Tregs in the immune response of BALB/c mice experimentally infected with species of the Sporothrix genus.

Background: Sporotrichosis occurs through contact with contaminated soil and plant. However, the incidence of sporotrichosis as a zoonotic epidemic has increased, particularly in Rio de Janeiro. Aim: In this work, we decided to evaluate some T-cell phenotypes involved in the immune response. Materials & methods: We used flow cytometry to quantify TCD4+ and TCD8+ and Treg from immunocompetent and immunosuppressed mice infected with Sporothrix species with different levels of virulence and pathogenicity. Results: It was demonstrated the predominance of TCD4+ over the TCD8+ cells in both groups, inoculated with all the species, and percentages of Treg observed in infected immunocompetent mice. Conclusion: This regulatory phenotype can be associated with a protective immunity in the initial periods of infection.

[1]  P. Ohashi,et al.  Turning the Tide Against Regulatory T Cells , 2019, Front. Oncol..

[2]  A. Batista-Duharte,et al.  Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice. , 2018, Fungal biology.

[3]  P. Hodgkin,et al.  Regulatory T Cells Suppress Effector T Cell Proliferation by Limiting Division Destiny , 2018, Front. Immunol..

[4]  J. M. Alvarez,et al.  Immunity and Treatment of Sporotrichosis. , 2018 .

[5]  F. Conceição-Silva,et al.  Immunopathogenesis of Human Sporotrichosis: What We Already Know , 2018, Journal of fungi.

[6]  R. Zancopé-Oliveira,et al.  Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area , 2018, Memorias do Instituto Oswaldo Cruz.

[7]  R. Menezes,et al.  Experimental Hyalohyphomycosis by Purpureocillium lilacinum: Outcome of the Infection in C57BL/6 Murine Models , 2017, Front. Microbiol..

[8]  I. D. Gremião,et al.  Zoonotic Epidemic of Sporotrichosis: Cat to Human Transmission , 2017, PLoS pathogens.

[9]  M. Wingfield,et al.  The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship , 2016, Studies in mycology.

[10]  Dmitri I. Kotov,et al.  Regulatory T Cell Induction and Retention in the Lungs Drives Suppression of Detrimental Type 2 Th Cells During Pulmonary Cryptococcal Infection , 2016, The Journal of Immunology.

[11]  A. M. Rodrigues,et al.  Sporothrix chilensis sp. nov. (Ascomycota: Ophiostomatales), a soil-borne agent of human sporotrichosis with mild-pathogenic potential to mammals. , 2016, Fungal biology.

[12]  T. Hohl.  Overview of vertebrate animal models of fungal infection. , 2014, Journal of immunological methods.

[13]  G. Meintjes,et al.  A neglected epidemic: fungal infections in HIV/AIDS. , 2014, Trends in microbiology.

[14]  H. Waldmann,et al.  Expansion of Foxp3+ T-cell populations by Candida albicans enhances both Th17-cell responses and fungal dissemination after intravenous challenge , 2014, European journal of immunology.

[15]  A. Scheffold,et al.  Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi , 2013, Mucosal Immunology.

[16]  F. V. Loures,et al.  Anti-CD25 Treatment Depletes Treg Cells and Decreases Disease Severity in Susceptible and Resistant Mice Infected with Paracoccidioides brasiliensis , 2012, PloS one.

[17]  Andréia Patrícia Gomes,et al.  Linfócitos T CD4+CD25+ e a regulação do sistema imunológico: perspectivas para o entendimento fisiopatológico da sepse , 2012 .

[18]  J. Guarro,et al.  Virulence of Sporothrix luriei in a Murine Model of Disseminated Infection , 2012, Mycopathologia.

[19]  M. Wüthrich,et al.  Protective antifungal memory CD8(+) T cells are maintained in the absence of CD4(+) T cell help and cognate antigen in mice. , 2012, The Journal of clinical investigation.

[20]  R. Zancopé-Oliveira,et al.  Sporotrichosis in HIV-infected patients: report of 21 cases of endemic sporotrichosis in Rio de Janeiro, Brazil. , 2012, Medical mycology.

[21]  N. Lima,et al.  Sporotrichosis Caused by Sporothrix mexicana, Portugal , 2011, Emerging infectious diseases.

[22]  Michael J. Bevan,et al.  CD8+ T Cells: Foot Soldiers of the Immune System , 2011, Immunity.

[23]  R. Zancopé-Oliveira,et al.  Phenotypic and Molecular Identification of Sporothrix Isolates from an Epidemic Area of Sporotrichosis in Brazil , 2011, Mycopathologia.

[24]  G. Criseo,et al.  New Insight into Molecular Phylogeny and Epidemiology of Sporothrix schenckii Species Complex Based on Calmodulin-Encoding Gene Analysis of Italian Isolates , 2011, Mycopathologia.

[25]  M. Netea,et al.  T-cell Subsets and Antifungal Host Defenses , 2010, Current fungal infection reports.

[26]  D. Hafler,et al.  FOXP3+ regulatory T cells in the human immune system , 2010, Nature Reviews Immunology.

[27]  R. Zancopé-Oliveira,et al.  Sporotrichosis Caused By Sporothrix globosa in Rio De Janeiro, Brazil: Case Report , 2010, Mycopathologia.

[28]  J. Guarro,et al.  Different virulence levels of the species of Sporothrix in a murine model. , 2009, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[29]  J. Guarro,et al.  Sporothrix luriei: a rare fungus from clinical origin. , 2008, Medical mycology.

[30]  T. Miller,et al.  Changes in mouse circulating leukocyte numbers in C57BL/6 mice immunosuppressed with dexamethasone for Cryptosporidium parvum oocyst production. , 2007, Veterinary parasitology.

[31]  P. Puccetti,et al.  Immunity and Tolerance to Aspergillus Involve Functionally Distinct Regulatory T Cells and Tryptophan Catabolism1 , 2006, The Journal of Immunology.

[32]  R. Gold,et al.  New Glucocorticoids on the Horizon: Repress, Don't Activate! , 2005, The Journal of rheumatology.

[33]  S. Shoham,et al.  The immune response to fungal infections , 2005, British journal of haematology.

[34]  A. B. Kay,et al.  Lymphocytes , 1991 .

[35]  J. Convit,et al.  Studies on plating efficiency and estimation of viability of suspensions of Paracoccidioides brasiliensis yeast cells , 1980, Mycopathologia.

[36]  A. Chakrabarti,et al.  Global epidemiology of sporotrichosis. , 2015, Medical mycology.

[37]  L. Romani.  Immunity to fungal infections , 2011, Nature Reviews Immunology.