in Systemic Lupus Erythematosus Autoantibody Formation and Organ Damage Accelerated Macrophage Apoptosis Induces

Increased monocyte/macrophage (M (cid:1) ) apoptosis occurs in patients with systemic lupus erythematosus (SLE) and is mediated, at least in part, by an autoreactive CD4 (cid:2) T cell subset. Furthermore, autoreactive murine CD4 (cid:2) T cells that kill syngeneic M (cid:1) in vitro induce a lupus-like disease in vivo. However, it is unclear whether increased M (cid:1) apoptosis in SLE per se is sufficient to accelerate/promote autoimmunity. We have investigated whether increased M (cid:1) apoptosis in vivo, induced by the administration of clodronate liposomes, can exacerbate the autoimmune phenotype in NZB (cid:3) SWR (SNF 1 ) lupus-prone mice, and induce autoantibody production in haplotype-matched BALB/c (cid:3) DBA1 (DBF 1 ) non-lupus-prone mice. Lupus-prone mice SNF 1 mice that were treated with clodronate liposomes, but not mice treated with vehicle, developed significant increases in autoantibodies to dsDNA, nucleosomes, and the idiotypically related family of nephritic Abs Id LN F 1 , when compared with untreated SNF 1 mice. Furthermore, clodronate treatment hastened the onset of proteinuria and worsened SNF 1 lupus nephritis. When compared with vehicle-treated controls, clodronate-treated non-lupus-prone DBF 1 mice developed significantly higher levels of anti-nucleosome and Id LN F 1 Abs but did not develop lupus nephritis. We propose that M (cid:1) apoptosis contributes to the pathogenesis of autoantibody formation and organ damage through both an increase in the apoptotic load and impairment in the clearance of apoptotic material. This study suggests that mechanisms that induce scavenger cell apoptosis, such as death induced by autoreactive cytotoxic T cells observed in SLE, could play a pathogenic role and contribute to the severity of the disease. The Journal of Immunology, 2006, 176: 2095–2104.

[1]  S. Hart,et al.  Phagocytosis of apoptotic cells. , 2008, Methods.

[2]  J. Witztum,et al.  Apoptotic Cells with Oxidation-specific Epitopes Are Immunogenic and Proinflammatory , 2004, The Journal of experimental medicine.

[3]  A. Hagenbeek,et al.  Enhanced engraftment of human cells in RAG2/gammac double-knockout mice after treatment with CL2MDP liposomes. , 2004, Experimental hematology.

[4]  S. Nagata,et al.  Autoimmune Disease and Impaired Uptake of Apoptotic Cells in MFG-E8-Deficient Mice , 2004, Science.

[5]  Mariana J. Kaplan,et al.  Demethylation of Promoter Regulatory Elements Contributes to Perforin Overexpression in CD4+ Lupus T Cells1 , 2004, The Journal of Immunology.

[6]  E. Baldissera,et al.  Accumulation of plasma nucleosomes upon treatment with anti‐tumour necrosis factor‐α antibodies , 2004 .

[7]  G. Sturfelt,et al.  Induction of apoptosis in monocytes and lymphocytes by serum from patients with systemic lupus erythematosus − an additional mechanism to increased autoantigen load? , 2004, Clinical and experimental immunology.

[8]  G. Boivin,et al.  An ACE inhibitor reduces Th2 cytokines and TGF-β1 and TGF-β2 isoforms in murine lupus nephritis , 2004 .

[9]  G. Zandman-Goddard,et al.  [Apoptosis in systemic lupus erythematosus]. , 2003, Harefuah.

[10]  C. Lau,et al.  Increased apoptotic neutrophils and macrophages and impaired macrophage phagocytic clearance of apoptotic neutrophils in systemic lupus erythematosus. , 2003, Arthritis and rheumatism.

[11]  D. Pisetsky,et al.  Role of macrophages in the generation of circulating blood nucleosomes from dead and dying cells. , 2003, Blood.

[12]  I. Chaudry,et al.  Inhibition of Fas/Fas ligand signaling improves septic survival: differential effects on macrophage apoptotic and functional capacity , 2003, Journal of leukocyte biology.

[13]  A. Lacy-Hulbert,et al.  Apoptotic Cells and Innate Immune Stimuli Combine to Regulate Macrophage Cytokine Secretion 1 , 2003, The Journal of Immunology.

[14]  S. Kaufmann,et al.  Macrophages of the Splenic Marginal Zone Are Essential for Trapping of Blood-Borne Particulate Antigen but Dispensable for Induction of Specific T Cell Responses , 2003, The Journal of Immunology.

[15]  C. Mohan,et al.  Use of a novel elution regimen reveals the dominance of polyreactive antinuclear autoantibodies in lupus kidneys. , 2003, Arthritis and rheumatism.

