Correlated effects of acetylcholine and cyclic guanosine monophosphate on membrane properties of mammalian neocortical neurons.

The effects of successive extracellular iontophoresis of acetylcholine (ACh) and atropine, and intracellular hyperpolarizing iontophoresis of cyclic GMP (cGMP) were studied in single neurons of the coronal-pericruciate cortex of awake cats. (a) Fifty-seven percent of the neurons that were tested responded to ACh with an increase in neuronal input resistance (Rm) and 50% responded to ACh with an increase in firing rate; 65% responded to cGMP with an increase in Rm and 60% responded to cGMP with an increase in firing rate. (b) After application of atropine, increases in Rm and firing rate associated with iontophoresis of ACh failed to recur. (c) Persistent increases in Rm following application of ACh accompanied by current-induced neuronal discharge were not diminished by subsequent application of atropine. (d) Atropine did not prevent increases in Rm and firing rate associated with intracellular iontophoresis of cGMP. (e) All cells tested with both ACh and cGMP that were shown initially to respond to extracellular ACh with increases in Rm were later shown to have comparable responses to cGMP.

[1]  C. D. Woody,et al.  Effects of acetylcholine and cyclic GMP on input resistance of cortical neurons in awake cats , 1978, Brain Research.

[2]  C. D. Woody,et al.  Characterization of electrophysiological properties of intracellularly recorded neurons in the neocortex of awake cats: A comparison of the response to injected current in spike overshoot and undershoot neurons , 1978, Brain Research.

[3]  P. A. Smith,et al.  Synaptic potentials in sympathetic ganglia: are they mediated by cyclic nucleotides? , 1978, Science.

[4]  TOSHIO HASHIGUCHI,et al.  Postsynaptic modulation of excitatory process in sympathetic ganglia by cyclic AMP , 1978, Nature.

[5]  B. Libet,et al.  Does cyclic GMP mediate the slow excitatory synaptic potential in sympathetic ganglia? , 1978, Nature.

[6]  J. Phillis.  The Role of Cyclic Nucleotides in the CNS , 1977, Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques.

[7]  E. Hulme,et al.  BIOCHEMICAL STUDIES ON MUSCARINIC ACETYLCHOLINE RECEPTORS , 1976, Journal of neurochemistry.

[8]  V. E. Dionne.  Characterization of drug iontophoresis with a fast microassay technique. , 1976, Biophysical journal.

[9]  K. Krnjević,et al.  Is cyclic guanosine monophosphate the internal 'second messenger' for cholinergic actions on central neurons? , 1976, Canadian journal of physiology and pharmacology.

[10]  C. Woody,et al.  Persistent Increases in Membrane Resistance of Neurons in Cat Motor Cortex. , 1976 .

[11]  B. Libet,et al.  Synaptic coupling into the production and storage of a neuronal memory trace , 1975, Nature.

[12]  P. Greengard,et al.  Neurotransmitters increase cyclic nucleotides in postganglionic neurons: immunocytochemical demonstration , 1975, Science.

[13]  F. Bloom,et al.  Cyclic AMP and cyclic GMP may mediate opposite neuronal responses in the rat cerebral cortex. , 1975, Science.

[14]  F. F. Weight,et al.  Guanosine 3',5'-Monophosphate in Sympathetic Ganglia: Increase Associated with Synaptic Transmission , 1974, Science.

[15]  J. Phillis,et al.  Depression of corticospinal cells by various purines and pyrimidines. , 1974, Canadian journal of physiology and pharmacology.

[16]  W. Zieglgänsberger,et al.  Iontophoretic release of substances from micropipettes in vitro. , 1974, Neuropharmacology.

[17]  W. Zieglgänsberger,et al.  A cholinergic mechanism in the spinal cord of cats. , 1974, Neuropharmacology.

[18]  P. Greengard,et al.  Guanosine 3':5'-cyclic monophosphate-dependent phosphorylation of endogenous substrate proteins in membranes of mammalian smooth muscle. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[19]  C D Woody,et al.  Differences in excitability of cortical neurons as a function of motor projection in conditioned cats. , 1973, Journal of neurophysiology.

[20]  P. Greengard,et al.  Adenosine 3',5'-Monophosphate: Electrophysiological Evidence for a Role in Synaptic Transmission , 1972, Science.

[21]  J. Phillis,et al.  Acetylcholine inhibition in the intact and chronically isolated cerebral cortex , 1972, British journal of pharmacology.

[22]  F. Bloom,et al.  CYCLIC AMP MEDIATION OF NOREPINEPHRINE INHIBITION IN RAT CEREBELLAR CORTEX: A UNIQUE CLASS OF SYNAPTIC RESPONSES , 1971, Annals of the New York Academy of Sciences.

[23]  K. Krnjević,et al.  The mechanism of excitation by acetylcholine in the cerebral cortex , 1971, The Journal of physiology.

[24]  B J Hoffer,et al.  Studies on norepinephrine-containing afferents to Purkinje cells of rat cerebellum. 3. Evidence for mediation of norepinephrine effects by cyclic 3',5'-adenosine monophosphate. , 1971, Brain research.

[25]  T. Narahashi,et al.  Effects of procaine on ionic conductances of end-plate membranes. , 1971, The Journal of pharmacology and experimental therapeutics.

[26]  J. Ferrendelli,et al.  The effect of oxotremorine and atropine on cGMP and cAMP levels in mouse cerebral cortex and cerebellum. , 1970, Biochemical and biophysical research communications.

[27]  W. George,et al.  Elevation of guanosine 3',5'-cyclic phosphate in rat heart after perfusion with acetylcholine. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[28]  Y Fujita,et al.  Activity of dendrites of single Purkinje cells and its relationship to so-called inactivation response in rabbit cerebellum. , 1968, Journal of neurophysiology.

[29]  J. Phillis,et al.  Cholinergic inhibition in the cerebral cortex. , 1967, Brain research.

[30]  D A Pollen,et al.  Electrical constants of neurons in the motor cortex of the cat. , 1966, Journal of neurophysiology.

[31]  K. Takahashi,et al.  Slow and fast groups of pyramidal tract cells and their respective membrane properties. , 1965, Journal of neurophysiology.

[32]  K. Krnjević,et al.  Iontophoretic studies of neurones in the mammalian cerebral cortex , 1963, The Journal of physiology.

[33]  M. Fuortes,et al.  Stimulation of spinal motoneurones with intracellular electrodes , 1956, The Journal of physiology.

[34]  F. Bloom.  The role of cyclic nucleotides in central synaptic function. , 1975, Reviews of physiology, biochemistry and pharmacology.

[35]  J. Phillis.  Evidence for Cholinergic Transmission in the Cerebral Cortex , 1974 .

[36]  P. Greengard,et al.  On the mode of action of local anesthetics. , 1966, Annual review of pharmacology.