Effect of fermented rice culture on the microbiological, biochemical and sensory characteristics of low-salt douchi, a traditional Chinese fermented soybean condiment

Summary A low-salt (4% NaCl, w/w) douchi supplemented with different concentration of fermented rice culture (FRC) of 0%, 5%, 10%, 15% and 20% (v/w) was produced, and the effect of FRC on microbiology, biochemistry and sensory quantity was investigated. Results indicated that FRC had a significant effect on low-salt douchi. Compared with the control (added 0% FRC), in the other treatments, the counts of yeast and lactic acid bacteria increased by about 50%, whereas the moulds decreased by about 30%; the pH reduction was close to 4.7; total acid increased to about 1.300 g per 100 g; reducing sugar maintained a decrease to 21.07 mg per g; amino nitrogen increased to about 0.900 g per 100 g, but not above the control (1.013 g per 100 g). In general terms, total free amino acid contents and three organic acids were higher in low-salt products than four commercial products. Sensory evaluation of products supplemented with 10% and 15% FRC revealed better overall acceptability compared with other treatments and a commercial product.

[1]  Xin Lu,et al.  Changes in Angiotensin I-Converting Enzyme Inhibitory Activities During the Ripening of Douchi (a Chinese Traditional Soybean Product) Fermented by Various Starter Cultures , 2010 .

[2]  B. Bhandari,et al.  Changes During Processing and Sodium Chloride Supplementation on the Physical and Chemical Properties of Douchi , 2010 .

[3]  Qiao Fadong.  Research progress on rice wine starters in the world , 2010 .

[4]  Wei Chen,et al.  Changes in microbial community during Chinese traditional soybean paste fermentation , 2009 .

[5]  Peng Liu,et al.  Microbiological and biochemical changes during processing of the traditional Chinese food douzhi , 2009 .

[6]  R. Chiou.  Proteolysis During the Fermentation of Ethanol‐Supplemented Miso , 2008 .

[7]  Xiao Dong Chen,et al.  In vitro and in vivo studies on the antioxidant activities of the aqueous extracts of Douchi (a traditional Chinese salt-fermented soybean food) , 2008 .

[8]  Y. Tsai,et al.  Diversity of lactic acid bacteria in fermented brines used to make stinky tofu. , 2008, International journal of food microbiology.

[9]  X. Ding,et al.  Evolution of Proteolytic Tasty Components During Preparation of Douchiba, a Traditional Chinese Soy-Fermented Appetizer , 2007 .

[10]  Li-te Li,et al.  Chemical components of Aspergillus‐type Douchi, a Chinese traditional fermented soybean product, change during the fermentation process , 2007 .

[11]  Dong Li,et al.  Influences of processing and NaCl supplementation on isoflavone contents and composition during douchi manufacturing , 2007 .

[12]  Yongqiang Cheng,et al.  Anti-α-glucosidase activity of Chinese traditionally fermented soybean (douchi) , 2007 .

[13]  Y-s Chen,et al.  Isolation and characterization of lactic acid bacteria from dochi (fermented black beans), a traditional fermented food in Taiwan , 2006, Letters in applied microbiology.

[14]  Li-te Li,et al.  Angiotensin I-converting enzyme inhibitory peptides in douchi, a Chinese traditional fermented soybean product , 2006 .

[15]  B. Han,et al.  Microbial changes during the production of Sufu––a Chinese fermented soybean food , 2004 .

[16]  Seung-Ho Kim,et al.  Evaluation of taste compounds in water-soluble extract of a doenjang (soybean paste) , 2003 .

[17]  Yi-Zheng Zhang,et al.  Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. , 2003, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[18]  R. Chiou.  Salt-free Miso Fermentation Using Ethanol, Sugars, and Polyols , 1999 .

[19]  H. Chung.  Volatile components in fermented soybean (Glycine max) curds. , 1999, Journal of agricultural and food chemistry.

[20]  L. Beuchat,et al.  Fermentation of low-salt miso as affected by supplementation with ethanol. , 1999, International journal of food microbiology.

[21]  M. Oruña‐Concha,et al.  Determination of Amino Acids in Green Beans by Derivatization with Phenylisothiocianate and High-Performance Liquid Chromatography with Ultraviolet Detection , 1997 .

[22]  G. Nychas,et al.  Microbiological, physicochemical and organoleptic changes of shredded carrots stored under modified storage , 1996 .

[23]  V. Abram,et al.  Isolation of low-molecular-mass hydrophobic bitter peptides in soybean protein hydrolysates by reversed-phase high-performance liquid chromatography☆ , 1995 .

[24]  A. H. Stouthamer,et al.  Physical Factors Influencing Microbial Interactions and Biochemical Changes during the Baceman Stage of Indonesian Kecap (Soy Sauce) Production , 1994 .

[25]  Judoamidjojo Muljono.  The studies on kecap-indigenous seasoning of Indonesia , 1985 .

[26]  B. Wood,et al.  Microbiology and Biochemistry of Miso (Soy Paste) Fermentation , 1982 .

[27]  Akimitsu Kimizuka,et al.  Contribution of peptides and amino acids to the taste of foods , 1969 .

[28]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .