Photoelectrochemical lab-on-paper device based on molecularly imprinted polymer and porous Au-paper electrode.

In this work, microfluidic paper-based analytical device (μ-PAD) was applied in a photoelectrochemical (PEC) method and thus a truly low-cost, simple, portable, and disposable microfluidic PEC origami device (μ-PECOD) was demonstrated. The molecular imprinting technique was introduced into microfluidic paper-based analytical devices (μ-PADs) through electropolymerization of molecular imprinted polyaniline (MPANI) in a novel Au nanoparticle (AuNP)-modified paper working electrode (Au-PWE). This is fabricated through the growth of an AuNP layer on the surfaces of cellulose fibers in the PWE. Under visible light irradiation, MPANI can generate the photoelectric transition from the highest occupied molecular orbital (HOMO) to the lowest unoccupied molecular orbital (LUMO), delivering the excited electrons to the AuNPs, and then to the carbon working electrode. Simultaneously, it is believed that a positively charged hole of MPANI that took part in the oxidation process was consumed by ascorbic acid (AA) to promote the amplifying photocurrent response. On the basis of this novel MPANI-Au-PWE and the principle of origami, a microfluidic molecular imprinted polymer (MIP)-based photoelectrochemical analytical origami device (μ-MPECOD), comprised of an auxiliary tab and a sample tab, is developed for the detection of heptachlor in the linear range from 0.03 nmol L(-1) to 10.0 nmol L(-1) with a low detection limit of 8.0 pmol L(-1). The selectivity, reproducibility, and stability of this μ-MPECOD are investigated. This μ-MPECOD would provide a new platform for high-throughput, sensitive, specific, and multiplex assay in public health, environmental monitoring, and the developing world.

[1]  Robert Pelton,et al.  Bioactive paper provides a low-cost platform for diagnostics , 2009, TrAC Trends in Analytical Chemistry.

[2]  Zhihong Nie,et al.  Programmable diagnostic devices made from paper and tape. , 2010, Lab on a chip.

[3]  Monica Lira-Cantu,et al.  Vertically-aligned nanostructures of ZnO for excitonic solar cells: a review , 2009 .

[4]  Itamar Willner,et al.  Electrochemical, photoelectrochemical, and piezoelectric analysis of tyrosinase activity by functionalized nanoparticles. , 2008, Analytical chemistry.

[5]  George M Whitesides,et al.  Integration of paper-based microfluidic devices with commercial electrochemical readers. , 2010, Lab on a chip.

[6]  Wantai Yang,et al.  A study on the synthesis, characterization and properties of polyaniline using acrylic acid as a primary dopant. I: polymerization and polymer , 2005 .

[7]  Donal D. C. Bradley,et al.  The Effect of Polymer Optoelectronic Properties on the Performance of Multilayer Hybrid Polymer/TiO2 Solar Cells , 2005 .

[8]  Shenguang Ge,et al.  Paper-based electrochemiluminescent 3D immunodevice for lab-on-paper, specific, and sensitive point-of-care testing. , 2012, Chemistry.

[9]  Rongning Liang,et al.  Potentiometric sensing of neutral species based on a uniform-sized molecularly imprinted polymer as a receptor. , 2010, Angewandte Chemie.

[10]  Guang-Li Wang,et al.  Selective detection of trace amount of Cu2+ using semiconductor nanoparticles in photoelectrochemical analysis. , 2010, Nanoscale.

[11]  Emanuel Carrilho,et al.  Paper microzone plates. , 2009, Analytical chemistry.

[12]  I. Willner,et al.  Semiconductor quantum dots for bioanalysis. , 2008, Angewandte Chemie.

[13]  Shenguang Ge,et al.  Electrochemical DNA sensor based on three-dimensional folding paper device for specific and sensitive point-of-care testing , 2012 .

[14]  Jinghua Yu,et al.  Visible light photoelectrochemical sensor based on Au nanoparticles and molecularly imprinted poly(o-phenylenediamine)-modified TiO2 nanotubes for specific and sensitive detection chlorpyrifos. , 2013, The Analyst.

