论文信息 - External RNA Controls Consortium Beta Version Update

External RNA Controls Consortium Beta Version Update

Spike-in RNAs are valuable controls for a variety of gene expression measurements. The External RNA Controls Consortium developed test sets that were used in a number of published reports. Here we provide an authoritative table that summarizes, updates, and corrects errors in the test version that ultimately resulted in the certified Standard Reference Material 2374. We have noted existence of anti-sense RNA controls in the material, corrected sub-pool memberships, and commented on control RNAs that displayed inconsistent behavior.

[1] Leming Shi,et al. mRNA enrichment protocols determine the quantification characteristics of external RNA spike-in controls in RNA-Seq studies , 2013, Science China Life Sciences.

[2] B. Oliver,et al. Microarrays, deep sequencing and the true measure of the transcriptome , 2011, BMC Biology.

[3] Marc Salit,et al. Evaluation of the External RNA Controls Consortium (ERCC) reference material using a modified Latin square design , 2016, bioRxiv.

[4] Kathleen F. Kerr,et al. The External RNA Controls Consortium: a progress report , 2005, Nature Methods.

[5] L. Reid,et al. Proposed methods for testing and selecting the ERCC external RNA controls , 2005, BMC Genomics.

[6] M. Salit,et al. Synthetic Spike-in Standards for Rna-seq Experiments Material Supplemental Open Access License Commons Creative , 2022 .

[7] David P. Kreil,et al. Assessing technical performance in differential gene expression experiments with external spike-in RNA control ratio mixtures , 2014, Nature Communications.