Microfluidic hydrodynamic trapping for single cell analysis: mechanisms, methods and applications

The development of hydrodynamic-based microfluidic biochips has been increasing over the years. In this technique, the cells or particles are trapped in a particular region for single cell analysis (SCA) usually without any application of external force fields such as optical, electrical, magnetic or acoustic. There is a need to explore the insights of SCA in the cell's natural state and development of these techniques is highly essential for that study. Researchers have highlighted the vast potential field that needs to be explored to develop biochip devices to suit market/researcher demands. Hydrodynamic microfluidics facilitates the development of passive lab-on-chip applications. This review gives an account of the recent advances in this field, along with their mechanisms, methods and applications.

[1]  Jens Ducrée,et al.  Handling and analysis of cells and bioparticles on centrifugal microfluidic platforms , 2012, Expert review of molecular diagnostics.

[2]  Hongkai Wu,et al.  Centrifugation-Assisted Single-Cell Trapping in a Truncated Cone-Shaped Microwell Array Chip for the Real-Time Observation of Cellular Apoptosis. , 2015, Analytical chemistry.

[3]  Jae-Sung Park,et al.  Continuous focusing of microparticles using inertial lift force and vorticity via multi-orifice microfluidic channels. , 2009, Lab on a chip.

[4]  Sehyun Shin,et al.  Continuous separation of microparticles in a microfluidic channel via the elasto-inertial effect of non-Newtonian fluid. , 2011, Lab on a chip.

[5]  A Karimi,et al.  Hydrodynamic mechanisms of cell and particle trapping in microfluidics. , 2013, Biomicrofluidics.

[6]  Joonwon Kim,et al.  Hydrodynamic trap-and-release of single particles using dual-function elastomeric valves: design, fabrication, and characterization , 2012 .

[7]  Nicole K Henderson-Maclennan,et al.  Deformability-based cell classification and enrichment using inertial microfluidics. , 2011, Lab on a chip.

[8]  A. Manz,et al.  Micro total analysis systems. Latest advancements and trends. , 2006, Analytical chemistry.

[9]  Shuichi Shoji,et al.  High-throughput single-cell manipulation system for a large number of target cells. , 2011, Biomicrofluidics.

[10]  David K. Wood,et al.  Single cell trapping and DNA damage analysis using microwell arrays , 2010, Proceedings of the National Academy of Sciences.

[11]  Luke P. Lee,et al.  Dynamic single cell culture array. , 2006, Lab on a chip.

[12]  S. Lee,et al.  Sheathless elasto-inertial particle focusing and continuous separation in a straight rectangular microchannel. , 2011, Lab on a chip.

[13]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[14]  A. Nehorai,et al.  Finite element simulations of hydrodynamic trapping in microfluidic particle-trap array systems. , 2013, Biomicrofluidics.

[15]  Allan Guan,et al.  Streamline based design guideline for deterministic microfluidic hydrodynamic single cell traps. , 2015, Biomicrofluidics.

[16]  Arum Han,et al.  A high-throughput microfluidic single-cell screening platform capable of selective cell extraction. , 2015, Lab on a chip.

[17]  Huaying Chen,et al.  High-throughput, deterministic single cell trapping and long-term clonal cell culture in microfluidic devices. , 2015, Lab on a chip.

[18]  Mehmet E. Solmaz,et al.  Microfluidic bio-particle manipulation for biotechnology , 2014 .

[19]  Kyu Hyun,et al.  Deformability-selective particle entrainment and separation in a rectangular microchannel using medium viscoelasticity , 2012 .

[20]  Arezoo M Ardekani,et al.  Bacterial aggregation and biofilm formation in a vortical flow. , 2012, Biomicrofluidics.

[21]  Mehmet Toner,et al.  Particle focusing in staged inertial microfluidic devices for flow cytometry. , 2010, Analytical chemistry.

[22]  Donald Wlodkowic,et al.  Microfluidic single-cell array cytometry for the analysis of tumor apoptosis. , 2009, Analytical chemistry.

[23]  Min Cheol Park,et al.  High-throughput single-cell quantification using simple microwell-based cell docking and programmable time-course live-cell imaging. , 2011, Lab on a chip.

[24]  M. Packirisamy,et al.  Microfluidic positioning of pollen grains in lab-on-a-chip for single cell analysis. , 2014, Journal of bioscience and bioengineering.

[25]  Changhuei Yang,et al.  A high-efficiency microfluidic device for size-selective trapping and sorting. , 2014, Lab on a chip.

[26]  Klaus Eyer,et al.  A microchamber array for single cell isolation and analysis of intracellular biomolecules. , 2012, Lab on a chip.

[27]  Sascha Hilgenfeldt,et al.  Size-sensitive sorting of microparticles through control of flow geometry , 2011 .

[28]  Charles M Schroeder,et al.  Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution. , 2014, Lab on a chip.

[29]  K. I. Belousov,et al.  A microfluidic chip with hydrodynamic traps for in vitro microscopic investigations of single cells , 2015 .

[30]  Sascha Hilgenfeldt,et al.  Efficient manipulation of microparticles in bubble streaming flows. , 2012, Biomicrofluidics.

[31]  Charles M Schroeder,et al.  A microfluidic-based hydrodynamic trap: design and implementation. , 2011, Lab on a chip.

