Supplementation of Lactic Acid Bacteria in Feed Induced Non-Specific Immune Response of Tiger Grouper (SUPLEMENTASI BAKTERI ASAM LAKTAT PADA PAKAN MERANGSANG TANGGAP KEBAL NON SPESIFIK IKAN KERAPU MACAN)

Lactic acid bacteria (LAB) have an ability to enhance innate responses in fish. The aim of this studywas to study the effect of three LAB isolates (Kerapu Situbondo Usus: KSBU 12C, KSBU 5Da, and KSBU9 on non-specific immune responses of tiger grouper (Epinephelus fuscoguttatus). The fish were fed dietsupplemented with each of the bacterial isolates at final concentration of 108 CFU mL-1 twice every day.The haematology parameters (haematocrit, leucocyte count, heterophils/neutrophils, monocytes,lymphocytes), phagocytic activity and total LAB were measured every seven days. After 28 days, the fishwere challenged with Vibrio alginolyticus. During the feeding period, the haematocrit values of fish fed withLAB isolates were higher than those of control group, and values ranged from 24.67±8.33 % to 40.50±7.09%. The leucocyte counts increased, mainly after being challenged with V. alginolyticus, and the valuesranged from 40.33 ±6.03 × 1000 cell mL-1 to 139.33±15.57 × 1000 cell mL-1. With the exception oflymphocytes, proportion of heterophils and monocytes, and phagocytic activity were higher in fish fed withLAB isolates than those of control. The presence of LAB in the gastrointestinal tract of fish fed with LABresulted in higher haematology parameters and phagocytic activity in comparison to fish fed control diet.In conclusion, supplementation of LAB in diet could enhance non-specific immune response of tiger grouper.

[1]  Kwancai A Gomez.  Prosedur statistik untuk penelitian pertanian , 2014 .

[2]  T. Triyanto,et al.  Isolasi Bakteri Asam Laktat dari Usus Ikan Kerapu Macan (Epinephelus fuscoguttatus) dan Potensinya Sebagai Antivibrio , 2012 .

[3]  M. Essa,et al.  The viability of probiotics as a factor influencing the immune response in the Nile tilapia, Oreochromis niloticus , 2011 .

[4]  M. Heo,et al.  Diet enriched with mushroom Phellinus linteus extract enhances the growth, innate immune response, and disease resistance of kelp grouper, Epinephelus bruneus against vibriosis. , 2011, Fish & shellfish immunology.

[5]  C. Chiu,et al.  Dietary administration of the probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper Epinephelus coioides. , 2009, Fish & shellfish immunology.

[6]  V. Arul,et al.  Inhibitory activity of probiotics Streptococcus phocae PI80 and Enterococcus faecium MC13 against Vibriosis in shrimp Penaeus monodon , 2009 .

[7]  M. Gershwin,et al.  Probiotics and immunity , 2009, Journal of Gastroenterology.

[8]  R. Kumar,et al.  Enhanced innate immune parameters in Labeo rohita (Ham.) following oral administration of Bacillus subtilis. , 2008, Fish & shellfish immunology.

[9]  M. Endo,et al.  Protective effects and mechanisms of a probiotic bacterium Lactobacillus rhamnosus against experimental Edwardsiella tarda infection in tilapia (Oreochromis niloticus). , 2006, Veterinary immunology and immunopathology.

[10]  A. Ambariyanto,et al.  Uji Keganasan Bakteri Vibrio pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) , 2006 .

[11]  T. Triyanto,et al.  ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO , 2005 .

[12]  Takeshi Kobayashi,et al.  The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss , 2005 .

[13]  T. Kobayashi,et al.  Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus rhamnosus JCM 1136. , 2004, Veterinary immunology and immunopathology.

[14]  B. Austin,et al.  Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum) , 2002 .

[15]  Nwadiuto Esiobu,et al.  Antibiotic resistance in soil and water environments , 2002, International journal of environmental health research.

[16]  B. Gómez‐Gil,et al.  The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms , 2000 .

[17]  B. Austin,et al.  Use of Carnobacterium sp. as a probiotic for Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss, Walbaum). , 2000 .

[18]  C. Secombes.  2 - The Nonspecific Immune System: Cellular Defenses , 1996 .

[19]  D. P. Anderson,et al.  Basic hematology and serology for fish health programs , 1995 .