Non‐invasive assessment of healing of bacteria infected and uninfected wounds using optical coherence tomography

Background/purpose: Bacterial infection is one of the main predisposing factors for the delay in wound healing. To facilitate a timely decision for correct therapy, it is important to accurately monitor the morphological changes in the infected wounds using noninvasive tools. In the present study, we have explored the use of optical coherence tomography (OCT) for monitoring the healing of superficial wounds infected with Staphylococcus aureus in mice under in vitro and in vivo conditions and studied the changes in collagen birefringence in the infected wounds.

[1]  G. Dubin,et al.  Staphylococcus aureus-Derived Staphopain B, a Potent Cysteine Protease Activator of Plasma Chemerin1 , 2007, The Journal of Immunology.

[2]  A Gh Podoleanu,et al.  Optical coherence tomography. , 2005, The British journal of radiology.

[3]  George Broughton,et al.  The Basic Science of Wound Healing , 2006, Plastic and reconstructive surgery.

[4]  J. Cuzzell,et al.  The new RYB color code. , 1988, The American journal of nursing.

[5]  A. Nerlich,et al.  The time-dependent rearrangement of the epithelial basement membrane in human skin wounds —immunohistochemical localization of Collagen IV and VII , 2006, International Journal of Legal Medicine.

[6]  D. Greenhalgh,et al.  Cutaneous Wound Healing , 2007, Journal of burn care & research : official publication of the American Burn Association.

[7]  P. Kovanen,et al.  Activation of interstitial collagenase, MMP‐1, by Staphylococcus aureus cells having surface‐bound plasmin: a novel role of plasminogen receptors of bacteria , 1999, FEBS letters.

[8]  M. Bokarewa,et al.  Staphylococcus aureus: Staphylokinase. , 2006, The international journal of biochemistry & cell biology.

[9]  T. Milner,et al.  Review of polarization sensitive optical coherence tomography and Stokes vector determination. , 2002, Journal of biomedical optics.

[10]  T. K. Hunt,et al.  Noninvasive assessment of wound-healing angiogenesis with contrast-enhanced MRI. , 2002, Academic radiology.

[11]  A. Hasegawa,et al.  Effects of staphylococci on cytokine production from human keratinocytes , 2003, The British journal of dermatology.

[12]  Y. Verma,et al.  Effect of Helium-Neon Laser Irradiation on Hair Follicle Growth Cycle of Swiss Albino Mice , 2009, Skin Pharmacology and Physiology.

[13]  T. Magee,et al.  Methicillin resistant Staphylococcus aureus in patients undergoing major amputation. , 2001, European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery.

[14]  D. Andersson,et al.  Establishment of a Superficial Skin Infection Model in Mice by Using Staphylococcus aureus and Streptococcus pyogenes , 2005, Antimicrobial Agents and Chemotherapy.

[15]  L. Hughes.  Wound measurement. , 1983, Canadian journal of surgery. Journal canadien de chirurgie.

[16]  Takashi Oono,et al.  Staphylococcal cutaneous infections: invasion, evasion and aggression. , 2006, Journal of dermatological science.

[17]  Shanmugam Kirubanandan,et al.  Triphala promotes healing of infected full-thickness dermal wound. , 2008, The Journal of surgical research.

[18]  J. Travers,et al.  The keratinocyte as a target for staphylococcal bacterial toxins. , 2001, The journal of investigative dermatology. Symposium proceedings.

[19]  D. Keast,et al.  MEASURE: A proposed assessment framework for developing best practice recommendations for wound assessment. , 2004, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[20]  J. Deringer,et al.  Apoptosis Induced by Staphylococcus aureus in Epithelial Cells Utilizes a Mechanism Involving Caspases 8 and 3 , 2000, Infection and Immunity.

[21]  S. Foster,et al.  Peptidoglycan and Lipoteichoic Acid from Staphylococcus aureus Induce Tumor Necrosis Factor Alpha, Interleukin 6 (IL-6), and IL-10 Production in Both T Cells and Monocytes in a Human Whole Blood Model , 2000, Infection and Immunity.

[22]  Yingtian Pan,et al.  Optical coherence tomography: a noninvasive method to assess wound reepithelialization. , 2007, Academic emergency medicine : official journal of the Society for Academic Emergency Medicine.

[23]  W M Petroll,et al.  Specular Microscopy, Confocal Microscopy, and Ultrasound Biomicroscopy: Diagnostic Tools of the Past Quarter Century , 2000, Cornea.

[24]  Phillip Blondeel,et al.  Assessment of burn depth and burn wound healing potential. , 2008, Burns : journal of the International Society for Burn Injuries.

[25]  T. Brosh,et al.  The effect of bacterial endotoxin on the early tensile strength of healing surgical wounds. , 2002, Journal of endodontics.

[26]  Changes of human skin in subepidermal wound healing process , 2008, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[27]  K Divakar Rao,et al.  Real‐time in vivo imaging of adult Zebrafish brain using optical coherence tomography , 2009, Journal of biophotonics.

[28]  G. Opdenakker,et al.  Matrix metalloproteinase-9 (gelatinase B) deficiency leads to increased severity of Staphylococcus aureus-triggered septic arthritis. , 2006, Microbes and infection.

[29]  Pei-Lin Hsiung,et al.  Effect of tissue preservation on imaging using ultrahigh resolution optical coherence tomography. , 2005, Journal of biomedical optics.

[30]  Thilo Gambichler,et al.  Applications of optical coherence tomography in dermatology. , 2005, Journal of dermatological science.

[31]  W. Carter,et al.  Morphological evidence for the role of suprabasal keratinocytes in wound reepithelialization , 2005, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[32]  J. Welzel.  Optical coherence tomography in dermatology: a review , 2001, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[33]  Xingde Li,et al.  Noninvasive assessment of cutaneous wound healing using ultrahigh-resolution optical coherence tomography. , 2006, Journal of biomedical optics.

[34]  Jie Li,et al.  Pathophysiology of acute wound healing. , 2007, Clinics in dermatology.

[35]  Ruikang K. Wang,et al.  Spectral domain polarization sensitive optical coherence tomography achieved by single camera detection. , 2007, Optics express.

[36]  N. Cook,et al.  Methicillin-resistant Staphylococcus aureus versus the burn patient. , 1998, Burns : journal of the International Society for Burn Injuries.

[37]  R. Saadia.  Surgical Wound Healing and Management , 2007, World Journal of Surgery.

[38]  Beop-Min Kim,et al.  Quantification of the wound healing using polarization-sensitive optical coherence tomography. , 2006, Journal of biomedical optics.

[39]  F A Matsen,et al.  A model for the study of wounds in normal elderly adults and patients with peripheral vascular disease or diabetes mellitus. , 1995, The Journal of surgical research.