An integrative process for bio-ethanol production employing SSF produced cellulase without extraction

Bio-ethanol production from pretreated biomass in a single vessel was investigated in which cellulase production via SSF by Penicillium janthinellum EMS-UV-8, hydrolysis of biomass and ethanol fermentation was carried out sequentially. In this study, feasibility of using whole fermented matter with enzyme, fungal mycelia, spores and residual substrate, for the saccharification of pretreated wheat straw or avicel and its further fermentation to ethanol was investigated. Whole fermented matter produced during cellulase production via solid-state fermentation and extracted cellulase was compared for hydrolysis efficiency toward cellulosic biomass. Ethanol fermentation by Kluveromyces marxianus MTCC 4136 was investigated for both the above cases and no significant difference was observed. We could obtain similar titers of ethanol by both methods. Employing the whole fermented matter offered advantages over using extracted enzyme by reducing enzyme extraction step and thereby reducing economic constraint.

[1]  T. K. Ghose.  Measurement of cellulase activities , 1987 .

[2]  Rajeev K Sukumaran,et al.  Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases , 2010 .

[3]  Cristiane S. Farinas,et al.  Simplification of the Biomass to Ethanol Conversion Process by Using the Whole Medium of Filamentous Fungi Cultivated Under Solid-State Fermentation , 2014, BioEnergy Research.

[4]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[5]  Carlos Ricardo Soccol,et al.  Recent advances in solid-state fermentation. , 2009 .

[6]  D. Gokhale,et al.  CELLULASES FROM PENICILLIUM JANTHINELLUM MUTANTS: SOLID-STATE PRODUCTION AND THEIR STABILITY IN IONIC LIQUIDS , 2009 .

[7]  Jichu Yang,et al.  Cellulase Production by Trichoderma koningii AS3.4262 in Solid-State Fermentation Using Lignocellulosic Waste from the Vinegar Industry , 2007 .

[8]  Q. Syed,et al.  On-site cellulase production by Trichoderma reesei 3EMS35 mutant and same vessel saccharification and fermentation of acid treated wheat straw for ethanol production , 2014, EXCLI journal.

[9]  J. Saini,et al.  Bioethanol production from wheat straw via enzymatic route employing Penicillium janthinellum cellulases. , 2014, Bioresource technology.

[10]  Ankita Sharma,et al.  Blending of cellulolytic enzyme preparations from different fungal sources for improved cellulose hydrolysis by increasing synergism , 2014 .

[11]  S. Bansal,et al.  Production of Cellulases through Solid State Fermentation Using Kinnow Pulp as a Major Substrate , 2010 .

[12]  B. Wang,et al.  Research on solid-state fermentation on rice chaff with a microbial consortium. , 2004, Colloids and surfaces. B, Biointerfaces.

[13]  Reeta Rani Singhania,et al.  Improved Cellulase Production by Trichoderma reesei RUT C30 under SSF Through Process Optimization , 2007, Applied biochemistry and biotechnology.

[14]  Enoch Y Park,et al.  One-pot bioethanol production from cellulose by co-culture of Acremonium cellulolyticus and Saccharomyces cerevisiae , 2012, Biotechnology for Biofuels.

[15]  Reeta Rani Singhania,et al.  Microbial cellulases ─ Production, applications and challenges , 2005 .