[16]  B. Weill,et al.  B cell apoptosis accelerates the onset of murine lupus , 2003, European journal of immunology.

[17]  J. Bach,et al.  Concomitant early appearance of anti-ribonucleoprotein and anti-nucleosome antibodies in lupus prone mice. , 2003, Journal of autoimmunity.

[18]  Y. Tsai,et al.  Anti‐SSB/La is one of the antineutrophil autoantibodies responsible for neutropenia and functional impairment of polymorphonuclear neutrophils in patients with systemic lupus erythematosus , 2003, Clinical and experimental immunology.

[19]  P. Marrack,et al.  Liposomal clodronate as a novel agent for treating autoimmune hemolytic anemia in a mouse model. , 2003, Blood.

[20]  A. van Nieuwenhuijze,et al.  Time between onset of apoptosis and release of nucleosomes from apoptotic cells: putative implications for systemic lupus erythematosus , 2003, Annals of the rheumatic diseases.

[21]  W. Mccune,et al.  The Apoptotic Ligands TRAIL, TWEAK, and Fas Ligand Mediate Monocyte Death Induced by Autologous Lupus T Cells1 , 2002, The Journal of Immunology.

[22]  M. Stoll,et al.  Molecular identification of pathogenetic IdLNF+1 autoantibody idiotypes derived from the NZBxSWR F1 model for systemic lupus erythematosus. , 2002, Journal of autoimmunity.

[23]  J. Charles Jennette,et al.  Delayed Apoptotic Cell Clearance and Lupus-like Autoimmunity in Mice Lacking the c-mer Membrane Tyrosine Kinase , 2002, The Journal of experimental medicine.

[24]  N. Van Rooijen,et al.  CLODRONATE LIPOSOMES: PERSPECTIVES IN RESEARCH AND THERAPEUTICS , 2002, Journal of liposome research.

[25]  Virginia Pascual,et al.  Induction of Dendritic Cell Differentiation by IFN-α in Systemic Lupus Erythematosus , 2001, Science.

[26]  Y. Shoshan,et al.  Accelerated Fas-Mediated Apoptosis of Monocytes and Maturing Macrophages from Patients with Systemic Lupus Erythematosus: Relevance to In Vitro Impairment of Interaction with iC3b-Opsonized Apoptotic Cells1 , 2001, The Journal of Immunology.

[27]  S. Kalled,et al.  Apoptosis and Altered Dendritic Cell Homeostasis in Lupus Nephritis Are Limited by Anti-CD154 Treatment , 2001, The Journal of Immunology.

[28]  M. Hyttinen,et al.  Effects of low-dose, noncytotoxic, intraarticular liposomal clodronate on development of erosions and proteoglycan loss in established antigen-induced arthritis in rabbits. , 2001, Arthritis and rheumatism.

[29]  M. Kaplan,et al.  Autoreactive murine Th1 and Th2 cells kill syngeneic macrophages and induce autoantibodies , 2001, Lupus.

[30]  L. Rönnblom,et al.  Activation of natural interferon-alpha producing cells by apoptotic U937 cells combined with lupus IgG and its regulation by cytokines. , 2001, Journal of autoimmunity.

[31]  U. Pleyer,et al.  Macrophage depletion prevents leukocyte adhesion and disease induction in experimental melanin-protein induced uveitis. , 2001, Experimental eye research.

[32]  M. Isturiz,et al.  Treatment with liposome-encapsulated clodronate as a new strategic approach in the management of immune thrombocytopenic purpura in a mouse model. , 2000, Blood.

[33]  J. Beijnen,et al.  Synovial macrophage depletion with clodronate-containing liposomes in rheumatoid arthritis. , 2000, Arthritis and rheumatism.

[34]  C. Rugarli,et al.  Remnants of suicidal cells fostering systemic autoaggression. Apoptosis in the origin and maintenance of autoimmunity. , 2000, Arthritis and rheumatism.

[35]  T. Tumpey,et al.  Role of macrophages in restricting herpes simplex virus type 1 growth after ocular infection. , 2000, Investigative ophthalmology & visual science.

[36]  M. Kaplan,et al.  TRAIL (Apo2 Ligand) and TWEAK (Apo3 Ligand) Mediate CD4+ T Cell Killing of Antigen-Presenting Macrophages1 , 2000, The Journal of Immunology.

[37]  A. Rosen,et al.  Cleavage by Granzyme B Is Strongly Predictive of Autoantigen Status , 1999, The Journal of experimental medicine.

[38]  J. Piette,et al.  The key role of nucleosomes in lupus. , 1999, Arthritis and rheumatism.

[39]  R. Steinman,et al.  Efficient Presentation of Phagocytosed Cellular Fragments on the Major Histocompatibility Complex Class II Products of Dendritic Cells , 1998, The Journal of experimental medicine.

