, European journal of clinical investigation.

[35]  J. Crowther Sarcina ventriculi in human faeces. , 1971, Journal of medical microbiology.

[36]  S. Kotoku,et al.  Medico-legal evaluation of the ethanol levels in cadaveric blood and urine. , 1971, Yonago acta medica.

[37]  Rösemann Gw,et al.  The microbiologic evaluation and enumeration of postmortem specimens from human remains. , 1971 .

[38]  R H Dainty,et al.  Symposium on microbial changes in foods. Changes caused by microbes in spoilage of meats. , 1971, The Journal of applied bacteriology.

[39]  T. Perry,et al.  REGIONAL DISTRIBUTION OF AMINO ACIDS IN HUMAN BRAIN OBTAINED AT AUTOPSY , 1971, Journal of neurochemistry.

[40]  D. Savage,et al.  Associations of indigenous microorganisms with gastrointestinal mucosal epithelia. , 1970, The American journal of clinical nutrition.

[41]  H. Krebs,et al.  The physiological role of liver alcohol dehydrogenase. , 1970, The Biochemical journal.

[42]  P. G. Canonico,et al.  LYSOSOMES IN SKELETAL MUSCLE TISSUE , 1970, The Journal of cell biology.

[43]  R. Dainty Purification and properties of threonine aldolase from Clostridium pasterianum , 1970 .

[44]  E. Canale-Parola,et al.  Biology of the sugar-fermenting Sarcinae. , 1970, Bacteriological reviews.

[45]  C. Vanderzant,et al.  A MICROBIOLOGICAL EXAMINATION OF MUSCLE TISSUE OF BEEF, PORK, AND LAMB CARCASSES , 1969 .

[46]  W. Krause,et al.  Fungaemia and funguria after oral administration of Candida albicans. , 1969, Lancet.

[47]  E. Barnes,et al.  Anaerobic gram negative nonsporing bacteria from the caeca of poultry. , 1968, The Journal of applied bacteriology.

[48]  D. J. Blackmore The bacterial production of ethyl alcohol. , 1968, Journal - Forensic Science Society.

[49]  E. Barnes The use of antibiotics for the preservation of poultry and meat. , 1968, Bibliotheca nutritio et dieta.

[50]  Plueckhahn Vd,et al.  Factors influencing thhe significance of alcohol concentrations in autopsy blood samples. , 1968 .

[51]  J. Shively,et al.  Postmortem bacteriology: a practical method for routine use. , 1968, American journal of clinical pathology.

[52]  Plueckhahn Vd The significance of blood alcohol levels at autopsy. , 1967 .

[53]  V. D. Plueckhahn THE SIGNIFICANCE OF BLOOD ALCOHOL LEVELS AT AUTOPSY , 1967, The Medical journal of Australia.

[54]  H L Gibbons,et al.  Alcohol-induced hypoglycemia as a factor in aircraft accidents. , 1966, Aerospace medicine.

[55]  J. von Wartburg,et al.  Alcohol Dehydrogenase in Ethanol Metabolism , 1966 .

[56]  A. Wort,et al.  Post-mortem Bacteriology , 1966 .

[57]  I. McManus,et al.  Studies on the identification and origin of ethanol in mammalian tissues. , 1966, The Journal of biological chemistry.

[58]  H. Saxena,et al.  Studies of postmortem microbiology using sterile autopsy technique. , 1965, Archives of pathology.

[59]  J. Fekete,et al.  POSTMORTEM BLOOD SUGAR AND BLOOD UREA NITROGEN DETERMINATIONS. , 1965, Canadian Medical Association journal.

[60]  R. B. Schultz,et al.  A RE-EVALUATION OF BLOOD CULTURE AS AN AUTOPSY PROCEDURE. , 1965, American journal of clinical pathology.

[61]  E. Dawes A SYMPOSIUM ON SOME COMPARATIVE ASPECTS OF INTERMEDIARY METABOLISM IN MICRO ORGANISMS COMPARATIVE ASPECTS OF ALCOHOL FORMATION , 1963 .

[62]  J. G. Sharp Aseptic autolysis in rabbit and bovine muscle during storage at 37 , 1963 .

[63]  D. Lester,et al.  The concentration of apparent endogenous ethanol. , 1962, Quarterly journal of studies on alcohol.

[64]  B. Hobbs,et al.  STUDIES ON THE GROWTH, SPORULATION AND CARRIAGE OF CLOSTRIDIUM WELCHII WITH SPECIAL REFERENCE TO FOOD POISONING STRAINS , 1961 .

[65]  D. Lester Endogenous ethanol: a review. , 1961 .

[66]  G. Bourne,et al.  STRUCTURE AND FUNCTION OF MUSCLE , 1961 .

