Biochemical properties of a beta-xylosidase from Clostridium cellulolyticum

A 43-kDa beta-xylosidase from Clostridium cellulolyticum was purified to homogeneity. The enzyme releases xylose from p-nitrophenylxylose and xylodextrins with a degree of polymerization ranging between 2 and 5. The N-terminal amino acid sequence of the enzyme showed homologies with three other bacterial beta-xylosidases. By proton nuclear magnetic resonance spectroscopy, the enzyme was found to act by inverting the beta-anomeric configuration.

[1]  C. Tardif,et al.  The cellulolytic system of Clostridium cellulolyticum. , 1997, Journal of biotechnology.

[2]  F. Guerlesquin,et al.  Purification and characterization of endoglucanase C from Clostridium cellulolyticum. Catalytic comparison with endoglucanase A. , 1993, European journal of biochemistry.

[3]  A Bairoch,et al.  New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. , 1993, The Biochemical journal.

[4]  K. Sakka,et al.  Nucleotide Sequence of the Clostridium stercorarium xylA Gene Encoding a Bifunctional Protein with β-D-Xylosidase and α-L-Arabinofuranosidase Activities, and Properties of the Translated Product. , 1993, Bioscience, biotechnology, and biochemistry.

[5]  K. Sakka,et al.  Nucleotide sequence of the Clostridium stercorarium xynA gene encoding xylanase A: identification of catalytic and cellulose binding domains. , 1993, Bioscience, biotechnology, and biochemistry.

[6]  C. Gaudin,et al.  Genes and Proteins Involved in Cellulose Degradation by Mesophilic Clostridia , 1993 .

[7]  C. Forsberg,et al.  Isolation of Clostridium acetobutylicum strains and the preliminary investigation of the hemicellulolytic activities of isolate 3BYR , 1992 .

[8]  C. Gaudin,et al.  Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum. , 1992, Gene.

[9]  H. Fierobe,et al.  The catalytic domain of endoglucanase A from Clostridium cellulolyticum: effects of arginine 79 and histidine 122 mutations on catalysis , 1992, Journal of bacteriology.

[10]  B. Henrissat,et al.  Stereochemistry of the hydrolysis reaction catalyzed by endoglucanase Z from Erwinia chrysanthemi , 1992, FEBS letters.

[11]  R. González,et al.  Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase , 1991, Journal of bacteriology.

[12]  B Henrissat,et al.  A classification of glycosyl hydrolases based on amino acid sequence similarities. , 1991, The Biochemical journal.

[13]  H. Fierobe,et al.  Characterization of endoglucanase A from Clostridium cellulolyticum , 1991, Journal of bacteriology.

[14]  J. Wiegel,et al.  Subunit Composition and Glycosidic Activities of the Cellulase Complex from Clostridium thermocellum JW20 , 1990, Applied and environmental microbiology.

[15]  E. Bayer,et al.  Relationship of cellulosomal and noncellulosomal xylanases of Clostridium thermocellum to cellulose-degrading enzymes , 1990, Journal of bacteriology.

[16]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[17]  H. Adelsberger,et al.  Xylan degradation by the thermophile Clostridium stercorarium: Cloning and expression of xylanase, ß-D-xylosidase, and α-L-arabinofuranosidase genes in Escherichia coli , 1990 .

[18]  E. Hehre,et al.  Stereochemical course of hydrolysis and hydration reactions catalysed by cellobiohydrolases I and II from Trichoderma reesei , 1990, FEBS letters.

[19]  K. Sakka,et al.  Cloning and Expression in Escherichia coli of Clostridium stercorarium Strain F-9 Genes Related to Xylan Hydrolysis , 1990 .

[20]  E. Faure,et al.  Sequence analysis of the Clostridium cellulolyticum endoglucanase-A-encoding gene, celCCA. , 1989, Gene.

[21]  E. Faure,et al.  Cloning and expression of two cellulase genes of Clostridium cellulolyticum in Escherichia coli. , 1988, Gene.

[22]  C. Forsberg,et al.  Isolation and Some Properties of a β-d-Xylosidase from Clostridium acetobutylicum ATCC 824 , 1987 .

[23]  R. Datta,et al.  Fermentation of xylan by Clostridium acetobutylicum , 1986 .

[24]  Christian Gaudin,et al.  Metabolism and Solubilization of Cellulose by Clostridium cellulolyticum H10 , 1985, Applied and environmental microbiology.

[25]  J. Giallo,et al.  Metabolism of glucose and cellobiose by cellulolytic mesophilic Clostridium sp. strain H10 , 1983, Applied and environmental microbiology.

[26]  P. Reilly.  Xylanases: structure and function. , 1981, Basic life sciences.

[27]  Jürgen Schmidt,et al.  Purification and some properties of five endo-1,4-β-D-xylanases and β-D-xylosidase produced by a strain of Aspergillus niger , 1979 .

[28]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.