Impact of temperature, microwave radiation and organic loading rate on methanogenic community and biogas production during fermentation of dairy wastewater.

This study analyzed dairy wastewater fermentation in convection- and microwave-heated hybrid reactors at loadings of 1 and 2 kg COD/(m3 d) and temperatures of 35 and 55 °C. The biomass was investigated at a molecular level to determine the links between the operational parameters of anaerobic digestion and methanogenic Archaea structure. The highest production of biogas with methane content of ca. 67% was noted in the mesophilic microwave-heated reactors. The production of methane-rich biogas and the overall diversity of Archaea was determined by Methanosarcinaceae presence. The temperature and the application of microwaves were the main factors explaining the variations in the methanogen community. At 35 °C, the microwave heating stimulated the growth of highly diverse methanogen assemblages, promoting Methanosarcina barkeri presence and excluding Methanosarcina harudinacea from the biomass. A temperature increase to 55 °C lowered Methanosarcinaceae abundance and induced a replacement of Methanoculleus palmolei by Methanosarcina thermophila.

[1]  M. Zieliński,et al.  Impact of microwave radiation on nitrogen removal and quantity of nitrifiers in biofilm , 2013 .

[2]  R. Amann,et al.  Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms , 1992, Applied and environmental microbiology.

[3]  Y. Akyel,et al.  Current state and implications of research on biological effects of millimeter waves: a review of the literature. , 1998, Bioelectromagnetics.

[4]  K. Sasaki,et al.  Effect of adding carbon fiber textiles to methanogenic bioreactors used to treat an artificial garbage slurry. , 2009, Journal of bioscience and bioengineering.

[5]  S. Banik,et al.  Effect of microwave irradiated Methanosarcina barkeri DSM-804 on biomethanation. , 2006, Bioresource technology.

[6]  Claude E. Shannon,et al.  A mathematical theory of communication , 1948, MOCO.

[7]  W. Verstraete,et al.  Quantifying Community Dynamics of Nitrifiers in Functionally Stable Reactors , 2007, Applied and Environmental Microbiology.

[8]  Guangwei Yu,et al.  Influence of microwave irradiation on sludge dewaterability , 2009 .

[9]  Bernhard Wett,et al.  Adaptation of Methanogenic Communities to the Cofermentation of Cattle Excreta and Olive Mill Wastes at 37°C and 55°C , 2010, Applied and Environmental Microbiology.

[10]  S Banik,et al.  Bioeffects of microwave--a brief review. , 2003, Bioresource technology.

[11]  K. Kennedy,et al.  Enhanced disinfection and methane production from sewage sludge by microwave irradiation. , 2009 .

[12]  P. Chaiprasert,et al.  Molecular Monitoring of Microbial Population Dynamics During Operational Periods of Anaerobic Hybrid Reactor Treating Cassava Starch Wastewater , 2007, Microbial Ecology.

[13]  D. Stahl,et al.  Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens , 1994, Applied and environmental microbiology.

[14]  Tian-lei Qiu,et al.  Isolation and characterization of Methanoculleus receptaculi sp. nov. from Shengli oil field, China. , 2008, FEMS microbiology letters.

[15]  M. Nei,et al.  MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. , 2011, Molecular biology and evolution.

[16]  Suiying Huang,et al.  How Stable Is Stable? Function versus Community Composition , 1999, Applied and Environmental Microbiology.

[17]  Irini Angelidaki,et al.  Influence of Environmental Conditions on Methanogenic Compositions in Anaerobic Biogas Reactors , 2005, Applied and Environmental Microbiology.

[18]  M. Dębowski,et al.  Influence of microwave radiation on bacterial community structure in biofilm , 2007 .

[19]  M. Zieliński,et al.  Microwave radiation and reactor design influence microbial communities during methane fermentation , 2012, Journal of Industrial Microbiology & Biotechnology.

[20]  E. Stackebrandt,et al.  Methanoculleus palmolei sp. nov., an irregularly coccoid methanogen from an anaerobic digester treating wastewater of a palm oil plant in north-Sumatra, Indonesia. , 1998, International journal of systematic bacteriology.

[21]  Claude E. Shannon,et al.  The Mathematical Theory of Communication , 1950 .

[22]  Ter Braak,et al.  Canoco reference manual and CanoDraw for Windows user''s guide: software for canonical community ord , 2002 .

[23]  V. O’Flaherty,et al.  Temperature dependent (37-15°C) anaerobic digestion of a trichloroethylene-contaminated wastewater. , 2011, Bioresource technology.

[24]  Stephen H. Zinder,et al.  Physiological Ecology of Methanogens , 1993 .

[25]  M. Nei,et al.  Prospects for inferring very large phylogenies by using the neighbor-joining method. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[26]  James G. Ferry,et al.  Methanogenesis : Ecology, Physiology, Biochemistry and Genetics , 1994 .

[27]  M. Zielińska,et al.  Impact of operational parameters on bacterial community in a full-scale municipal wastewater treatment plant. , 2012, Polish journal of microbiology.

[28]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[29]  T. R. Sreekrishnan,et al.  Effect of operating temperatures on the microbial community profiles in a high cell density hybrid anaerobic bioreactor. , 2012, Bioresource technology.

[30]  C. E. SHANNON,et al.  A mathematical theory of communication , 1948, MOCO.

[31]  S. Okabe,et al.  Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester. , 2007, Water research.

[32]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[33]  S. Shin,et al.  Effect of microwave irradiation on the disintegration and acidogenesis of municipal secondary sludge. , 2009 .

[34]  M. Zieliński,et al.  Impact of microwave radiation on nitrogen removal and quantity of nitrifiers in biofilmA paper submitted to the Journal of Environmental Engineering and Science. , 2010 .

[35]  M. Kimura,et al.  DGGE method for analyzing 16S rDNA of methanogenic archaeal community in paddy field soil. , 2004, FEMS microbiology letters.

[36]  Daniel B. Oerther,et al.  Use of 16S rRNA Gene Terminal Restriction Fragment Analysis To Assess the Impact of Solids Retention Time on the Bacterial Diversity of Activated Sludge , 2005, Applied and Environmental Microbiology.

[37]  Willy Verstraete,et al.  How to get more out of molecular fingerprints: practical tools for microbial ecology. , 2008, Environmental microbiology.

[38]  David A. Stahl,et al.  Development and application of nucleic acid probes , 1991 .