[15]  Jinghua Yu,et al.  Microfluidic paper-based chemiluminescence biosensor for simultaneous determination of glucose and uric acid. , 2011, Lab on a chip.

[16]  Jinghua Yu,et al.  Photoelectrochemical Sensor Based on Molecularly Imprinted Polymer-Coated TiO2 Nanotubes for Lindane Specific Recognition and Detection , 2013, Journal of Inorganic and Organometallic Polymers and Materials.

[17]  G. Whitesides,et al.  Patterned paper as a platform for inexpensive, low-volume, portable bioassays. , 2007, Angewandte Chemie.

[18]  Roberto Argazzi,et al.  Photoelectrochemical behavior of sensitized TiO(2) Photoanodes in an aqueous environment: application to hydrogen production. , 2010, Inorganic chemistry.

[19]  T. Tatsuma,et al.  Photoelectrochemical and Optical Behavior of Single Upright Ag Nanoplates on a TiO2 Film , 2011 .

[20]  C. Cai,et al.  Polyaniline Nanofibers: Synthesis, Characterization, and Application to Direct Electron Transfer of Glucose Oxidase , 2009 .

[21]  George M. Whitesides,et al.  Patterning precipitates of reactions in paper , 2010 .

[22]  Mohammad Faghri,et al.  A fluidic diode, valves, and a sequential-loading circuit fabricated on layered paper. , 2012, Lab on a chip.

[23]  Orawon Chailapakul,et al.  Electrochemical detection for paper-based microfluidics. , 2009, Analytical chemistry.

[24]  G. Whitesides,et al.  Three-dimensional microfluidic devices fabricated in layered paper and tape , 2008, Proceedings of the National Academy of Sciences.

[25]  T. Fabritius,et al.  Refractive index matching improves optical object detection in paper , 2008 .

[26]  L. M. Davies,et al.  Development of a bioactive paper sensor for detection of neurotoxins using piezoelectric inkjet printing of sol-gel-derived bioinks. , 2009, Analytical chemistry.

[27]  Xiaoru Zhang,et al.  A new photoelectrochemical aptasensor for the detection of thrombin based on functionalized graphene and CdSe nanoparticles multilayers. , 2011, Chemical communications.

[28]  Zhixiang Wei,et al.  Hollow Microspheres of Polyaniline Synthesized with an Aniline Emulsion Template , 2002 .

[29]  K. Richards,et al.  Quantitative solid phase microextraction - gas chromatography mass spectrometry analysis of the pesticides lindane, heptachlor and two heptachlor transformation products in groundwater. , 2013, Journal of chromatography. A.

[30]  R. Crooks,et al.  Three-dimensional paper microfluidic devices assembled using the principles of origami. , 2011, Journal of the American Chemical Society.

[31]  Wei-Wei Zhao,et al.  The coupling of localized surface plasmon resonance-based photoelectrochemistry and nanoparticle size effect: towards novel plasmonic photoelectrochemical biosensing. , 2012, Chemical communications.

[32]  S. Shevkoplyas,et al.  Integrated separation of blood plasma from whole blood for microfluidic paper-based analytical devices. , 2012, Lab on a chip.

[33]  Shengchao Zhu,et al.  Photoelectrochemical sensor for the rapid detection of in situ DNA damage induced by enzyme-catalyzed fenton reaction. , 2008, Environmental science & technology.

[34]  Serge Cosnier,et al.  Photoelectrochemical immunosensor for label-free detection and quantification of anti-cholera toxin antibody. , 2006, Journal of the American Chemical Society.

[35]  Tae-Hyeong Kim,et al.  Paper on a disc: balancing the capillary-driven flow with a centrifugal force. , 2011, Lab on a chip.

[36]  G. Whitesides,et al.  Understanding wax printing: a simple micropatterning process for paper-based microfluidics. , 2009, Analytical chemistry.

[37]  Jinghua Yu,et al.  Three-dimensional paper-based electrochemiluminescence immunodevice for multiplexed measurement of biomarkers and point-of-care testing. , 2012, Biomaterials.