[67]  J. Kevorkian,et al.  Transfusion of postmortem human blood. , 1961, American journal of clinical pathology.

[68]  I. Bersohn,et al.  The influence of diet on serumtriglycerides in South Africa white and Bantu prisoners. , 1961, Lancet.

[69]  M. Yueh,et al.  Beef Aroma, Some Volatile Constituents of Cooked Beef , 1960 .

[70]  P. M. Nottingham Bone-taint in beef. II. Bacteria in ischiatic lymph nodes. , 1960 .

[71]  M. M. Tarasov CADAVERIC BLOOD TRANSFUSION , 1960, Annals of the New York Academy of Sciences.

[72]  C. de Duve,et al.  Tissue fractionation studies. 10. Influence of ischaemia on the state of some bound enzymes in rat liver. , 1959, The Biochemical journal.

[73]  D. M. Doty,et al.  Enzymes in Beef Muscle, Over-All Assay and Partial Purification Procedures for Proteolytic Enzymes in Beef Muscle , 1959 .

[74]  M. Starr,et al.  ANAEROBIC DISSIMILATION OF GLUCOSE BY ERWINIA AMYLOVORA , 1959, Journal of bacteriology.

[75]  J. J. Kurtin Studies in autopsy bacteriology. , 1958, American journal of clinical pathology.

[76]  B. Fell,et al.  Changes in the Intestinal Mucosa of the Sheep following Death by Humane Killer , 1957, Nature.

[77]  H. Gifford,et al.  Erroneous blood alcohol findings at autopsy; avoidance by proper sampling technique. , 1957, Journal of the American Medical Association.

[78]  N. Kaplan,et al.  Yeast Alcohol Dehydrogenase. III. Relation of Alcohol Structure to Activity1 , 1957 .

[79]  E. Dawes,et al.  The formation of ethanol in Escherichia coli. , 1956, Biochimica et biophysica acta.

[80]  B. Marsh,et al.  Bone‐taint in beef , 1956 .

[81]  M. Ingram,et al.  THE EFFECT OF REDOX POTENTIAL ON THE GROWTH OF CLOSTRIDIUM WELCHII STRAINS ISOLATED FROM HORSE MUSCLE , 1956 .

[82]  Pritchard Je,et al.  The increase in serious staphylococcal infections as shown by post-mortem investigation. , 1955 .

[83]  M. Ingram,et al.  Changes in the oxidation—reduction potential of the Sterno‐cephalicus muscle of the horse after death in relation to the development of bacteria , 1955 .

[84]  H. Tallan,et al.  Studies on the free amino acids and related compounds in the tissues of the cat. , 1954, The Journal of biological chemistry.

[85]  B. Magasanik,et al.  METABOLIC PATHWAYS OF GLYCEROL DISSIMILATION A , 1953, Journal of bacteriology.

[86]  F. Schweinburg,et al.  Bacteriology of the Healthy Experimental Animal , 1953 .

[87]  H. Tarr Ribose and the Maillard Reaction in Fish Muscle , 1953, Nature.

[88]  H. H. Weiser,et al.  A Microbiological Study of Lymph Nodes, Bone Marrow and Muscle Tissue Obtained from Slaughtered Cattle , 1953 .

[89]  M. Ingram Internal bacterial taints (‘bone taint’ or ‘souring’) of cured pork legs , 1952, Journal of Hygiene.

[90]  R. Fisher,et al.  Results of the Studies on the Determination of Ethyl Alcohol in Tissues , 1951 .

[91]  H. Tarr The acid-soluble phosphorus com pounds of fish skeletal muscle. , 1950 .

[92]  J. Tonge,et al.  THE POST‐MORTEM BLOOD SUGAR , 1949, The Medical journal of Australia.

[93]  J. Hildes,et al.  Liver and muscle glycogen in normal subjects, in diabetes mellitus and in acute hepatitis; under basal conditions. , 1949, Clinical science.

[94]  J. Tepperman,et al.  The in vivo inactivation by cyanide of brain cytochrome oxidase and its effect on glycolysis and on the high energy phosphorus compounds in brain. , 1946, The Journal of biological chemistry.

[95]  D. L. Cramer,et al.  The component fatty acids of human depot fat. , 1943 .

[96]  R. McLean,et al.  Biochemical Changes in Body Fluids After Death , 1943 .

[97]  I. C. Gunsalus,et al.  THE EFFECT OF pH ON THE LACTIC ACID FERMENTATION , 1942 .

[98]  C. Hine,et al.  Method for Determination of Ethyl Alcohol for Medicolegal Purposes , 1941 .

[99]  L. B. Jensen,et al.  A STUDY OF HAN SOURING , 1941 .

