Histiocytic lymphoma with sclerosis arising from a nodular lymphoma with a special stromal reaction. An ultrastructural study

Histiocytic lymphoma with sclerosis was found together with a small nodular lymphoma in a cervical lymph node biopsy from a 48‐year‐old man. Since the cellular origin of this entity is unclear and its ultrastructure has not been described before, we processed formalin‐fixed samples for electron microscopy, which revealed three cell types: small lymphocytes with cleaved nuclei, large lymphocytes with vesticular noncleaved nuclei, and mesenchymal stromal cells occasionally bearing desmosomes or hemidesmosomes. In addition, there was an increase of the intercellular connective tissue, which consisted of a mixture of normal and fibrous long spacing collagen fibers. The results indicate that histiocytic lymphoma with sclerosis can evolve from a nodular lymphoma, and that diagnostic information can be obtained by electron microscopy of tissue samples previously fixed in formalin.

[1]  H. Rappaport,et al.  Tumors of the hematopoietic system , 1966 .

[2]  R. Dorfman,et al.  Nodlar lymphoma: An ultrastructural study of its relationship to germinal genters and a correlation of light and electron microscopic findings , 1975 .

[3]  R. Kempson,et al.  The contribution of electron microscopy to the differential diagnosis of tumors. , 1976, Beitrage zur Pathologie.

[4]  E. Jaffe,et al.  Malignant lymphomas: current classification and new observations. , 1975, Pathology annual.

[5]  K. Lennert,et al.  Electron Microscopic Atlas of Lymph Node Cytology and Pathology , 1969 .

[6]  G. Monga,et al.  Banded structures in the connective tissue of lymphomas, lymphadenitis, and thymomas , 1971, Virchows Archiv. B, Cell pathology.

[7]  H. Ioachim.  The stromal reaction of tumors: an expression of immune surveillance. , 1976, Journal of the National Cancer Institute.

[8]  H. White,et al.  Extracellular cross-striated banded structures in human connective tissue. , 1975, Tissue & cell.

[9]  I. Katayama,et al.  The value of electron microscopy in the diagnosis of Burkitt's lymphoma. , 1974, American journal of clinical pathology.

[10]  A. Montanaro,et al.  Primary splenic malignant lymphoma, histiocytic type, with sclerosis. Report of a case with long‐term survival , 1976, Cancer.

[11]  H. Rappaport,et al.  Malignant lymphoma, histiocytic type with sclerosis (sclerosing reticulum cell sarcoma) , 1972, Cancer.

[12]  J. Stockman,et al.  Immunologic and cytochemical cell markers in non-Hodgkin's lymphomas. , 1976, Laboratory investigation; a journal of technical methods and pathology.