Sequence, expression and evolutionary relationships of carbamoyl phosphate synthetase I in the toad Xenopus laevis.

The sequence of carbamoyl phosphate synthetase I (CPSase I) cDNA and expression of the enzyme in liver of the toad Xenopus laevis are reported. CPSase I mRNA increases 6-fold when toads are exposed to high salinity for extended periods of time. The deduced 1,494-amino acid sequence of the CPSase I is homologous to other CPSases and reveals a domain structure and conserved amino acids common to other CPSases. A serine residue (S287) is present where there is a cysteine residue required for glutamine-dependent activity in CPSase Types III and II (Type I CPSases utilize only ammonia as nitrogen-donating substrate). A sequence of DNA 964 bases upstream from the ATG start codon for the CPSase I gene is also reported. Phylogenetic analysis for 30 CPSase isoforms, including X. laevis CPSase I, across a wide spectrum of phyla is reported and discussed. The results are consistent with the views that eukaryotic CPSase II as a multifunctional complex evolved from prokaryotic CPSase II and that CPSase I in terrestrial vertebrates and CPSase III in fishes arose from eukaryotic CPSase II by independent events after the divergence of plants in eukaryotic evolution.

[1]  P. Anderson,et al.  The promoter region of the carbamoyl-phosphate synthetase III gene ofSqualus acanthias , 1996, Journal of Molecular Evolution.

[2]  W. Lamers,et al.  Evolutionary relationships of the carbamoylphosphate synthetase genes , 1995, Journal of Molecular Evolution.

[3]  R. W. Griffith.  Guppies, toadfish, lungfish, coelacanths and frogs: a scenario for the evolution of urea retention in fishes , 1991, Environmental Biology of Fishes.

[4]  D. Hillis,et al.  The relationships of the coelacanth Latimeria chalumnae: evidence from sequences of vertebrate 28S ribosomal RNA genes , 1991, Environmental Biology of Fishes.

[5]  K. Hiong,et al.  Urea synthesis in the African lungfish Protopterus dolloi - hepatic carbamoyl phosphate synthetase III and glutamine synthetase are upregulated by 6 days of aerial exposure , 2003, Journal of Experimental Biology.

[6]  S. Powers-Lee,et al.  Gain of Glutaminase Function in Mutants of the Ammonia-specific Frog Carbamoyl Phosphate Synthetase* , 2003, Journal of Biological Chemistry.

[7]  Judith A. Blake,et al.  MGD: the Mouse Genome Database , 2003, Nucleic Acids Res..

[8]  André Johann,et al.  Updating Carbamoylphosphate Synthase (CPS) Phylogenies: Occurrence and Phylogenetic Identity of Archaeal CPS Genes , 2002, Journal of Molecular Evolution.

[9]  D. Swofford.  PAUP*: Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10 , 2002 .

[10]  Sudhir Kumar,et al.  MEGA2: molecular evolutionary genetics analysis software , 2001, Bioinform..

[11]  P. Anderson.  Urea and glutamine synthesis: Environmental influences on nitrogen excretion , 2001 .

[12]  B. F. Terjesen,et al.  Detection and basic properties of carbamoyl phosphate synthetase III during teleost ontogeny: a case study in the Atlantic halibut (Hippoglossus hippoglossus L.). , 2000, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[13]  B. Hyman,et al.  Alfalfa (Medicago sativa) carbamoylphosphate synthetase gene structure records the deep lineage of plants. , 2000, Gene.

[14]  P. Walsh,et al.  Induction of carbamoyl phosphate synthetase III and glutamine synthetase mRNA during confinement stress in gulf toadfish (Opsanus beta). , 2000, The Journal of experimental biology.

[15]  C. Wood,et al.  Muscle as the Primary Site of Urea Cycle Enzyme Activity in an Alkaline Lake-adapted Tilapia, Oreochromis alcalicus grahami * , 1999, The Journal of Biological Chemistry.

[16]  T. Aoki,et al.  Carbamoyl-phosphate synthetase II in kinetoplastids. , 1998, Biochimica et biophysica acta.

[17]  N. Saha,et al.  Ureogenesis in Indian air-breathing teleosts: adaptation to environmental constraints , 1998 .

[18]  J. Korte,et al.  Nitrogen excretion and expression of carbamoyl-phosphate synthetase III activity and mRNA in extrahepatic tissues of largemouth bass (Micropterus salmoides). , 1998, Archives of biochemistry and biophysics.

[19]  P. Anderson,et al.  Expression and Activity of Carbamoyl Phosphate Synthetase III and Ornithine Urea Cycle Enzymes in Various Tissues of Four Fish Species , 1998 .

[20]  C. Jørgensen,et al.  Urea and amphibian water economy. , 1997, Comparative biochemistry and physiology. Part A, Physiology.