[40]  K. Kurosaka,et al.  Production of proinflammatory cytokines by phorbol myristate acetate-treated THP-1 cells and monocyte-derived macrophages after phagocytosis of apoptotic CTLL-2 cells. , 1998, Journal of immunology.

[41]  S. Nagata,et al.  Caspase 1-independent IL-1β release and inflammation induced by the apoptosis inducer Fas ligand , 1998, Nature Medicine.

[42]  P. Ricciardi-Castagnoli,et al.  Bystander apoptosis triggers dendritic cell maturation and antigen-presenting function. , 1998, Journal of immunology.

[43]  Loise M. Francisco,et al.  Immature Dendritic Cells Phagocytose Apoptotic Cells via αvβ5 and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes , 1998, The Journal of experimental medicine.

[44]  D. Mevorach,et al.  Systemic Exposure to Irradiated Apoptotic Cells Induces Autoantibody Production , 1998, The Journal of experimental medicine.

[45]  A. Tatum,et al.  Characteristics of auto anti-idiotypic antibodies reactive with antibodies expressing the pathogenic idiotype, IdLNF1, in the (NZB x SWR)F1 model for lupus nephritis and its parental strains. , 1998, Journal of autoimmunity.

[46]  M. Albert,et al.  Dendritic cells acquire antigen from apoptotic cells and induce class I-restricted CTLs , 1998, Nature.

[47]  S. Kalled,et al.  Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function. , 1998, Journal of immunology.

[48]  V. Fadok,et al.  Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF. , 1998, The Journal of clinical investigation.

[49]  A. Sette,et al.  Promiscuous Presentation and Recognition of Nucleosomal Autoepitopes in Lupus: Role of Autoimmune T Cell Receptor α Chain , 1998, The Journal of experimental medicine.

[50]  R. Zinkernagel,et al.  Crucial role of marginal zone macrophages and marginal zone metallophils in the clearance of lymphocytic choriomeningitis virus infection , 1997, European journal of immunology.

[51]  A. Espenes,et al.  Apoptosis in phagocytotic cells of lymphoid tissues in rainbow trout (Oncorhynchus mykiss) following administration of clodronate liposomes , 1997, Cell and Tissue Research.

[52]  N. Van Rooijen,et al.  T cell priming against vesicular stomatitis virus analyzed in situ: red pulp macrophages, but neither marginal metallophilic nor marginal zone macrophages, are required for priming CD4+ and CD8+ T cells. , 1997, Journal of immunology.

[53]  S. Muller,et al.  Specificity of monoclonal anti-nucleosome auto-antibodies derived from lupus mice. , 1996, Journal of autoimmunity.

[54]  B. Richardson,et al.  Monocyte apoptosis in patients with active lupus. , 1996, Arthritis and rheumatism.

[55]  N. Van Rooijen,et al.  Biodistribution of clodronate and liposomes used in the liposome mediated macrophage 'suicide' approach. , 1996, Journal of immunological methods.

[56]  A. Urtti,et al.  Effect of Liposomal and Free Bisphosphonates on the IL-1β, IL-6 and TNFα Secretion from RAW 264 Cells in Vitro , 1995, Pharmaceutical Research.

[57]  B. Richardson,et al.  Mechanism of drug-induced lupus. I. Cloned Th2 cells modified with DNA methylation inhibitors in vitro cause autoimmunity in vivo. , 1995, Journal of immunology.

[58]  N. Van Rooijen,et al.  Brucella abortus causes an accelerated repopulation of the spleen and liver of mice by macrophages after their liposome-mediated depletion. , 1995, Journal of medical microbiology.

[59]  J. Bach,et al.  Nucleosome-restricted antibodies are detected before anti-dsDNA and/or antihistone antibodies in serum of MRL-Mp lpr/lpr and +/+ mice, and are present in kidney eluates of lupus mice with proteinuria. , 1994, Arthritis and rheumatism.

[60]  N. Van Rooijen,et al.  Liposome mediated depletion of macrophages: mechanism of action, preparation of liposomes and applications. , 1994, Journal of immunological methods.

[61]  A. Rosen,et al.  Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes , 1994, The Journal of experimental medicine.

[62]  J. Gavalchin,et al.  Treatment with antibody reactive with the nephritogenic idiotype, IdLNF1, suppresses its production and leads to prolonged survival of (NZB x SWR)F1 mice. , 1994, Journal of autoimmunity.

[63]  E. Amento,et al.  Treating activated CD4+ T cells with either of two distinct DNA methyltransferase inhibitors, 5-azacytidine or procainamide, is sufficient to cause a lupus-like disease in syngeneic mice. , 1993, The Journal of clinical investigation.

