Integrated management of Phomopsis azadirachtae, the causal organism of die-back of neem.

Phomopsis azadirachtae is the causal organism of die-back of neem. Combinations of two systemic fungicides such as carbendazim and thiophanate-methyl with ethyl acetate extract of culture filtrate of antagonistic bacterium Bacillus subtilis were evaluated against P. azadirachtae under in vitro conditions. The parameters studied were mycelial dry weight, colony diameter, pycnidial formation and the conidial germ tube growth of the pathogen. The effect of these combinations on neem seed germination and seed-borne pathogen was also tested. The results indicated that the combinations tested were effective in inhibiting the growth of the pathogen in vitro . The combinations also inhibited the growth of P. azadirachtae from die-back infected neem seeds and had no significant negative effect on neem seed germination. These combinations could be utilized for the integrated control of die-back of neem.

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