[21]  I. Rayment,et al.  Structure of carbamoyl phosphate synthetase: a journey of 96 A from substrate to product. , 1997, Biochemistry.

[22]  V. Cabrera,et al.  Expression of Carbamoyl-phosphate Synthetase III mRNA during the Early Stages of Development and in Muscle of Adult Rainbow Trout (Oncorhynchus mykiss)* , 1997, The Journal of Biological Chemistry.

[23]  P. Anderson,et al.  Carbamyl Phosphate Synthetases in an Air-Breathing Teleost, Heteropneustes fossilis , 1997 .

[24]  R. L. Charlebois,et al.  Phylogenetic analysis of carbamoylphosphate synthetase genes: complex evolutionary history includes an internal duplication within a gene which can root the tree of life. , 1996, Molecular biology and evolution.

[25]  P. Anderson.  3 Urea Cycle in Fish: Molecular and Mitochondrial Studies , 1995 .

[26]  Wright.,et al.  Induction of ornithine-urea cycle enzymes and nitrogen metabolism and excretion in rainbow trout (Oncorhynchus mykiss) during early life stages , 1995, The Journal of experimental biology.

[27]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[28]  C. J. Lusty,et al.  Carbamyl phosphate synthetase III, an evolutionary intermediate in the transition between glutamine-dependent and ammonia-dependent carbamyl phosphate synthetases. , 1994, Journal of molecular biology.

[29]  C. Helbing,et al.  3,5,3'-Triiodothyronine-induced carbamyl-phosphate synthetase gene expression is stabilized in the liver of Rana catesbeiana tadpoles during heat shock. , 1994, The Journal of biological chemistry.

[30]  K. Chen,et al.  The evolutionary history of the first three enzymes in pyrimidine biosynthesis , 1993, BioEssays : news and reviews in molecular, cellular and developmental biology.

[31]  J. Schofield.  Molecular studies on an ancient gene encoding for carbamoyl-phosphate synthetase. , 1993, Clinical science.

[32]  P. Anderson,et al.  Evolution of mitochondrial enzyme systems in fish: the mitochondrial synthesis of glutamine and citrulline , 1991 .

[33]  D. Evans,et al.  Mammalian carbamyl phosphate synthetase (CPS). DNA sequence and evolution of the CPS domain of the Syrian hamster multifunctional protein CAD. , 1990, The Journal of biological chemistry.

[34]  M. Takiguchi,et al.  Evolutionary aspects of urea cycle enzyme genes , 1989, BioEssays : news and reviews in molecular, cellular and developmental biology.

[35]  A. Jagendorf,et al.  Site of Synthesis of the Enzymes of the Pyrimidine Biosynthetic Pathway in Oat (Avena sativa L.) Leaves. , 1987, Plant physiology.

[36]  H. Ochiai,et al.  Localization of carbamoylphosphate synthetase and aspartate carbamoyltransferase in chloroplasts. , 1986, Plant physiology.

[37]  C. J. Lusty,et al.  Characterization and derivation of the gene coding for mitochondrial carbamyl phosphate synthetase I of rat. , 1985, The Journal of biological chemistry.

[38]  P. Anderson.  Glutamine- and N-acetylglutamate-dependent carbamoyl phosphate synthetase in elasmobranchs. , 1980, Science.

[39]  P. Anderson.  A glutamine- and N-acetyl-L-glutamate-dependent carbamyl phosphate synthetase activity in the teleost Micropterus salmoides. , 1976, Comparative biochemistry and physiology. B, Comparative biochemistry.

[40]  P. Janssens.  The influence of ammonia on the transition to ureotelism in Xenopus laevis , 1972 .

[41]  James W. Campbell,et al.  Carbamyl phosphate synthesis in invertebrates , 1971 .

[42]  J. W. Campbell,et al.  Carbamyl phosphate synthesis in a land snail, Strophocheilus oblongus. , 1970, The Journal of biological chemistry.

[43]  L. Goldstein,et al.  Accelerated synthesis of urea in Xenopus laevis during osmotic stress. , 1970, The American journal of physiology.

[44]  L. Goldstein,et al.  Renal function during osmotic stress in the aquatic toad Xenopus laevis. , 1970, The American journal of physiology.

[45]  P. Cohen,et al.  Biosynthesis of urea in the estivating African lungfish and in Xenopus laevis under conditions of water-shortage. , 1968, Comparative biochemistry and physiology.

[46]  L. Goldstein,et al.  Ornithine-Urea Cycle Activity in Xenopus laevis: Adaptation in Saline , 1967, Science.

[47]  C. Coe,et al.  Amino acid metabolism and urea synthesis in naturally aestivating Xenopus laevis. , 1967, Comparative biochemistry and physiology.