Purification and Characterization of Extracellular Lipase from Staphylococcus epidermidis (MTCC 10656)

Lipases are hydrolytic enzymes with wide range of industrial applications. In the present study Staphylococcus epidermidis strain L2 with ability to produce extracellular lipase enzyme has been isolated from cloth samples collected from the diary industry. The extracellular lipase enzyme has been purified to homogeneity. The purified enzyme has shown a specific activity of 123.95U/mg. The molecular weight of the purified lipase enzyme was found to be 28KDa. The pH and temperature optima of the enzyme were recorded as 7.5 and 40°C respectively. The lipase enzyme has shown stability and activity in presence of varying concentrations of emulsifier and different substrates.

[1]  S. Gopinath,et al.  Lipase-Secreting Bacillus Species in an Oil-Contaminated Habitat: Promising Strains to Alleviate Oil Pollution , 2015, BioMed research international.

[2]  S. S. Kanwar,et al.  Organic Solvent Tolerant Lipases and Applications , 2014, TheScientificWorldJournal.

[3]  A. Gessesse,et al.  Microbial Lipases and Their Industrial Applications: Review , 2012 .

[4]  A. Suwanto,et al.  Characterization of lipases from Staphylococcus aureus and Staphylococcus epidermidis isolated from human facial sebaceous skin. , 2012, Journal of microbiology and biotechnology.

[5]  N. R. Kamini,et al.  PROCESS STRATEGIES FOR ALKALINE LIPASE PRODUCTION USING Aspergillus Niger MTCC 2594 , 2011 .

[6]  G. Volpato,et al.  Single-step purification of different lipases from Staphylococcus warneri. , 2010, Journal of chromatography. A.

[7]  E. Park,et al.  Characterization and optimization of extracellular alkaline lipase production by Alcaligenes sp. Using stearic acid as carbon source , 2009 .

[8]  Tadeusz Antczak,et al.  Enzymatic biodiesel synthesis – Key factors affecting efficiency of the process , 2009 .

[9]  A. Gutiérrez,et al.  Microbial and enzymatic control of pitch in the pulp and paper industry , 2009, Applied Microbiology and Biotechnology.

[10]  M. Casal,et al.  Application of enzymes for textile fibres processing , 2008 .

[11]  Shu-Wei Chang,et al.  Enzymatic approach to biodiesel production. , 2007, Journal of agricultural and food chemistry.

[12]  A. Chaubey,et al.  Arthrobacter sp. lipase immobilization for improvement in stability and enantioselectivity , 2006, Applied Microbiology and Biotechnology.

[13]  G. N. Qazi,et al.  Rose Oxides: A Facile Chemo and Chemo-Enzymatic Approach. , 2006 .

[14]  A. Kademi,et al.  Lipases and their industrial applications , 2004, Applied biochemistry and biotechnology.

[15]  R. Gupta,et al.  Bacterial lipases: an overview of production, purification and biochemical properties , 2004, Applied Microbiology and Biotechnology.

[16]  T. Miyazawa,et al.  Enzymatic resolution of 2-phenoxy-1-propanols through the enantioselective acylation mediated by Achromobacter sp. lipase , 2001, Biotechnology Letters.

[17]  R. Talon,et al.  Purification and characterization of extracellular Staphylococcus warneri lipase , 2004, Current Microbiology.

[18]  P. Rathi,et al.  Lipase Mediated Upgradation of Dietary Fats and Oils , 2003, Critical reviews in food science and nutrition.

[19]  R. K. Saxena,et al.  Purification strategies for microbial lipases. , 2003, Journal of microbiological methods.

[20]  M. Lowe,et al.  The triglyceride lipases of the pancreas Published, JLR Papers in Press, October 1, 2002. DOI 10.1194/jlr.R200012-JLR200 , 2002, Journal of Lipid Research.

[21]  L. Hunter,et al.  Biochemical characterization of a recombinant thermoalkalophilic lipase and assessment of its substrate enantioselectivity , 2002 .

[22]  Torben Vedel Borchert,et al.  Industrial enzyme applications. , 2002, Current opinion in biotechnology.

[23]  U C Banerjee,et al.  Production, purification, characterization, and applications of lipases. , 2001, Biotechnology advances.

[24]  R. K. Saxena,et al.  A novel alkaline lipase from Burkholderia cepacia for detergent formulation , 2001 .

[25]  R. Verger,et al.  Effects of gum arabic on lipase interfacial binding and activity. , 2001, Analytical biochemistry.

[26]  C. Schmidt-Dannert,et al.  Gene synthesis, expression in E. coli, and in vitro refolding of Pseudomonas sp. KWI 56 and Chromobacterium viscosum lipases and their chaperones , 2001, Applied Microbiology and Biotechnology.

[27]  Shujuan Gao,et al.  Purification and characterization of a Pseudomonas sp. lipase and its properties in non‐aqueous media , 1999, Biotechnology and applied biochemistry.

[28]  S. Dharmsthiti,et al.  Production, purification and characterization of thermophilic lipase from Bacillus sp. THL027. , 1999, FEMS microbiology letters.

[29]  S. Dharmsthiti,et al.  Lipase from Pseudomonas aeruginosa LP602: biochemical properties and application for wastewater treatment , 1998, Journal of Industrial Microbiology and Biotechnology.

[30]  R K Saxena,et al.  Microbial lipases: production and applications. , 1996, Science progress.

[31]  J. Morrissey,et al.  Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. , 1981, Analytical biochemistry.

[32]  J. Troller,et al.  Isolation and characterization of a staphylococcal lipase. , 1970, Applied microbiology.

[33]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .

[34]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.