[100]  M. Mickelson,et al.  The Dissimilation of Glycerol by Coli-Aerogenes Intermediates , 1940, Journal of bacteriology.

[101]  Elsie Johnson,et al.  Post‐mortem glycolysis , 1940 .

[102]  S. S. Yudin TRANSFUSION OF STORED CADAVER BLOOD: PRACTICAL CONSIDERATIONS : THE FIRST THOUSAND CASES , 1937 .

[103]  W. N. Shamov THE TRANSFUSION OF STORED CADAVER BLOOD , 1937 .

[104]  S. E. Kerr The carbohydrate metabolism of brain. 1. The determination of glycogen in nerve tissue. , 1936 .

[105]  S. S. Yudin TRANSFUSION OF CADAVER BLOOD , 1936 .

[106]  C. Burn Experimental Studies of Postmortem Bacterial Invasion in Animals , 1934 .

[107]  F. Tanner,et al.  Penetration of Intestinal Wall of Rabbit by Yeasts , 1932 .

[108]  E. Epstein,et al.  The Significance of Postmortem Bacteriological ExaminationWith Special Reference to Streptococci and Enterococci , 1929 .

[109]  A. Reith BACTERIA IN THE MUSCULAR TISSUES AND BLOOD OF APPARENTLY NORMAL ANIMALS , 1926, Journal of bacteriology.

[110]  F. Humphreys Formation of acrolein from glycerol by B. welchii , 1924 .

[111]  A. Giordano Studies in Postmortem Bacteriology , 1922 .

[112]  E. Widmark Eine Modifikation der Niclouxschen Methode zur Bestimmung von Äthylalkohol , 1920 .

[113]  D. Richey,et al.  Studies on Bacteriemias in the Agonal Period. , 1918, The Journal of medical research.

[114]  R. F. Ruttan,et al.  THE COMPOSITION OF ADIPOCERE , 1917 .

[115]  R. Barnes ON A CASE OF SARCINA VENTRICULI ASSOCIATED WITH FATTY DEGENERATION OF THE LIVER. , 1854 .

[116]  J. Oliver,et al.  The detection, identification and measurement of indole, tryptamine and 2-phenethylamine in putrefying human tissue. , 1977, Forensic science.

[117]  P. Gouet,et al.  Blood poisonings at slaughter and their consequences. , 1977, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe B: Hygiene, praventive Medizin.

[118]  Gilbert Ef,et al.  Glycogen complexes in muscle in Reye's syndrome simulating virus-like particles. , 1977 .

[119]  E. Gilbert,et al.  Glycogen complexes in muscle in Reye's syndrome simulating virus-like particles. , 1977, Laboratory investigation; a journal of technical methods and pathology.

[120]  W. Bonte,et al.  Experimental investigations in post-mortem protein degradation. , 1976, Forensic science.

[121]  R. Hurley,et al.  Microflora of the vagina during pregnancy. , 1974, Society for Applied Bacteriology symposium series.

[122]  E. Koneman,et al.  Postmortem bacteriology. 3. Clinical significance of microorganisms recovered at autopsy. , 1974, American journal of clinical pathology.

[123]  F. A. Skinner,et al.  The normal microbial flora of man. , 1974 .

[124]  B. S. Drasar,et al.  Human intestinal flora. , 1974 .

[125]  W. J. Watson,et al.  Evaluation of tissue postmortem lactates in accident investigation using an animal model. , 1974, Aerospace medicine.

[126]  K. W. Smalldon,et al.  The stability of ethanol in stored blood. II. The mechanism of ethanol oxidation. , 1973, Analytica chimica acta.

[127]  R. Shank,et al.  Post mortem changes in the content and specific radioactivity of several amino acids in four areas of the rat brain. , 1971, Journal of neurobiology.

[128]  G. Alderson,et al.  Studies in postmortem bacteriology. I. Necropsy sterility in three patients as long as thirty-five days postmortem. , 1971, American journal of clinical pathology.

[129]  E. Koneman,et al.  Postmortem bacteriology. II. Selection of cases for culture. , 1971, American journal of clinical pathology.

[130]  T. Bauchop Stomach microbiology of primates. , 1971, Annual review of microbiology.

[131]  T. Gavan,et al.  Anaerobic bacteria in postmortem blood cultures. Correlation with lesions of the gastrointestinal tract. , 1971, Cleveland Clinic quarterly.

[132]  R. Hockett,et al.  The microbiologic evaluation and enumeration of postmortem specimens from human remains. , 1971, Health laboratory science.

[133]  M. Gumińska,et al.  Studies on the formation of endogenous ethanol in blood putrefying in vitro. , 1970, Journal of forensic medicine.

