Comparative analysis of telomere lengths and erosion with age in human epidermis and lingual epithelium.

We investigated progressive telomere shortening in normal human epidermis and lingual epithelium during aging, and attempted, in particular, to ascertain whether the telomere shortening that accompanies aging occurs at the same rate in different tissues. We studied telomeric DNA integrity, and estimated annual telomere loss, in 52 specimens of epidermis and 48 specimens of lingual epithelium collected at autopsy from subjects who had died at ages between 0 and 101 y. Most of the DNA samples were measured twice by southern blot hybridization. In addition, the correlation between telomere lengths in the two types of tissues was examined. The telomere reduction rates in epidermis and lingual epithelium were 36 bp and 30 bp per y, respectively, and these were significantly different. The rates obtained by the second measurements in epidermis and lingual epithelium were 39 and 32 bp per y, respectively, and these were also significantly different. The mean telomere lengths in the epidermis of eight neonates and the lingual epithelium of seven neonates were 13.2+/-1.0 and 13.8+/-1.0 kb, respectively. Comparison of telomere lengths in the two tissues for 41 paired samples showed that the mean telomere length in the epidermis (10.7+/-2.3 kb) was less than that in the lingual epithelium (12.4+/-2.5 kb); however, statistical analysis revealed a very significant relationship between epidermal and lingual epithelial telomere length (r=0.842, p<0.0001). These results indicate that the telomeres in epidermis and lingual epithelium are characterized by tissue-specific loss rates.

[1]  M. Kaminishi,et al.  Telomere shortening with aging in human thyroid and parathyroid tissue , 2002, Experimental Gerontology.

[2]  M. Oshimura,et al.  Telomere lengths are characteristic in each human individual , 2002, Experimental Gerontology.

[3]  K. Mafune,et al.  Demonstration of human telomerase reverse transcriptase in human colorectal carcinomas by in situ hybridization. , 2002, International journal of oncology.

[4]  N. Samani,et al.  Telomere shortening in atherosclerosis , 2001, The Lancet.

[5]  U. Klotz,et al.  Telomere length in different tissues of elderly patients , 2000, Mechanisms of Ageing and Development.

[6]  M. Oshimura,et al.  Telomere shortening with aging in human liver. , 2000, The journals of gerontology. Series A, Biological sciences and medical sciences.

[7]  M. Oshimura,et al.  Correlation of telomere lengths in normal and cancers tissue in the large bowel. , 2000, Cancer letters.

[8]  J. Skurnick,et al.  Telomere attrition of the human abdominal aorta: relationships with age and atherosclerosis. , 2000, Atherosclerosis.

[9]  R. Hodes,et al.  Lineage-Specific Telomere Shortening and Unaltered Capacity for Telomerase Expression in Human T and B Lymphocytes with Age , 2000, The Journal of Immunology.

[10]  K. Takubo,et al.  Telomere shortening in gastric carcinoma with aging despite telomerase activation , 2000, Journal of Cancer Research and Clinical Oncology.

[11]  T. Ide,et al.  Telomere reduction in human liver tissues with age and chronic inflammation. , 2000, Experimental cell research.

[12]  A. Melk,et al.  Telomere shortening in kidneys with age. , 2000, Journal of the American Society of Nephrology : JASN.

[13]  M. Ueda,et al.  Correlation of the expression of human telomerase subunits with telomerase activity in normal skin and skin tumors , 1999, Cancer.

[14]  K. Mafune,et al.  Telomere shortening with aging in human esophageal mucosa , 1999, AGE.

[15]  H. Hamakawa,et al.  Detection of telomerase activity in oral lesions. , 2007, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[16]  H. Schwartz,et al.  Comparison of chromosome telomere integrity in multiple tissues from subjects at different ages. , 1998, Cancer genetics and cytogenetics.

[17]  D. Bono.  Olovnikov’s clock: telomeres and vascular biology , 1998 .

[18]  J. Campisi.  The role of cellular senescence in skin aging. , 1998, The journal of investigative dermatology. Symposium proceedings.

[19]  E. Blackburn,et al.  The rate of telomere sequence loss in human leukocytes varies with age. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[20]  J. Shay,et al.  In situ hybridization analysis of the expression of human telomerase RNA in normal and pathologic conditions of the skin. , 1998, The Journal of investigative dermatology.

[21]  S. Lippard,et al.  Telomere loss in cells treated with cisplatin. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[22]  J. Shay,et al.  Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals , 1998, Human Genetics.

[23]  C. Harley,et al.  Extension of life-span by introduction of telomerase into normal human cells. , 1998, Science.

[24]  S. Kannan,et al.  Telomerase activity in premalignant and malignant lesions of human oral mucosa. , 1997, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[25]  J. Shay,et al.  Telomerase activity in human intestine. , 1996, International journal of oncology.

[26]  P. Boukamp,et al.  Telomerase activity in the regenerative basal layer of the epidermis inhuman skin and in immortal and carcinoma-derived skin keratinocytes. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[27]  J. Shay,et al.  Detection of telomerase activity in malignant and nonmalignant skin conditions. , 1996, The Journal of investigative dermatology.

[28]  C. Greider,et al.  Telomere length regulation. , 1996, Annual review of biochemistry.

[29]  C. Harley,et al.  Telomere length and replicative aging in human vascular tissues. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[30]  T. Lange.  10 Telomere Dynamics and Genome Instability in Human Cancer , 1995 .

[31]  G. Saretzki,et al.  Mild hyperoxia shortens telomeres and inhibits proliferation of fibroblasts: a model for senescence? , 1995, Experimental cell research.

[32]  E. Rogaev,et al.  Telomere shortening is associated with cell division in vitro and in vivo. , 1995, Experimental cell research.

[33]  C B Harley,et al.  Specific association of human telomerase activity with immortal cells and cancer. , 1994, Science.

[34]  C B Harley,et al.  Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes. , 1993, American journal of human genetics.

[35]  C B Harley,et al.  Telomere length predicts replicative capacity of human fibroblasts. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[36]  C. Harley,et al.  The telomere hypothesis of cellular aging , 1992, Experimental Gerontology.

[37]  C B Harley,et al.  Telomere end-replication problem and cell aging. , 1992, Journal of molecular biology.

[38]  E. Blackburn,et al.  Structure and function of telomeres , 1991, Nature.

[39]  H. Cooke,et al.  In vivo loss of telomeric repeats with age in humans. , 1991, Mutation research.

[40]  Robin C. Allshire,et al.  Telomere reduction in human colorectal carcinoma and with ageing , 1990, Nature.

[41]  C. Harley,et al.  Telomeres shorten during ageing of human fibroblasts , 1990, Nature.

[42]  L. S. Cram,et al.  A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[43]  D. Schwartz,et al.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis , 1984, Cell.

[44]  Cameron Il.  Cell renewal in the organs and tissues of the nongrowing adult mouse. , 1970 .

[45]  L. Hayflick.  THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS. , 1965, Experimental cell research.

[46]  L. Hayflick,et al.  The serial cultivation of human diploid cell strains. , 1961, Experimental cell research.