Effects of gonadotrophin in vivo and 2-hydroxyoestradiol-17beta in vitro on follicular steroid hormone profile associated with oocyte maturation in the catfish Heteropneustes fossilis.

An HPLC method was used to tentatively identify progesterone (P4) and its metabolites (17-hydroxyprogesterone (17-P4) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P)), corticosteroids (cortisol and corticosterone) and testosterone in ovary/follicular preparations of the catfish Heteropneustes fossilis associated with in vivo or in vitro oocyte maturation/ovulation. A single i.p. injection of human chorionic gonadotrophin (100 IU/fish, sampled at 0, 8 and 16 h) induced oocyte maturation and ovulation, which coincided with significant and progressive increases in 17,20beta-P, and P4 and 17-P4, the precursors of the former. Both cortisol and corticosterone also increased significantly. Conversely, testosterone decreased significantly and progressively over time. Under in vitro conditions, incubation of post-vitellogenic (intact) follicles or follicular envelope (layer) with 2-hydroxyoestradiol (2-OHE2, 5 microM for 0, 6 and 24 h) elicited a sharp significant increase in 17,20beta-P, the increase being higher in the follicular envelope incubate. P4 and 17-P4 also registered significant increases over the time with the peak values at 24 h. Cortisol and corticosterone increased significantly in the intact follicle, but not in the follicular envelope incubate. Testosterone decreased significantly in the intact follicle, but increased significantly (24 h) in the follicular envelope incubate. Coincident with these changes, the percentage of germinal vesicle breakdown (GVBD) increased over the time in the intact follicle incubate (48.9% at 6 h and 79.8% at 24 h). Denuded oocytes on incubation with 2-OHE2 (5 microM) did not produce any significant change in the percentage of GVBD or in the steroid profile. While corticosterone and 17,20beta-P were undetected, P4, 17-P4, cortisol and testosterone were detected in low amounts. The results show that the 2-OHE2-induced GVBD response seems to be mediated through the production of 17,20beta-P and corticosteroids. It is suggested that hydroxyoestrogens seem to be a component in the gonadotrophin cascade of regulation of oocyte maturation/ovulation in the catfish.

[1]  M. Yamashita,et al.  Regulation of oocyte maturation in fish , 2008, Development, growth & differentiation.

[2]  A. Mishra,et al.  Relative effects of estradiol-17beta (E2), catecholestrogens and clomiphene citrate on in vitro oocyte maturation in the catfish Heteropneustes fossilis (Bloch) and E2 inhibition of 2-hydroxyestradiol-induced maturation. , 2006, General and comparative endocrinology.

[3]  K. P. Joy,et al.  HPLC-electrochemical detection of ovarian estradiol-17beta and catecholestrogens in the catfish Heteropneustes fossilis: seasonal and periovulatory changes. , 2006, General and comparative endocrinology.

[4]  G. Somoza,et al.  Steroid metabolism in vitro during final oocyte maturation in white croaker Micropogonias furnieri (Pisces: Sciaenidae). , 2004, Brazilian journal of biology = Revista brasleira de biologia.

[5]  P. Swanson,et al.  Biochemistry and physiology of fish gonadotropins , 2003, Fish Physiology and Biochemistry.

[6]  H. Schatten,et al.  Inhibitory effects of cAMP and protein kinase C on meiotic maturation and MAP kinase phosphorylation in porcine oocytes , 2002, Molecular reproduction and development.

[7]  C. Schreck,et al.  Ovarian steroidogenesis in white sturgeon (Acipenser transmontanus) during oocyte maturation and induced ovulation. , 2002, General and comparative endocrinology.

[8]  B. Senthilkumaran,et al.  Periovulatory changes in catfish ovarian oestradiol-17beta, oestrogen-2-hydroxylase and catechol-O-methyltransferase during GnRH analogue-induced ovulation and in vitro induction of oocyte maturation by catecholoestrogens. , 2001, The Journal of endocrinology.

[9]  B. Walker,et al.  The Journal of Clinical Endocrinology & Metabolism Printed in U.S.A. Copyright © 2000 by The Endocrine Society Development-Related Increase in Cortisol Biosynthesis , 2022 .

[10]  B. Tharakan,et al.  Induced Spawning of the Indian Catfish, Heteropneustes fossilis, by GnRH Analogue Alone or in Combination with Dopamine-Affecting Drugs , 1999 .

[11]  A. Trounson,et al.  Oocyte maturation. , 1998, Human reproduction.

[12]  Y. Nagahama.  17α,20β-Dihydroxy-4-pregnen-3-one, a maturation-inducing hormone in fish oocytes: Mechanisms of synthesis and action , 1997, Steroids.

[13]  M. Tagawa.  Current understanding of the presence of hormones in fish eggs , 1996 .

[14]  D. Armstrong,et al.  Catecholestrogen modulation of steroid production by rat luteal cells: Mechanism of action , 1994, Molecular and Cellular Endocrinology.

[15]  Minoru Tanaka,et al.  13 Regulation of Oocyte Maturation in Fish , 1994 .

