Irradiation enhances expression of cxcr4 in murine glioma cells via HIF-1α-independent pathway

Objective To investigate levels of chemokine (C–X–C motif) receptor 4 (cxcr4) mRNA and protein in X-irradiated glioma cells. Methods Murine malignant glioma GL261 cells transfected with hypoxia-inducible factor (HIF)-1α miRNA or control miRNA were irradiated with X-radiation. Cxcr4 mRNA and protein were analysed using real-time reverse transcription–polymerase chain reaction and Western blot, respectively. Results Levels of cxcr4 protein in GL261 cells increased in a radiation dose-dependent manner 48 h after 0, 5, 10 and 15 Gy X-irradiation. Irradiation of both HIF-1α knockdown cells and control cells resulted in a significant increase in cxcr4 mRNA levels, compared with nonirradiated cells, at 24 h after 5 Gy X-irradiation. Conclusion Irradiation enhances expression of cxcr4 in glioma cells via a HIF-1α-independent pathway.

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