[32]  Doryaneh Ahmadpour,et al.  Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications , 2013, Micromachines.

[33]  Mohd Ridzuan Ahmad,et al.  Numerical Analysis of Hydrodynamic Flow in Microfluidic Biochip for Single-Cell Trapping Application , 2015, International journal of molecular sciences.

[34]  M. Tanyeri,et al.  Characterizing the performance of the hydrodynamic trap using a control-based approach , 2015 .

[35]  Nancy L Allbritton,et al.  Trapping cells on a stretchable microwell array for single-cell analysis , 2011, Analytical and Bioanalytical Chemistry.

[36]  B. Olde,et al.  Non-contact acoustic capture of microparticles from small plasma volumes. , 2015, Lab on a chip.

[37]  P. Dittrich,et al.  Current trends in single cell analysis , 2014, Analytical and Bioanalytical Chemistry.

[38]  Robert M Johann,et al.  Cell trapping in microfluidic chips , 2006, Analytical and bioanalytical chemistry.

[39]  Padmapriya,et al.  Recent Advances in Nano Patterning and Nano Imprint Lithography for Biological Applications , 2014 .

[40]  Chao Liu,et al.  A microfluidic digital single-cell assay for the evaluation of anticancer drugs , 2015, Analytical and Bioanalytical Chemistry.

[41]  A. Valero,et al.  Optimization of microfluidic single cell trapping for long-term on-chip culture. , 2010, Lab on a chip.

[42]  Rong Fan,et al.  Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells , 2011, Proceedings of the National Academy of Sciences.

[43]  Han Wei Hou,et al.  Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation. , 2011, Lab on a chip.

[44]  S. Bodovitz,et al.  Single cell analysis: the new frontier in 'omics'. , 2010, Trends in biotechnology.

[45]  A. van den Berg,et al.  High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel. , 2012, Lab on a chip.

[46]  Barry R Lutz,et al.  Hydrodynamic tweezers: 1. Noncontact trapping of single cells using steady streaming microeddies. , 2006, Analytical chemistry.

[47]  Kahp Yang Suh,et al.  Cell research with physically modified microfluidic channels: a review. , 2008, Lab on a chip.

[48]  Mengsu Yang,et al.  Microfluidics technology for manipulation and analysis of biological cells , 2006 .

[49]  Hansen Bow,et al.  Microfluidics for cell separation , 2010, Medical & Biological Engineering & Computing.

[50]  D. Gallego-Perez,et al.  Single-cell trapping and selective treatment via co-flow within a microfluidic platform. , 2014, Biosensors & bioelectronics.

[51]  T. Laurell,et al.  Review of cell and particle trapping in microfluidic systems. , 2009, Analytica chimica acta.

[52]  Pier Luca Maffettone,et al.  Single line particle focusing induced by viscoelasticity of the suspending liquid: theory, experiments and simulations to design a micropipe flow-focuser. , 2012, Lab on a chip.

[53]  Changlu Gao,et al.  Fabrication of two-layer poly(dimethyl siloxane) devices for hydrodynamic cell trapping and exocytosis measurement with integrated indium tin oxide microelectrodes arrays , 2013, Biomedical microdevices.

[54]  Ashwin Seshia,et al.  A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging , 2014, Sensors and actuators. B, Chemical.

[55]  Charles M Schroeder,et al.  Stokes trap for multiplexed particle manipulation and assembly using fluidics , 2016, Proceedings of the National Academy of Sciences.

[56]  A. Folch,et al.  Large-scale single-cell trapping and imaging using microwell arrays. , 2005, Analytical chemistry.

[57]  M. Tanyeri,et al.  Manipulation and confinement of single particles using fluid flow. , 2013, Nano letters.

[58]  A. Valizadeh,et al.  Single-cell analysis based on lab on a chip fluidic system , 2015 .

[59]  Shuichi Takayama,et al.  Single cell trapping in larger microwells capable of supporting cell spreading and proliferation , 2010, Microfluidics and nanofluidics.

[60]  D. Chen,et al.  Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping , 2014, TheScientificWorldJournal.

[61]  Susanne Braunmüller,et al.  Separation of blood cells using hydrodynamic lift , 2012 .

[62]  O. Pereira-smith,et al.  Microfluidics device for single cell gene expression analysis in Saccharomyces cerevisiae , 2006, Yeast.

[63]  D Jin,et al.  A microfluidic device enabling high-efficiency single cell trapping. , 2015, Biomicrofluidics.

[64]  Francesca Nason,et al.  Geometrical effects in microfluidic-based microarrays for rapid, efficient single-cell capture of mammalian stem cells and plant cells. , 2012, Biomicrofluidics.

[65]  Kazufumi Hosoda,et al.  Hydrodynamic trapping of Tetrahymena thermophila for the long-term monitoring of cell behaviors. , 2012, Lab on a chip.

[66]  T. Huang,et al.  Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. , 2009, Lab on a chip.

[67]  M. Tanyeri,et al.  Hydrodynamic trap for single particles and cells. , 2010, Applied physics letters.

[68]  A. Bhagat,et al.  Inertial microfluidics for continuous particle separation in spiral microchannels. , 2009, Lab on a chip.

[69]  K. Jensen,et al.  Cells on chips , 2006, Nature.