Enhanced radiation therapy with multilayer microdisks containing radiosensitizing gold nanoparticles.

A challenge of X-ray radiation therapy is that high dose X-rays at therapeutic conditions damage normal cells. This paper describes the use of gold nanoparticle-loaded multilayer microdisks to enhance X-ray radiation therapy, where each microdisk contains over 10(5) radiosensitizing nanoparticles. The microdisks are attached on cell membranes through electrostatic interaction. Upon X-ray irradiation, more photoelectrons and Auger electrons are generated in the vicinity of the nanoparticles, which cause water ionization and lead to the formation of free radicals that damage the DNA of adjacent cancer cells. By attaching a large amount of gold nanoparticles on cancer cells, the total X-ray dose required for DNA damage and cell killing can be reduced. Due to their controllable structure and composition, multilayer microdisks can be a viable choice for enhanced radiation therapy with nanoparticles.

[1]  W. B. Warlick,et al.  Image-guided radiation therapy: techniques and strategies , 2008 .

[2]  Liyuan Ma,et al.  Single cell DNA damage/repair assay using HaloChip. , 2012, Analytical chemistry.

[3]  N. Shikazono,et al.  Nucleobase lesions and strand breaks in dry DNA thin film selectively induced by monochromatic soft X-rays. , 2009, The journal of physical chemistry. B.

[4]  Sang Hyun Cho,et al.  Estimation of tumour dose enhancement due to gold nanoparticles during typical radiation treatments: a preliminary Monte Carlo study , 2005, Physics in medicine and biology.

[5]  R. T. Ten Haken,et al.  Advances in radiation oncology , 1997, The Lancet.

[6]  R. Berndt,et al.  ELECTRON CONFINEMENT TO NANOSCALE AG ISLANDS ON AG(111) : A QUANTITATIVE STUDY , 1998 .

[7]  Liyuan Ma,et al.  Enhanced radiation therapy with internalized polyelectrolyte modified nanoparticles. , 2014, Nanoscale.

[8]  S. Hosseinimehr.  Trends in the development of radioprotective agents. , 2007, Drug discovery today.

[9]  Junfei Xia,et al.  Gold nanoparticle-packed microdisks for multiplex Raman labelling of cells. , 2014, Nanoscale.

[10]  S. Batra,et al.  Cell-penetrating peptides and antibodies: a new direction for optimizing radioimmunotherapy , 2007, European Journal of Nuclear Medicine and Molecular Imaging.

[11]  E. B. Butler,et al.  Intensity modulated radiation therapy (IMRT): a new promising technology in radiation oncology. , 1999, The oncologist.

[12]  T. Tullius,et al.  DNA strand breaking by the hydroxyl radical is governed by the accessible surface areas of the hydrogen atoms of the DNA backbone. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[13]  N. Carter,et al.  A DNA damage checkpoint response in telomere-initiated senescence , 2003, Nature.

[14]  D. Jaffray,et al.  Review of image-guided radiation therapy , 2007, Expert review of anticancer therapy.

[15]  Nicholas J. Turro,et al.  A New Method To Determine the Generation of Hydroxyl Radicals in Illuminated TiO2 Suspensions , 1997 .

[16]  N. Turro,et al.  A New Method To Determine the Generation of Hydroxyl Radicals in Illuminated TiO 2 Suspensions , 1997 .

[17]  Mainul Hossain,et al.  Nanoparticle location and material dependent dose enhancement in X-ray radiation therapy. , 2012, The journal of physical chemistry. C, Nanomaterials and interfaces.

[18]  L. Milas,et al.  Radioprotectors in tumor radiotherapy: factors and settings determining therapeutic ratio. , 1988, Pharmacology & therapeutics.

[19]  Moshi Geso,et al.  Enhancement of radiation effects by gold nanoparticles for superficial radiation therapy. , 2009, Nanomedicine : nanotechnology, biology, and medicine.

[20]  T. Lawrence.  Radiation sensitizers and targeted therapies. , 2003, Oncology.

[21]  Glenn R. Dickson,et al.  Biological consequences of nanoscale energy deposition near irradiated heavy atom nanoparticles , 2011, Scientific reports.

[22]  Nikolai G Khlebtsov,et al.  Uptake of engineered gold nanoparticles into mammalian cells. , 2014, Chemical reviews.

[23]  J. Hainfeld,et al.  The use of gold nanoparticles to enhance radiotherapy in mice. , 2004, Physics in medicine and biology.

[24]  Peipei Zhang,et al.  Microcontact printing of polyelectrolytes on PEG using an unmodified PDMS stamp for micropatterning nanoparticles, DNA, proteins and cells , 2012 .

[25]  M. Fenech,et al.  The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. , 2008, Mutation research.

[26]  Omid C. Farokhzad,et al.  Nanoparticle-Aptamer Bioconjugates , 2004, Cancer Research.

[27]  M. Comporti.  Three models of free radical-induced cell injury. , 1989, Chemico-biological interactions.

[28]  J. Hainfeld,et al.  Radiotherapy enhancement with gold nanoparticles , 2008, The Journal of pharmacy and pharmacology.

[29]  R. Langer,et al.  Synthesis and characterization of dextran-peptide-methotrexate conjugates for tumor targeting via mediation by matrix metalloproteinase II and matrix metalloproteinase IX. , 2004, Bioconjugate chemistry.

[30]  P. Wardman,et al.  Chemical radiosensitizers for use in radiotherapy. , 2007, Clinical oncology (Royal College of Radiologists (Great Britain)).

[31]  Mark A. Atwater,et al.  Extinction coefficient of gold nanoparticles with different sizes and different capping ligands. , 2007, Colloids and surfaces. B, Biointerfaces.

[32]  W. Heston,et al.  Tumor target prostate specific membrane antigen (PSMA) and its regulation in prostate cancer , 2004, Journal of cellular biochemistry.

[33]  Alan R Hounsell,et al.  Cell-specific radiosensitization by gold nanoparticles at megavoltage radiation energies. , 2011, International journal of radiation oncology, biology, physics.

[34]  Warren C W Chan,et al.  Effect of gold nanoparticle aggregation on cell uptake and toxicity. , 2011, ACS nano.

[35]  Jinmin Zhao,et al.  In vitro ovarian cancer model based on three-dimensional agarose hydrogel , 2014, Journal of tissue engineering.

[36]  Y. J. Chen,et al.  Enhanced x-ray irradiation-induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification , 2008, Nanotechnology.

[37]  Peipei Zhang,et al.  Fabrication of multilayered microparticles by integrating layer-by-layer assembly and microcontact printing. , 2011, Small.

[38]  Peipei Zhang,et al.  The Institute of Chemistry of Great Britain and Ireland. Journal and Proceedings. 1932. Part II , 1932 .