A microelectrode array for real-time neurochemical and neuroelectrical recording in vitro

The development of a silicon neural recording device capable of measuring neurochemical and neuroelectrical activity in tissue culture is described. It consists of a microfabricated array of platinum and chloridized silver electrodes on a silicon substrate. A culture chamber affixed to the surface of the device contains the culture medium and cells. Neuron cultures were viable for over 75 days in the devices, generating both neuroelectrical and neurochemical data in real-time. The resolution and speed of the device allows for the study of single neuron interactions with the ability to correlate electrical signals directly with chemical responses.