[134]  E. Koneman,et al.  Postmortem bacteriology. , 1970, CRC critical reviews in clinical laboratory sciences.

[135]  B. Kaempe Interfering compounds and artifacts in the identification of drugs in autopsy material. , 1969, Progress in chemical toxicology.

[136]  E. Hultman Muscle glycogen in man determined in needle biopsy specimens: method and normal values. , 1967, Scandinavian journal of clinical and laboratory investigation.

[137]  R. Dainty Purification and properties of threonine aldolase from Clostridium pasteurianum. , 1967, The Biochemical journal.

[138]  P. Levine,et al.  Microbiology experience in collection of human tissue. , 1966, American journal of clinical pathology.

[139]  Narayan Kg Studies on Clostridia incidence in the beef cattle. , 1966 .

[140]  Narayan Kg,et al.  Incidence of Clostridia in emergency-slaughtered cattle. , 1966, Acta veterinaria Academiae Scientiarum Hungaricae.

[141]  G. W. Walker,et al.  Determination of ethanol in blood by gas chromatography. , 1966, The Analyst.

[142]  K. G. Narayan Studies on Clostridia incidence in the beef cattle. , 1966, Acta veterinaria Academiae Scientiarum Hungaricae.

[143]  O. H. Lowry,et al.  EFFECT OF ISCHEMIA ON KNOWN SUBSTRATES AND COFACTORS OF THE GLYCOLYTIC PATHWAY IN BRAIN. , 1964, The Journal of biological chemistry.

[144]  Wilkins Rm,et al.  AUTOPSY BACTERIOLOGY: REVIEW OF 2,033 CASES. , 1964 .

[145]  R. Wilkins,et al.  AUTOPSY BACTERIOLOGY: REVIEW OF 2,033 CASES. , 1964, Archives of pathology.

[146]  E. Dawes COMPARATIVE ASPECTS OF ALCOHOL FORMATION. , 1963, Journal of general microbiology.

[147]  R. Furbank,et al.  Adipocere - a Review , 1957 .

[148]  R. Lawrie Residual glycogen at high ultimate pH in horse muscle. , 1955, Biochimica et biophysica acta.

[149]  W. Schwerd [Evaluation of alcohol findings in cadaver blood]. , 1954, Deutsche Zeitschrift fur die gesamte gerichtliche Medizin.

[150]  M. Ingram,et al.  Whalemeat: Bacteriology and Hygiene. , 1953 .

[151]  C. Lenti,et al.  Über die Spaltung von Threonin durch Escherichia coli , 1953 .

[152]  C. Garhammer,et al.  [On the detection of lower primary alcohols of the aliphatic group and their formation in cadaveric blood]. , 1953, Deutsche Zeitschrift fur die gesamte gerichtliche Medizin.

[153]  H. H. Weiser,et al.  A microbiological study of lymph nodes, bone marrow and muscle tissue obtained from slaughtered cattle. , 1953, Applied microbiology.

[154]  H. Redetzki,et al.  [Putrefaction and ethyl alcohol]. , 1952, Deutsche Zeitschrift fur die gesamte gerichtliche Medizin.

[155]  H. Theorell,et al.  An enzymatic method for the microdetermination of ethanol. , 1951, Scandinavian journal of clinical and laboratory investigation.

[156]  C. Adamson A Bacteriological Study of Lymph Nodes. Analysis of Postmortem Specimens with particular reference to Clinical, Serologieal and Histo-Pathologieal Findings. , 1949 .

[157]  S. T. Cowan Bergey's Manual of Determinative Bacteriology , 1948, Nature.

[158]  C. T. Calam,et al.  Studies on the biochemistry of micro-organisms: 74. The molecular constitution of geodin and erdin, two chlorine-containing metabolic products of Aspergillus terreus Thom. Part 3. Possible structural formulae for geodin and erdin. , 1947, The Biochemical journal.

[159]  R. Stanier,et al.  The nature of the Aeromonas fermentation. , 1944, The Biochemical journal.

[160]  J. Smit The biology of the fermenting sarcinæ , 1933 .

[161]  E. Widmark Eine Modifikation der Niclouxschen Methode zur Bestimmung von Äthylalkohol1 , 1918 .

[162]  E. Widmark Über die Konzentration des genossenen Alkohols in Blut und Harn unter verschiedenen Umständen1 , 1916 .

[163]  E. Southard,et al.  The significance of Bacteria cultivated from the Human Cadaver: a Second Series of One Hundred Cases of Mental Disease, with Blood and Cerebrospinal Fluid Cultures and Clinical and Histological Correlations. , 1915, The Journal of medical research.

[164]  A. Harden LXIV.—The chemical action of Bacillus coli communis and similar organisms on carbohydrates and allied compounds , 1901 .