[38]  J. L. Delaney,et al.  Electrogenerated chemiluminescence detection in paper-based microfluidic sensors. , 2011, Analytical chemistry.

[39]  Catherine J. Murphy,et al.  An Improved Synthesis of High‐Aspect‐Ratio Gold Nanorods , 2003 .

[40]  John D Brennan,et al.  Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. , 2009, Analytical chemistry.

[41]  Emanuel Carrilho,et al.  Paper-based ELISA. , 2010, Angewandte Chemie.

[42]  Dan Du,et al.  Recognition of dimethoate carried by bi-layer electrodeposition of silver nanoparticles and imprinted poly-o-phenylenediamine , 2008 .

[43]  Xiaobo Chen,et al.  Titanium dioxide nanomaterials: synthesis, properties, modifications, and applications. , 2007, Chemical reviews.

[44]  Qingji Xie,et al.  Development of a new atropine sulfate bulk acoustic wave sensor based on a molecularly imprinted electrosynthesized copolymer of aniline with o-phenylenediamine , 2000 .

[45]  D. Citterio,et al.  Inkjet-printed microfluidic multianalyte chemical sensing paper. , 2008, Analytical chemistry.

[46]  Gil Garnier,et al.  Effect of polymers on the retention and aging of enzyme on bioactive papers. , 2010, Colloids and surfaces. B, Biointerfaces.

[47]  Seung-Hyeon Moon,et al.  Preparation of a highly sensitive enzyme electrode using gold nanoparticles for measurement of pesticides at the ppt level. , 2008, Journal of environmental monitoring : JEM.

[48]  Zhixian Gao,et al.  A fluoroimmunoassay based on quantum dot-streptavidin conjugate for the detection of chlorpyrifos. , 2010, Journal of agricultural and food chemistry.

[49]  Niyazi Serdar Sariciftci,et al.  Effects of Postproduction Treatment on Plastic Solar Cells , 2003 .

[50]  Shengshui Hu,et al.  Inkjet printing of nanoporous gold electrode arrays on cellulose membranes for high-sensitive paper-like electrochemical oxygen sensors using ionic liquid electrolytes. , 2012, Analytical chemistry.

[51]  Á. Maquieira,et al.  Highly sensitive enzyme-linked immunosorbent assay for chlorpyrifos. Application to olive oil analysis. , 2005, Journal of agricultural and food chemistry.

[52]  S. Ramachandran,et al.  A low cost point-of-care viscous sample preparation device for molecular diagnosis in the developing world; an example of microfluidic origami. , 2012, Lab on a chip.

[53]  Limei Tian,et al.  Paper-based SERS swab for rapid trace detection on real-world surfaces. , 2010, ACS applied materials & interfaces.

[54]  M. Bowker,et al.  New insights into the mechanism of photocatalytic reforming on Pd/TiO2 , 2011 .

[55]  T. Wen,et al.  Electrochemical copolymerization of aniline and para-phenylenediamine on IrO2-coated titanium electrode , 1994 .

[56]  Roar R. Søndergaard,et al.  Advanced materials and processes for polymer solar cell devices , 2010 .

[57]  Itamar Willner,et al.  Electrochemical, photoelectrochemical, and surface plasmon resonance detection of cocaine using supramolecular aptamer complexes and metallic or semiconductor nanoparticles. , 2009, Analytical chemistry.

[58]  Jinghong Li,et al.  Biofunctional titania nanotubes for visible-light-activated photoelectrochemical biosensing. , 2010, Analytical chemistry.

[59]  Jianping Li,et al.  A sensitive and selective sensor for dopamine determination based on a molecularly imprinted electropolymer of o-aminophenol , 2009 .

[60]  Hai-chao Liang,et al.  Visible-induced photocatalytic reactivity of polymer-sensitized titania nanotube films , 2009 .

[61]  Bingcheng Lin,et al.  Rapid prototyping of paper‐based microfluidics with wax for low‐cost, portable bioassay , 2009, Electrophoresis.