[64]  C. Mohan,et al.  Nucleosome: a major immunogen for pathogenic autoantibody-inducing T cells of lupus , 1993, The Journal of experimental medicine.

[65]  D. Papahadjopoulos,et al.  Quantitative analysis of liposome-cell interactions in vitro: rate constants of binding and endocytosis with suspension and adherent J774 cells and human monocytes. , 1993, Biochemistry.

[66]  A. Tatum,et al.  The onset of nephritis in the (NZB x SWR)F1 murine model for systemic lupus erythematosus correlates with an increase in the ratio of CD4 to CD8 T lymphocytes specific for the nephritogenic idiotype (IdLNF1). , 1992, Clinical immunology and immunopathology.

[67]  M. Madaio,et al.  Anti-DNA antibodies form immune deposits at distinct glomerular and vascular sites. , 1992, Kidney international.

[68]  S. Hanash,et al.  Phenotypic and functional similarities between 5-azacytidine-treated T cells and a T cell subset in patients with active systemic lupus erythematosus. , 1992, Arthritis and rheumatism.

[69]  D. Papahadjopoulos,et al.  Recognition of liposomes by cells: in vitro binding and endocytosis mediated by specific lipid headgroups and surface charge density. , 1992, Biochimica et biophysica acta.

[70]  J. Pehrson Thymine dimer formation as a probe of the path of DNA in and between nucleosomes in intact chromatin. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[71]  N. Rooijen,et al.  Macrophage Subset Repopulation in the Spleen: Differential Kinetics After Liposome‐Mediated Elimination , 1989, Journal of leukocyte biology.

[72]  M. Cygler,et al.  Antibodies to DNA , 1988, BioEssays : news and reviews in molecular, cellular and developmental biology.

[73]  R. Seder,et al.  The NZB X SWR model of lupus nephritis. I. Cross-reactive idiotypes of monoclonal anti-DNA antibodies in relation to antigenic specificity, charge, and allotype. Identification of interconnected idiotype families inherited from the normal SWR and the autoimmune NZB parents. , 1987, Journal of immunology.

[74]  J. Gavalchin,et al.  The NZB X SWR model of lupus nephritis. II. Autoantibodies deposited in renal lesions show a distinctive and restricted idiotypic diversity. , 1987, Journal of immunology.

[75]  E. Gleichmann,et al.  The Lyb-3+5+ subset of B cells is not required for lupus-like autoantibody formation caused by graft-vs-host reaction. , 1985, Journal of immunology.

[76]  R. Schwartz,et al.  Lupus prone (SWR x NZB)F1 mice produce potentially nephritogenic autoantibodies inherited from the normal SWR parent. , 1985, Journal of immunology.

[77]  N. Rooijen,et al.  Elimination of phagocytic cells in the spleen after intravenous injection of liposome-encapsulated dichloromethylene diphosphonate , 1984, Cell and Tissue Research.

[78]  C. Steinman Circulating DNA in systemic lupus erythematosus. Isolation and characterization. , 1984, The Journal of clinical investigation.

[79]  G. Meuret,et al.  Kinetics of human monocytopoiesis. , 1974, Blood.

[80]  G. Meuret,et al.  Monocyte Kinetic Studies in Normal and Disease States , 1973, British journal of haematology.

[81]  G. R. Bartlett Phosphorus assay in column chromatography. , 1959, The Journal of biological chemistry.

[82]  L. Klareskog,et al.  Evidence that anti-tumor necrosis factor therapy with both etanercept and infliximab induces apoptosis in macrophages, but not lymphocytes, in rheumatoid arthritis joints: extended report. , 2005, Arthritis and rheumatism.

[83]  V. Fadok,et al.  Phosphatidylserine-dependent ingestion of apoptotic cells promotes TGF-beta1 secretion and the resolution of inflammation. , 2002, The Journal of clinical investigation.

[84]  I. Baumann,et al.  Impaired uptake of apoptotic cells into tingible body macrophages in germinal centers of patients with systemic lupus erythematosus. , 2002, Arthritis and rheumatism.

[85]  A. Manfredi,et al.  Apoptosis and systemic autoimmunity: the dendritic cell connection. , 2000, European journal of histochemistry : EJH.

[86]  R. Yung,et al.  Mechanisms of drug-induced lupus. III. Sex-specific differences in T cell homing may explain increased disease severity in female mice. , 1997, Arthritis and rheumatism.

[87]  A. Urtti,et al.  Effect of liposomal and free bisphosphonates on the IL-1 beta, IL-6 and TNF alpha secretion from RAW 264 cells in vitro. , 1995, Pharmaceutical research.

[88]  C. Kurts,et al.  Brief Definitive Report Major Histocompatibility Complex Class I–restricted Cross-presentation Is Biased towards High Dose Antigens and Those Released during Cellular Destruction , 2022 .