Comparative studies on tetrachloroethene reductive dechlorination mediated by Desulfitobacterium sp. strain PCE-S

Tetrachloroethene reductive dechlorination was studied with cell extracts of a newly isolated, tetrachloroethene-utilizing bacterium, Desulfitobacterium sp. strain PCE-S. Tetrachloroethene dehalogenase mediated the reductive dechlorination of tetrachloroethene and trichloroethene to cis-1,2-dichloroethene with artificial electron donors such as methyl viologen. The chlorinated aromatic compounds tested so far were not reduced. A low-potential electron donor (E0′ < –0.4 V) was required for tetrachloroethene reduction. The enzyme in its reduced state was inactivated by propyl iodide and reactivated by light, indicating the involvement of a corrinoid in reductive tetrachloroethene dechlorination.

[1]  J. Suflita,et al.  Characterization of Chloroethylene Dehalogenation by Cell Extracts of Desulfomonile tiedjei and Its Relationship to Chlorobenzoate Dehalogenation , 1996, Applied and environmental microbiology.

[2]  G. Diekert,et al.  Anaerobic dehalogenases. , 1997, Current opinion in biotechnology.

[3]  P. Sharma,et al.  Isolation and Characterization of a Facultatively Aerobic Bacterium That Reductively Dehalogenates Tetrachloroethene to cis-1,2-Dichloroethene , 1996, Applied and environmental microbiology.

[4]  L. Krumholz,et al.  A freshwater anaerobe coupling acetate oxidation to tetrachloroethylene dehalogenation , 1996, Applied and environmental microbiology.

[5]  A. Zehnder,et al.  Titanium (III) citrate as a nontoxic oxidation-reduction buffering system for the culture of obligate anaerobes. , 1976, Science.

[6]  B. Fathepure,et al.  Anaerobic Bacteria ThatDechlorinate Perchloroethenet , 1987 .

[7]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[8]  P. Lawson,et al.  Desulfitobacterium sp. strain PCE1, an anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene or ortho-chlorinated phenols , 1996, Archives of Microbiology.

[9]  C. Holliger,et al.  The proton/electron ration of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus" , 1996, Journal of bacteriology.

[10]  A. Neumann,et al.  Purification and Characterization of Tetrachloroethene Reductive Dehalogenase from Dehalospirillum multivorans* , 1996, The Journal of Biological Chemistry.

[11]  Desulfomonile tiedjei.  Tetrachloroethene and 3-chlorobenzoate dechlorination activities are co-induced in Desulfomonile tiedjei DCB-1 , 2022 .

[12]  S. Ni,et al.  Purification and characterization of a novel 3-chlorobenzoate-reductive dehalogenase from the cytoplasmic membrane of Desulfomonile tiedjei DCB-1 , 1995, Journal of bacteriology.