ASSESSMENT OF RESPIRATORY HYPERSENSITIVITY IN GUINEA PIGS SENSITIZED TO TOLUENE DIISOCYANATE: A COMPARISON OF SENSITIZATION PROTOCOLS

This study used a combined intradermal injection/repeated inhalation sensitization protocol in the guinea pig with subsequent inhalation challenge with toluene diisocyanate (TDI) and the homologous TDI-protein conjugate, immunoglobulin G (IgG)-antibody 1 1 analysis, and histopathological examination of the lung as a method to evaluate the potential of TDI to induce respiratory allergy in this animal model. In order to distinguish specific from nonspecific respiratory response, guinea pigs were subjected to an additional acetylcholine (ACh) bronchoprovocation assay, 1 day before and after the challenge with TDI. For comparison, groups of guinea pigs were sensitized to TDI by combined single intradermal injection (0.3% TDI in 100 mul corn oil) and repeated inhalation exposure (3 h/day for 5 consecutive days) to 0, 3.8, 11, 26, 46, and 51 mg TDI/m 3 air. One group of animals was sensitized by intradermal injection only. Naive and polyisocyanate resin (50 and 250 mg dust/m 3 air) sensitized animals served as ...

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