[16]  M. Zerani,et al.  Prostaglandin E2 and prostaglandin F2 alpha involvement in the corticosterone and cortisol release by the female frog, Rana esculenta, during ovulation. , 1993, The Journal of experimental zoology.

[17]  D. Kime,et al.  In vitro biosynthesis of steroids, including 11-deoxycortisol and 5α-pregnane-3β,7α,17,20β-tetrol, by ovaries of the goldfish Carassius auratus during the stage of oocyte final maturation , 1992 .

[18]  P. Thomas,et al.  Evidence for a role of Na+,K(+)-ATPase in the hydration of Atlantic croaker and spotted seatrout oocytes during final maturation. , 1991, The Journal of experimental zoology.

[19]  A. Scott,et al.  Plasma levels of ovarian steroids, including 17α,21-dihydroxy-4-pregnene-3,20-dione and 3α,17α,21-trihydroxy-5β-pregnan-20-one, in female plaice (Pleuronectes platessa) induced to mature with human chorionic gonadotrophin , 1990 .

[20]  R. Wallace,et al.  Steroidogenesis in Fundulus heteroclitus: II. Production of 17α-hydroxy-20β-dihydroprogesterone, testosterone, and 17β-estradiol by various components of the ovarian follicle , 1989 .

[21]  D. Armstrong,et al.  Catecholestrogens inhibit basal and luteinizing hormone-stimulated androgen production by porcine thecal cells. , 1989, Biology of reproduction.

[22]  J. Hammond,et al.  Mechanism of action of 2-hydroxyestradiol on steroidogenesis in ovarian granulosa cells: interactions with catecholamines and gonadotropins involve cyclic adenosine monophosphate. , 1989, Biology of reproduction.

[23]  N. Suzuki,et al.  Influence of follicular development on steroid production in the medaka (Oryzias latipes) ovarian follicle in response to exogenous substrates. , 1988, General and comparative endocrinology.

[24]  J. Hammond,et al.  Comparative effects of androgens and catecholestrogens on progesterone production by porcine granulosa cells , 1988, Molecular and Cellular Endocrinology.

[25]  E. Eding,et al.  The effect of pimozide/LHRHa and 17α-hydroxyprogesterone on plasma steroid levels and ovulation in the African catfish, Clarias gariepinus , 1987 .

[26]  F. Goetz,et al.  Shift in Steroidogenesis in the Ovarian Follicles of the Goldfish (Carassius auratus) during Gonadotropin‐Induced Oocyte Maturation , 1986, Development, growth & differentiation.

[27]  R. Wallace,et al.  Oocyte maturation in the mummichog (Fundulus heteroclitus): effects of steroids on germinal vesicle breakdown of intact follicles in vitro. , 1986, General and comparative endocrinology.

[28]  P. Thomas,et al.  Identification of 17α,20β,21-trihydroxy-4-pregnen-3-one as the major ovarian steroid produced by the teleost Micropogonias undulatus during final oocyte maturation , 1986, Steroids.

[29]  P. Thomas,et al.  Identification of 17 alpha,20 beta,21-trihydroxy-4-pregnen-3-one as the major ovarian steroid produced by the teleost Micropogonias undulatus during final oocyte maturation. , 1986, Steroids.

[30]  Y. Nagahama,et al.  Identification of maturation-inducing steroid in a teleost, the amago salmon (Oncorhynchus rhodurus). , 1985, Developmental biology.

[31]  P. Talbot.  Hyaluronidase dissolves a component in the hamster zona pellucida. , 1984, The Journal of experimental zoology.

[32]  F. Goetz,et al.  The in vitro synthesis of final maturational steroids by ovaries of brook trout (Salvelinus fontinalis) and yellow perch (Perca flavescens). , 1983, General and comparative endocrinology.

[33]  F. Goetz.  3 Hormonal Control of Oocyte Final Maturation and Ovulation in Fishes , 1983 .

[34]  Y. Zohar,et al.  7 The Gonadal Steroids , 1983 .

[35]  Y. Nagahama,et al.  Estradiol-17 beta production in amago salmon (Oncorhynchus rhodurus) ovarian follicles: role of the thecal and granulosa cells. , 1982, General and comparative endocrinology.

[36]  V. Lamba,et al.  Some aspects of oocyte maturation in catfish. , 1979, Journal of steroid biochemistry.

[37]  S. V. Goswami,et al.  Hormonal Regulation of in vivo and in vitro oocyte maturation in the catfish, Heteropneustes fossils (Bloch). , 1977, General and comparative endocrinology.

[38]  S. V. Goswami,et al.  Formation of 3α-hydroxy-5β-pregnan-20-one in the ovaries of catfish, Heteropneustes fossilis (bloch) , 1977 .

[39]  H. Bern,et al.  Biosynthesis of 11-deoxycorticosteroids by teleost ovaries and discussion of their possible role in oocyte maturation and ovulation. , 1973, General and comparative endocrinology.

[40]  S. V. Goswami,et al.  In vitro maturation and ovulation of oocytes of the catfish, Heteropneustes fossilis (Bloch): effects of mammalian hypophyseal hormones, catfish pituitary homogenate, steroid precursors and metabolites, and gonadal and adrenocortical steroids. , 1971, The Journal of experimental zoology.