Detection of cardiac allograft rejection by real‐time PCR analysis of circulating mononuclear cells

Abstract:  Background:  Detection of cardiac allograft rejection is based on the histological examination of endomyocardial biopsies (EMB). We have explored the possibility of whether graft rejection could be detected by characteristic gene expression patterns in peripheral blood mononuclear cells (PBMC) of heart‐transplant recipients.

[1]  P. Kimball,et al.  The paradox of cytokine monitoring-predictor of immunologic activity as well as immunologic silence following cardiac transplantation. , 1996, Transplantation.

[2]  M. Suthanthiran,et al.  Noninvasive diagnosis of renal-allograft rejection by measurement of messenger RNA for perforin and granzyme B in urine. , 2001, The New England journal of medicine.

[3]  S. Pfeffer,et al.  Vesicle traffic: Get your coat! , 1997, Current Biology.

[4]  P. McCarthy,et al.  Chemokine and chemokine receptor gene expression indicates acute rejection of human cardiac transplants1 , 2003, Transplantation.

[5]  A. Gaber,et al.  Quantitative detection of T-cell activation markers by real-time PCR in renal transplant rejection and correlation with histopathologic evaluation1 , 2002, Transplantation.

[6]  C. Nast,et al.  TGF-β in Renal Allograft Rejection , 1998, Mineral and Electrolyte Metabolism.

[7]  R. Michler,et al.  Upregulation of COX-2 during cardiac allograft rejection. , 2000, Circulation.

[8]  T. Giese.  Development of Quantitative RT-PCR Tests for the Expression of Cytokine Genes on the LightCycler , 2001 .

[9]  W. R. Lamb,et al.  Serum cytokines in human heart transplant recipients. Is there a relationship to rejection? , 1996, Transplantation.

[10]  G. McMahon,et al.  Expression of the Chemokine Receptor CXCR3 and Its Ligand IP-10 During Human Cardiac Allograft Rejection , 2001 .

[11]  C. Mavroudis,et al.  Serum vascular endothelial growth factor as a surveillance marker for cellular rejection in pediatric cardiac transplantation. , 2002, Transplantation.