Smartphone-based colorimetric analysis for detection of saliva alcohol concentration.

A simple device and associated analytical methods are reported. We provide objective and accurate determination of saliva alcohol concentrations using smartphone-based colorimetric imaging. The device utilizes any smartphone with a miniature attachment that positions the sample and provides constant illumination for sample imaging. Analyses of histograms based on channel imaging of red-green-blue (RGB) and hue-saturation-value (HSV) color space provide unambiguous determination of blood alcohol concentration from color changes on sample pads. A smartphone-based sample analysis by colorimetry was developed and tested with blind samples that matched with the training sets. This technology can be adapted to any smartphone and used to conduct color change assays.

[1]  R D Schmid,et al.  Development of a gas diffusion FIA system for on-line monitoring of ethanol. , 1990, Journal of biotechnology.

[2]  Aydogan Ozcan,et al.  Cellphone-based devices for bioanalytical sciences , 2014, Analytical and Bioanalytical Chemistry.

[3]  K. Inoue,et al.  Evaluation of L-pyrrolidonyl peptidase paper strip test for differentiation of members of the family Enterobacteriaceae, particularly Salmonella spp , 1996, Journal of clinical microbiology.

[4]  Hongying Zhu,et al.  Cost-effective and rapid blood analysis on a cell-phone. , 2013, Lab on a chip.

[5]  Huan‐Tsung Chang,et al.  Detection of mercury(II) ions using colorimetric gold nanoparticles on paper-based analytical devices. , 2014, Analytical chemistry.

[6]  P. Kaufmann,et al.  Q.E.D. Alcohol Test: a simple and quick method to detect ethanol in saliva of patients in emergency departments , 1999, Intensive Care Medicine.

[7]  Ali K. Yetisen,et al.  A smartphone algorithm with inter-phone repeatability for the analysis of colorimetric tests , 2014 .

[8]  M. Bates,et al.  Immediate, quantitative estimation of blood alcohol concentration from saliva. , 1997, Journal of studies on alcohol.

[9]  Dariusz Zuba,et al.  Saliva as an alternative specimen for alcohol determination in the human body. , 2002, Polish journal of pharmacology.

[10]  A. Roda,et al.  Integrating biochemiluminescence detection on smartphones: mobile chemistry platform for point-of-need analysis. , 2014, Analytical chemistry.

[11]  Derek Tseng,et al.  Fluorescent imaging of single nanoparticles and viruses on a smart phone. , 2013, ACS nano.

[12]  David J. You,et al.  Cell-phone-based measurement of TSH using Mie scatter optimized lateral flow assays. , 2013, Biosensors & bioelectronics.

[13]  Amy L. Gryshuk,et al.  Cell-Phone-Based Platform for Biomedical Device Development and Education Applications , 2011, PloS one.

[14]  Yi Xiao,et al.  Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes , 2010, Proceedings of the National Academy of Sciences.

[15]  Chao-Min Cheng,et al.  Paper - a potential platform in pharmaceutical development. , 2015, Trends in biotechnology.

[16]  Kori L Brewer,et al.  An alcohol oxidase dipstick rapidly detects methanol in the serum of mice. , 2007, Academic emergency medicine : official journal of the Society for Academic Emergency Medicine.

[17]  P. Nath,et al.  Label-free biodetection using a smartphone. , 2013, Lab on a chip.

[18]  Wenyue Li,et al.  Smartphone quantifies Salmonella from paper microfluidics. , 2013, Lab on a chip.

[19]  R. Schwartz,et al.  Evaluation of colorimetric dipstick test to detect alcohol in saliva: a pilot study. , 1989, Annals of emergency medicine.

[20]  Daniel A. Fletcher,et al.  Low-Cost Mobile Phone Microscopy with a Reversed Mobile Phone Camera Lens , 2014, PloS one.

[21]  Debjani Paul,et al.  A paperfluidic device for dental applications using a novel patterning technique , 2015 .