In vitro invasion of transfected human breast epithelial cells MCF10A-neoT

Tumor invasion and metastasis are responsible for the progression of malignant disease. These processes are facilitated by the upregulation of various types of proteinases. In recent years, experimental and clinical studies have been carried out using inhibitors of MMPs,1,2 plasmin and plasminogen activators,3 as well as cysteine proteinases.4,5,6,7 They inhibit either growth,7 motility8 or the invasive potential4,5 of various types of tumours. The aim of the study was to evaluate the effectiveness of synthetic and peptide proteinase inhibitors in reducing in vitro invasion of MCF10A cells, transfected with ras oncogene.

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