The function of myosin and myosin light chain kinase phosphorylation in smooth muscle.

early physiological and pha rma cologi cal studies on smooth muscle prepara­ tions foc used primarily on extra cellular eff ectors and their interactions with the membrane components ultimately involved in the control of intra cellula r Ca2+ concentration. Eva luation of the steady-state contractile resp onse has been interpreted as an index of sarcoplasmic Ca2+ concentra tion, with the under­ lying assumption that Ca2+ activates contractile proteins through a simple switch -like mechanis m. The discoveries that the 20,OOO-dalton light chain of smooth muscle myosin could be phosphorylated (1), and that the phosphoryla­ tion of smooth muscle myosin is ass ociated with an increase in its actin­ activated

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