Fermentative hydrogen gas production using biosolids pellets as the inoculum source.

Biosolids pellets produced from anaerobically digested municipal wastewater sludge by drying to greater than 90% total solids at 110-115 degrees C for at least 75 min, were tested for their suitability as an inoculum source for fermentative hydrogen production. The hydrogen recoveries (mg gaseous H(2) produced as COD/mg added substrate COD) for glucose-fed batch systems were equal, 20.2-21.5%, between biosolids pellets and boiled anaerobic digester sludge as inoculum sources. Hydrogen recoveries from primary sludge were 2.4% and 3.5% using biosolids pellets and boiled sludge, respectively, and only 0.2% and 0.8% for municipal wastewater. Biosolids pellets should be a practical inoculum source for fermentative hydrogen reactors, although the effectiveness will depend on the wastewater treated.

[1]  Han-Qing Yu,et al.  Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic cultures , 2002 .

[2]  J. Lay,et al.  Feasibility of biological hydrogen production from organic fraction of municipal solid waste , 1999 .

[3]  D. L. Hawkes,et al.  Sustainable fermentative hydrogen production: challenges for process optimisation , 2002 .

[4]  A. Breure,et al.  Role of anaerobic spore-forming bacteria in the acidogenesis of glucose: Changes induced by discontinuous or low-rate feed supply , 2006, Antonie van Leeuwenhoek.

[5]  Peter Hoffmann,et al.  Tomorrow's Energy: Hydrogen, Fuel Cells, and the Prospects for a Cleaner Planet , 2001 .

[6]  D. L. Hawkes,et al.  Enhancement of hydrogen production from glucose by nitrogen gas sparging. , 2000 .

[7]  Y. Ueno,et al.  Hydrogen Production from Industrial Wastewater by Anaerobic Microflora in Chemostat Culture , 1996 .

[8]  Samir Kumar Khanal,et al.  Biohydrogen Production in Continuous‐Flow Reactor Using Mixed Microbial Culture , 2006, Water environment research : a research publication of the Water Environment Federation.

[9]  Sang-Eun Oh,et al.  Biohydrogen gas production from food processing and domestic wastewaters , 2005 .

[10]  J. Horiuchi,et al.  Selective production of organic acids in anaerobic acid reactor by pH control. , 2002, Bioresource technology.

[11]  H. Yokoi,et al.  Microbial production of hydrogen from starch-manufacturing wastes , 2002 .

[12]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[13]  Sang-Eun Oh,et al.  Biological hydrogen production measured in batch anaerobic respirometers. , 2002, Environmental science & technology.

[14]  Jeremy T Kraemer,et al.  Continuous fermentative hydrogen production using a two-phase reactor system with recycle. , 2005, Environmental science & technology.

[15]  B. Chang,et al.  Producing hydrogen from wastewater sludge by Clostridium bifermentans. , 2003, Journal of biotechnology.

[16]  Hong Liu,et al.  Effect of pH on hydrogen production from glucose by a mixed culture. , 2002, Bioresource technology.

[17]  Heguang Zhu,et al.  Evaluation of alternative methods of preparing hydrogen producing seeds from digested wastewater sludge , 2006 .

[18]  D. Bagley,et al.  Fermentative hydrogen production in a system using anaerobic digester sludge without heat treatment as a biomass source. , 2005, Water science and technology : a journal of the International Association on Water Pollution Research.

[19]  C-C. Chen,et al.  Kinetics of hydrogen production with continuous anaerobic cultures utilizing sucrose as the limiting substrate , 2001, Applied Microbiology and Biotechnology.

[20]  T. Noike,et al.  Inhibition of hydrogen fermentation of organic wastes by lactic acid bacteria , 2002 .

[21]  J. Tay,et al.  Production of hydrogen and methane from wastewater sludge using anaerobic fermentation. , 2004, Water science and technology : a journal of the International Association on Water Pollution Research.

[22]  J. Lay,et al.  Modeling and optimization of anaerobic digested sludge converting starch to hydrogen , 2000, Biotechnology and bioengineering.

[23]  Chin-Chao Chen,et al.  Acid–base enrichment enhances anaerobic hydrogen production process , 2001, Applied Microbiology and Biotechnology.