Identification and classification of respiratory syncytial virus (RSV) strains by surface-enhanced Raman spectroscopy and multivariate statistical techniques

There is a critical need for a rapid and sensitive means of detecting viruses. Recent reports from our laboratory have shown that surface-enhanced Raman spectroscopy (SERS) can meet these needs. In this study, SERS was used to obtain the Raman spectra of respiratory syncytial virus (RSV) strains A/Long, B1, and A2. SERS-active substrates composed of silver nanorods were fabricated using an oblique angle vapor deposition method. The SERS spectra obtained for each virus were shown to posses a high degree of reproducibility. Based on their intrinsic SERS spectra, the four virus strains were readily detected and classified using the multivariate statistical methods principal component analysis (PCA) and hierarchical cluster analysis (HCA). The chemometric results show that PCA is able to separate the three virus strains unambiguously, whereas the HCA method was able to readily distinguish an A2 strain-related G gene mutant virus (ΔG) from the A2 strain. The results described here demonstrate that SERS, in combination with multivariate statistical methods, can be utilized as a highly sensitive and rapid viral identification and classification method.

[1]  Augustus W Fountain,et al.  Classification of Chemical and Biological Warfare Agent Simulants by Surface-Enhanced Raman Spectroscopy and Multivariate Statistical Techniques , 2006, Applied spectroscopy.

[2]  M. Klempner,et al.  Characterization of the surface enhanced raman scattering (SERS) of bacteria. , 2005, The journal of physical chemistry. B.

[3]  Randall D. Tobias,et al.  Chemometrics: A Practical Guide , 1998, Technometrics.

[4]  B. Semler,et al.  Atomic force microscopy analysis of icosahedral virus RNA. , 2005, Journal of molecular biology.

[5]  Burt V. Bronk,et al.  Silver metal induced surface enhanced Raman of bacteria , 2002 .

[6]  M. Kundi,et al.  Improved detection of respiratory syncytial virus in nasal aspirates by seminested RT‐PCR , 1997, Journal of medical virology.

[7]  Richard G. Brereton,et al.  Chemometrics: Data Analysis for the Laboratory and Chemical Plant , 2003 .

[8]  D. Lim,et al.  A rapid detection method for Vaccinia virus, the surrogate for smallpox virus , 2004, Biosensors and Bioelectronics.

[9]  R. Dasari,et al.  Single Molecule Detection Using Surface-Enhanced Raman Scattering (SERS) , 1997 .

[10]  M. O’Shea,et al.  Symptomatic respiratory syncytial virus infection in previously healthy young adults living in a crowded military environment. , 2005, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[11]  L. Anderson,et al.  Respiratory Syncytial Virus G and/or SH Protein Alters Th1 Cytokines, Natural Killer Cells, and Neutrophils Responding to Pulmonary Infection in BALB/c Mice , 1999, Journal of Virology.

[12]  M. Moskovits.  Surface‐enhanced Raman spectroscopy: a brief retrospective , 2005 .

[13]  Hongxing Xu,et al.  Spectroscopy of Single Hemoglobin Molecules by Surface Enhanced Raman Scattering , 1999 .

[14]  P. Greenfield,et al.  Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy , 1999, Journal of Virological Methods.

[15]  Tieqiao Wu,et al.  Surface-enhanced Raman spectroscopy of insect nuclear polyhedrosis virus , 2001 .

[16]  M. L. Laucks,et al.  Comparison of Psychro-Active Arctic Marine Bacteria and Common Mesophillic Bacteria Using Surface-Enhanced Raman Spectroscopy , 2005, Applied spectroscopy.

[17]  Harold G. Craighead,et al.  Virus detection using nanoelectromechanical devices , 2004 .

[18]  Royston Goodacre,et al.  Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface. , 2004, Analytical chemistry.

[19]  S. B. Chaney,et al.  Polarized surface enhanced Raman and absorbance spectra of aligned silver nanorod arrays. , 2006, The journal of physical chemistry. B.

[20]  Laurie L. Wood,et al.  New biochip technology for label-free detection of pathogens and their toxins. , 2003, Journal of microbiological methods.

[21]  P. Gemperline.  Practical Guide To Chemometrics , 2006 .

[22]  Yiping Zhao,et al.  Rapid and sensitive detection of respiratory virus molecular signatures using a silver nanorod array SERS substrate. , 2006, Nano letters.

[23]  De‐Yin Wu,et al.  Surface-Enhanced Raman Scattering: From Noble to Transition Metals and from Rough Surfaces to Ordered Nanostructures , 2002 .

[24]  Yiping Zhao,et al.  Aligned silver nanorod arrays produce high sensitivity surface-enhanced Raman spectroscopy substrates , 2005 .

[25]  Paul R. Carey,et al.  Biochemical Applications of Raman and Resonance Raman Spectroscopies , 1982 .

[26]  M. Porter,et al.  Low-level detection of viral pathogens by a surface-enhanced Raman scattering based immunoassay. , 2005, Analytical chemistry.

[27]  J. Barenfanger,et al.  Clinical and Financial Benefits of Rapid Detection of Respiratory Viruses: an Outcomes Study , 2000, Journal of Clinical Microbiology.