Laser sintering fabrication of three-dimensional tissue engineering scaffolds with a flow channel network

The fabrication of tissue engineering scaffolds for the reconstruction of highly oxygen-dependent inner organs is discussed. An additive manufacturing technology known as selective laser sintering was employed to fabricate a highly porous scaffold with an embedded flow channel network. A porogen leaching system was used to obtain high porosity. A prototype was developed using the biodegradable plastic polycaprolactone and sodium chloride as the porogen. A high porosity of 90% was successfully obtained. Micro x-ray CT observation was carried out to confirm that channels with a diameter of approximately 1 mm were generated without clogging. The amount of residual salt was 930 µg while the overall volume of the scaffold was 13 cm(3), and it was confirmed that the toxicity of the salt was negligible. The hydrophilization of the scaffold to improve cell adhesion on the scaffold is also discussed. Oxygen plasma ashing and hydrolysis with sodium hydroxide, typically employed to improve the hydrophilicity of plastic surfaces, were tested. The improvement of hydrophilicity was confirmed by an increase in water retention by the porous scaffold from 180% to 500%.

[1]  D. Wise,et al.  Effect of polymer foam morphology and density on kinetics of in vitro controlled release of isoniazid from compressed foam matrices. , 1997, Journal of biomedical materials research.

[2]  Wei Sun,et al.  Recent development on computer aided tissue engineering - a review , 2002, Comput. Methods Programs Biomed..

[3]  Toshiki Niino,et al.  Avidin-biotin binding-based cell seeding and perfusion culture of liver-derived cells in a porous scaffold with a three-dimensional interconnected flow-channel network. , 2007, Biomaterials.

[4]  J. Xue,et al.  Study on hydrophilicity of polymer surfaces improved by plasma treatment , 2006 .

[5]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[6]  E. Sachlos,et al.  Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. , 2003, European cells & materials.

[7]  Colleen L Flanagan,et al.  Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering. , 2005, Biomaterials.

[8]  Rainer Schmelzeisen,et al.  Fabrication of soft tissue engineering scaffolds by means of rapid prototyping techniques , 2002 .

[9]  Alison P McGuigan,et al.  Vascularized Organoid Engineered by Modular Assembly Enables Blood Perfusion , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Robert Langer,et al.  Preparation and characterization of poly(l-lactic acid) foams , 1994 .

[11]  J. Vacanti,et al.  Tissue Engineering and Organ Structure: A Vascularized Approach to Liver and Lung , 2008, Pediatric Research.

[12]  Manabu Mizutani,et al.  Liquid acrylate-endcapped biodegradable poly(epsilon-caprolactone-co-trimethylene carbonate). II. Computer-aided stereolithographic microarchitectural surface photoconstructs. , 2002, Journal of biomedical materials research.

[13]  A. G. Mikos.  Preparation and characterization of poly (L-lactic acid) foam , 1994 .

[14]  C K Chua,et al.  Selective laser sintering of biocompatible polymers for applications in tissue engineering. , 2005, Bio-medical materials and engineering.

[15]  Wei Sun,et al.  Computer‐aided tissue engineering: overview, scope and challenges , 2004, Biotechnology and applied biochemistry.

[16]  Margam Chandrasekaran,et al.  Rapid prototyping in tissue engineering: challenges and potential. , 2004, Trends in biotechnology.

[17]  Scott J. Hollister,et al.  Freeform fabrication of Nylon‐6 tissue engineering scaffolds , 2003 .

[18]  K. Leong,et al.  Fabrication of controlled release biodegradable foams by phase separation. , 1995, Tissue engineering.

[19]  J. Vacanti,et al.  Silicon micromachining to tissue engineer branched vascular channels for liver fabrication. , 2000, Tissue engineering.

[20]  S. Zeronian,et al.  An analysis of the moisture‐related properties of hydrolyzed polyester , 1982 .

[21]  Yasuyuki Sakai,et al.  A novel poly-L-lactic acid scaffold that possesses a macroporous structure and a branching/joining three-dimensional flow channel network : its fabrication and application to perfusion culture of human hepatoma Hep G2 cells , 2004 .

[22]  Robby Ebert,et al.  Industrial freeform generation of microtools by laser micro sintering , 2005 .

[23]  Kevin E. Healy,et al.  A novel method to fabricate bioabsorbable scaffolds , 1995 .

[24]  Y. Sakai,et al.  Ultraviolet-irradiation-based photofabrication that simultaneously produces a macroporous structure and flow channels using a photoreactive biodegradable polymer and a gas-forming azoamide compound , 2004 .

[25]  J M Powers,et al.  Fabrication of biodegradable polymer scaffolds to engineer trabecular bone. , 1995, Journal of biomaterials science. Polymer edition.

[26]  Robert Langer,et al.  Biodegradable Polymer Scaffolds for Tissue Engineering , 1994, Bio/Technology.

[27]  R Langer,et al.  Novel approach to fabricate porous sponges of poly(D,L-lactic-co-glycolic acid) without the use of organic solvents. , 1996, Biomaterials.

[28]  L G Griffith,et al.  Survival and function of hepatocytes on a novel three-dimensional synthetic biodegradable polymer scaffold with an intrinsic network of channels. , 1998, Annals of surgery.