Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana.

The p38 mitogen-activated protein kinase (MAPK) is involved in various processes, including stress responses, development, and differentiation. However, little information on p38 MAPK in insects is available. In this study, a p38 MAPK gene, Accp38b, was isolated from Apis cerana cerana and characterized. The quantitative real-time PCR (Q-PCR) analysis revealed that Accp38b was induced by multiple stressors. Notably, the expression of Accp38b was relatively higher in the pupae phase than in other developmental phases. During the pupae phase, Accp38b expression was higher in the thorax than in the head and abdomen and higher in the fat body than in the muscle and midgut. Immunohistochemisty showed significant positive staining of Accp38b in sections from the brain, eyes, fat body, and midgut of A. cerana cerana. These results suggest that Accp38b may play a crucial role in stress responses and have multiple aspects function during development.

[1]  Baohua Xu,et al.  Characterization of the TAK1 gene in Apis cerana cerana (AccTAK1) and its involvement in the regulation of tissue-specific development. , 2011, BMB reports.

[2]  W. Yarbrough,et al.  LZAP Inhibits p38 MAPK (p38) Phosphorylation and Activity by Facilitating p38 Association with the Wild-Type p53 Induced Phosphatase 1 (WIP1) , 2011, PloS one.

[3]  P. N. Sharma,et al.  Molecular cloning, transcriptional regulation, and differential expression profiling of vitellogenin in two wing‐morphs of the brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae) , 2010, Insect molecular biology.

[4]  Xiurong Wu,et al.  Participation of the p38 pathway in Drosophila host defense against pathogenic bacteria and fungi , 2010, Proceedings of the National Academy of Sciences.

[5]  Yong Jiang,et al.  Mechanisms regulating the nuclear translocation of p38 MAP kinase , 2010, Journal of cellular biochemistry.

[6]  M. Yamaguchi,et al.  Regulation of the Drosophila p38b gene by transcription factor DREF in the adult midgut. , 2010, Biochimica et biophysica acta.

[7]  Joung-Sun Park,et al.  The role of p38b MAPK in age-related modulation of intestinal stem cell proliferation and differentiation in Drosophila , 2009, Aging.

[8]  L. J. Eldik,et al.  The p38α mitogen-activated protein kinase as a central nervous system drug discovery target , 2008, BMC Neuroscience.

[9]  R. Hodgetts,et al.  A Member of the p38 Mitogen-Activated Protein Kinase Family Is Responsible for Transcriptional Induction of Dopa decarboxylase in the Epidermis of Drosophila melanogaster during the Innate Immune Response , 2008, Molecular and Cellular Biology.

[10]  R. Leal,et al.  The activation of ERK1/2 and p38 mitogen-activated protein kinases is dynamically regulated in the developing rat visual system , 2008, International Journal of Developmental Neuroscience.

[11]  M. Yamaguchi,et al.  The DRE/DREF transcriptional regulatory system: a master key for cell proliferation. , 2008, Biochimica et biophysica acta.

[12]  T. Endo,et al.  Involvement of cAMP response element-binding protein in the regulation of cell proliferation and the prolactin promoter of lactotrophs in primary culture. , 2007, American journal of physiology. Endocrinology and metabolism.

[13]  Wei Chen,et al.  Differential regulation and properties of MAPKs , 2007, Oncogene.

[14]  R. Ueda,et al.  ATF-2 regulates fat metabolism in Drosophila. , 2007, Molecular biology of the cell.

[15]  E. Nestler,et al.  The many faces of CREB , 2005, Trends in Neurosciences.

[16]  Y. Ip,et al.  A Drosophila p38 orthologue is required for environmental stress responses , 2004, EMBO reports.

[17]  J. Blenis,et al.  ERK and p38 MAPK-Activated Protein Kinases: a Family of Protein Kinases with Diverse Biological Functions , 2004, Microbiology and Molecular Biology Reviews.

[18]  S. Park,et al.  Transcriptional regulation of the Drosophila catalase gene by the DRE/DREF system. , 2004, Nucleic acids research.

[19]  H. Ichijo,et al.  Neuronal p38 MAPK signalling: an emerging regulator of cell fate and function in the nervous system , 2002, Genes to cells : devoted to molecular & cellular mechanisms.

[20]  K. Irie,et al.  p38 Mitogen-Activated Protein Kinase Can Be Involved in Transforming Growth Factor β Superfamily Signal Transduction in Drosophila Wing Morphogenesis , 1999, Molecular and Cellular Biology.

[21]  Y. Ip,et al.  A Conserved p38 Mitogen-Activated Protein Kinase Pathway Regulates Drosophila Immunity Gene Expression , 1998, Molecular and Cellular Biology.

[22]  Kang-Yell Choi,et al.  Molecular Cloning and Characterization of aDrosophila p38 Mitogen-activated Protein Kinase* , 1998, The Journal of Biological Chemistry.

[23]  L Bibbs,et al.  A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. , 1994, Science.

[24]  S. Gill,et al.  The mitogen-activated protein kinase p38 is involved in insect defense against Cry toxins from Bacillus thuringiensis. , 2010, Insect biochemistry and molecular biology.

[25]  TylerZARUBIN,et al.  Activation and signaling of the p38 MAP kinase pathway , 2005 .

[26]  Jiahuai Han,et al.  The p38 signal transduction pathway: activation and function. , 2000, Cellular signalling.