Novel fabrication technology of polydimethylsiloxane microfluidic chip

The fabrication of PDMS microfluidic chip was reported. The microchip was composed of multimode optical fiber, PDMS cover slab and PDMS base slab. The mould of cover slab was achieved by using twice exposal technology and the microfluidic chip was fabricated with casting PDMS method. The microfluidic channel with a 50μm depth and the fiber channel with a 90μm depth were formed in the PDMS cover slab, and another fiber channel with a 40μm depth were made in the PDMS base slab, so the centers of the microfluidic channel and the fiber could be located in the same plane. Several factors, which affect the characteristic of the chip, were discussed. The experiment was achieved with the fabricated chip to separate Fluorescein Isothiocyanate (FITC) and FITC-labeled amino acid mixture reagent, and the feasibility of the chip was approved.