Survey of Listeria monocytogenes in ready-to-eat products: Prevalence by brands and retail establishments for exposure assessment of listeriosis in Northern Spain

The prevalence of Listeria monocytogenes in ready-to-eat products of markets in Northern Spain was studied, being analyzed 783 samples of deli meat products, smoked fish and pâte. RTE smoked fish was the most frequently contaminated food category (25% positive), with high occurrence in some brands (60% of lots positive). Significant differences in prevalence were found in in-store-packaged deli meat products (8.5%) with respect to manufacturer vacuum-packaged presentation (2.7%). Serological and molecular characterization of L. monocytogenes isolates confirmed the persistence of several clusters within manufacturing environments, as the same pulsotype was repeatedly isolated from different lots of the same brand and from different sliced products of the same store. These results reflect the need to improve hygiene and disinfection programs by addressing more accurate cleaning practices and continuous education of food workers.

[1]  J Wilson,et al.  The incidence and level of Listeria monocytogenes contamination of food sources at primary production and initial processing. , 1996, The Journal of applied bacteriology.

[2]  A. Sjöberg,et al.  Persistent and nonpersistent Listeria monocytogenes contamination in meat and poultry processing plants. , 2003, Journal of food protection.

[3]  E. H. Marth,et al.  Characteristics of Listeria monocytogenes Important to Food Processors , 2007 .

[4]  J Debevere,et al.  Incidence of Listeria monocytogenes in different types of meat products on the Belgian retail market. , 1999, International journal of food microbiology.

[5]  J. Holah,et al.  The microbial ecology of high‐risk, chilled food factories; evidence for persistent Listeria spp. and Escherichia coli strains , 2004, Journal of applied microbiology.

[6]  P. Aureli,et al.  Incidence of Listeria monocytogenes in food and environmental samples in Italy between 1990 and 1999: Serotype distribution in food, environmental and clinical samples , 2002, European Journal of Epidemiology.

[7]  D H Persing,et al.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing , 1995, Journal of clinical microbiology.

[8]  S. Loncarevic,et al.  Prevalence of Listeria monocytogenes and other Listeria spp. in Smoked and “Gravad” Fish , 1996, Acta Veterinaria Scandinavica.

[9]  K. Morgan,et al.  Food safety knowledge and practice among elderly people living at home. , 1998, Journal of epidemiology and community health.

[10]  H. Huss,et al.  Prevalence and growth of Listeria monocytogenes in naturally contaminated seafood. , 1998, International journal of food microbiology.

[11]  A. Standaert,et al.  Effect of environmental parameters (temperature, pH and a(w)) on the individual cell lag phase and generation time of Listeria monocytogenes. , 2006, International journal of food microbiology.

[12]  A. Vitas,et al.  Occurrence of Listeria monocytogenes in fresh and processed foods in Navarra (Spain). , 2004, International journal of food microbiology.

[13]  Alexandra Lianou,et al.  A review of the incidence and transmission of Listeria monocytogenes in ready-to-eat products in retail and food service environments. , 2007, Journal of food protection.

[14]  J. Zumalacárregui,et al.  Prevalence and contamination levels of Listeria monocytogenes in smoked fish and pâté sold in Spain. , 2001, Journal of food protection.

[15]  Martin Wiedmann,et al.  Molecular Subtyping to Detect Human Listeriosis Clusters , 2003, Emerging infectious diseases.

[16]  R. Elson,et al.  Microbiological examination of ready‐to‐eat cold sliced meats and pâté from catering and retail premises in the UK , 2004, Journal of applied microbiology.

[17]  C. Hedberg,et al.  Increasing Incidence of Listeriosis in France and Other European Countries , 2008, Emerging infectious diseases.

[18]  L. Rantala,et al.  Occurrence and typing of Listeria monocytogenes strains in retail vacuum-packed fish products and in a production plant. , 1999, International journal of food microbiology.

[19]  R. Lindqvist,et al.  Home storage temperatures and consumer handling of refrigerated foods in Sweden. , 2004, Journal of food protection.

[20]  E. H. Marth,et al.  Listeria, Listeriosis, and Food Safety, Third Edition , 2007 .

[21]  Chinling Wang,et al.  Genetic relatedness between Listeria monocytogenes isolates from seafood and humans using PFGE and REP-PCR. , 2006, International journal of food microbiology.

[22]  J. Hudson,et al.  Microbiological survey of prepackaged pâté and ham in New Zealand , 2005, Letters in applied microbiology.

[23]  V. Scott,et al.  Survey of Listeria monocytogenes in ready-to-eat foods. , 2003, Journal of food protection.

[24]  G. Wirtanen,et al.  Ecology of Listeria spp. in a fish farm and molecular typing of Listeria monocytogenes from fish farming and processing companies. , 2006, International journal of food microbiology.

[25]  Katherine M. Kosa,et al.  Consumer home refrigeration practices: results of a web-based survey. , 2007, Journal of food protection.

[26]  W. Goebel,et al.  Listeria Pathogenesis and Molecular Virulence Determinants , 2001, Clinical Microbiology Reviews.

[27]  P W Andrew,et al.  Listeria monocytogenes adheres to many materials found in food‐processing environments , 2001, Journal of applied microbiology.

[28]  G. Salvat,et al.  Prevalence and growth of Listeria monocytogenes in naturally contaminated cold‐smoked salmon , 2007, Letters in applied microbiology.

[29]  Hjörleifur Einarsson,et al.  Tracing Listeria monocytogenes isolates from cold-smoked salmon and its processing environment in Iceland using pulsed-field gel electrophoresis. , 2005, International journal of food microbiology.

[30]  B. Swaminathan,et al.  PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis. , 2001, International journal of food microbiology.

[31]  Washington,et al.  Considerations for establishing safety-based consume-by date labels for refrigerated ready-to-eat foods. , 2005 .

[32]  H. Seeliger,et al.  Chapter II Serotyping of Listeria monocytogenes and Related Species , 1979 .